Biopesticide

ABSTRACT

A nucleopolyhedrovirus (NPV), a composition comprising the NPV, and a method comprising the use of the NPV is provided. The NPV was isolated from  Cryptophlebia peltastica  and has insecticidal activity against several species of moths within the tortricid tribe, Grapholitini. The NPV or composition may be suitable for use in controlling insect populations, particularly populations of insects that infest plants.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a National Stage application of InternationalApplication No. PCT/162017/054543, filed 26 Jul. 2017. This applicationalso claims priority under 35 U.S.C. § 119 to South African provisionalpatent application number 2016/05197 filed on 26 Jul. 2016, which isincorporated by reference herein.

FIELD OF THE INVENTION

This invention relates to a biopesticide, more particularly anucleopolyhedrovirus (NPV), for use in controlling insect populations.

BACKGROUND TO THE INVENTION

Chemical pesticides are the most commonly used agents for controllinginsect pests in agriculture and forestry. Chemical insecticides may haveadverse impacts not only on their target organisms but also onbeneficial insects, such as bees and other pollinators. Accumulation ofchemical pesticides through the food chain of an ecosystem has also beenshown to have an adverse effect on birds and other insect predators.Furthermore, residues of chemical pesticides on fruit, vegetables andnuts may cause health problems among the human population.

Concerns over the use of chemical pesticides have led to an increasedinterest in biological pesticides. Insect baculoviruses are one group ofinsect biopesticides that have been promoted as an alternative controlagent to chemical pesticides. These viruses, which can be isolated froma variety of insect species, exhibit relatively narrow host ranges andhave almost no adverse environmental impact due to their hostspecificity. The absence of chemical residues means that long-termenvironmental hazards and health concerns are reduced withbiopesticides. However, there are several disadvantages tobiopesticides, including cost of production, efficacy and stability.

Nucleopolyhedrovirus (NPV) or alphabaculovirus is a genus ofbaculoviruses that infect insects, mostly moths and butterflies. NPVshave been known to be useful as biopesticides for insect-infested crops.NPVs have a high species specificity and while this enhances theirutility as biopesticides, it may limit their effectiveness in cases ofmultiple pest infestation. Furthermore, there are isolated incidents ofspecies of moth pests developing resistance to commercially-availablestrains of baculoviruses.

Cryptophlebia peltastica (also known as litchi moth) is a species ofmoth that is an important pest of litchi trees (Litchi chinensis) inSouthern Africa, Mauritius and elsewhere. C. peltastica larvae are knownto damage up to 20% of fruits in commercial orchards. This moth is alsoa pest of macadamia and a variety of other commercially significanttrees and plants. There are currently no known biopesticides thatspecifically target C. peltastica and to date, no NPVs have beenisolated from C. peltastica or from any other species within the samegenus or the lepidopteran tribe Grapholitini.

There is therefore a need for a biopesticide which is active againstCryptophlebia peltastica and/or other insect pests.

SUMMARY OF THE INVENTION

In accordance with a first aspect of the invention, there is provided anucleopolyhedrovirus (NPV) for use in controlling insect populations,the NPV having a genome sequence with at least 85% sequence identity toSEQ ID NO: 2.

The NPV may have a polyhedrin gene with more than 93% sequence identityto SEQ ID NO: 1.

The NPV may be an isolate or genotype of the species whose genomesequence is represented by SEQ ID NO: 2.

The NPV may have a virulence host range comprising the Grapholitinitribe of the lepidopteran family, Tortricidae, and the host range mayinclude Cryptophlebia peltastica, Thaumatotibia leucotreta, Cydiapomonella, Grapholita molesta, and Thaumatotibia batracopa.

The insects may be selected from the tribe Grapholitini, particularlythe larvae of Cryptophlebia peltastica, Thaumatotibia (Cryptophlebia)leucotreta and Cydia pomonella.

The NPV may be used in combination with one or more additionalbiopesticides, and the one or more additional biopesticide may be aCryptophlebia leucotreta granulovirus, a Cydia pomonella granulovirus, aCryptophlebia batracopa granulovirus or a Grapholita molestagranulovirus.

The NPV genome sequence may contain only one recognition site for therestriction enzyme Sma1.

In accordance with a second aspect of the invention, there is provided abiopesticidal composition comprising an NPV as described above and oneor more agronomically acceptable adjuvants and/or diluents.

The composition may comprise an additional biopesticide, which may be aCryptophlebia leucotreta granulovirus, a Cydia pomonella granulovirus, aCryptophlebia batracopa granulovirus or a Grapholita molestagranulovirus. The composition may further comprise a feeding stimulant,a mineral oil and a UV protectant.

In accordance with a third aspect of the invention, there is provided amethod of controlling insect populations, the method comprising applyingto the insects and/or their locus an insecticidally effective amount ofan NPV as described above or a biopesticidal composition as describedabove.

The method may comprise simultaneously or sequentially applying, inaddition to the NPV or biopesticidal composition, at least oneadditional biopesticide to the insects and/or their locus.

In accordance with a fourth aspect of the invention, there is provided amethod of treating or preventing insect infestations on plants or fruit,the method comprising applying an insecticidally effective amount of anNPV as described above or a biopesticidal composition as described aboveto the plants or fruit.

In accordance with a fifth aspect of the invention, there is provided anNPV isolated from Cryptophlebia peltastica, which has a genome sequencewith at least 85% sequence identity to SEQ ID NO: 2.

The NPV may have a polyhedrin gene with more than 93% sequence identityto SEQ ID NO: 1.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 illustrates transmission electron micrographs of NPV occlusionbodies (OBs) isolated from symptomatic C. peltastica larvae.

FIG. 2 illustrates a transmission electron micrograph of purified C.peltastica NPV OBs.

FIG. 3 illustrates transmission electron micrographs of OBs aftersectioning of resin-embedded samples.

FIG. 4 illustrates a 0.7% agarose gel electrophoresis used to observegenomic DNA of C. peltastica NPV. Lane 1: GeneRuler 1 Kbp DNA Ladder(Thermo Scientific®), Lane 3: NPV genomic DNA.

FIG. 5 illustrates a 1% agarose gel electrophoresis showing PCRamplification of the polyhedrin gene of C. peltastica NPV. Lane 1:GeneRuler 1 Kbp DNA Ladder (Thermo Scientific®), Lane 3: negativecontrol and Lane 5: ±700 bp positive control PCR product of concentratedCrleGV DNA, Lane 6: ±700 bp PCR product of 1:10 dilution of CrleGV DNA,Lane 8: concentrate DNA of C. peltastica NPV and Lane 9: 1:10 dilutionof C. peltastica NPV DNA.

FIG. 6 is a Sma1 restriction profile comparing an in silico digest of C.peltastica NPV and a REN digest of C. peltastica NPV.

FIG. 7 is a graph illustrating the infectivity of C. peltastica NPVagainst three European codling moth cultures with varying degrees ofresistance to Cydia pomonella granulovirus (CpGV): CpS (susceptible),CpRR1 (resistant) and Cp5M (more resistant).

DETAILED DESCRIPTION OF THE INVENTION

The invention provides a nucleopolyhedrovirus (NPV), also known as analphabaculovirus, a biopesticidal composition comprising the NPV, and amethod of controlling insect populations and infestations.

Throughout the specification, the term ‘plant’ is used in a generalsense and is understood to refer to trees, shrubs and bushes, as well asthe fruit, seeds and nuts of such trees, shrubs and bushes.

Moreover, the word ‘comprise’ or variations such as ‘comprises’ or‘comprising’ is understood to imply the inclusion of a stated integer orgroup of integers but not the exclusion of any other integer or group ofintegers.

Furthermore, ‘insecticidally effective amount’ is understood to refer tothose quantities of virus which will result in a significant mortalityrate in a treated group of insect pests as compared to an untreatedgroup.

The definition of a baculovirus species as herein described isunderstood to refer to all isolates and genotypes that have a K-2-Pdistance between single and/or concatenated polh, lef-8 and lef-9nucleotide sequences which is smaller than 0.015. K-2-P is the Kimura2-parameter distance (K), which is determined by the Kimura twoparameter model according to the following formula:

$K = {{- \frac{1}{2}}{\ln\left( {\left( {1 - {2p} - q} \right)\sqrt{1 - {2q}}} \right)}}$where p is the proportion of nucleotide sites that show transitionaldifferences and q is the proportion of nucleotide sites that showtransversional differences. Transitions refer to substitutions betweennucleotide bases of the same type (purine for purine or pyrimidine forpyrimidine), whereas transversions refer to substitutions of a purinefor a pyrimidine or a pyrimidine for a purine. Furthermore, two virusesare considered to be different virus species if the K-2-P distancebetween single and/or concatenated sequences is larger than 0.050. Fordistances between 0.015 and 0.050, complementary information is neededto determine whether two viruses are the same or different species. Thisdefinition is explained in further detail in Jehle et al Virology 346(2006) 180-193.

The NPV was isolated from larvae of the Cryptophlebia peltastica moth inwhich it proliferates and to which it is pathogenic. The virus producespolyhedrin proteins that form occlusion bodies (OBs or polyhedra), whichare globular structures that protect the virus particles from theoutside environment until they are ingested by an insect host. The NPVis characterized by its genome sequence which has at least 85% sequenceidentity to SEQ ID NO: 2. The NPV has a characteristic polyhedrin genethat has a nucleotide sequence with at least 75% sequence identity toSEQ ID NO: 1, with the proviso that SEQ ID NO: 1 does not encode theEpinotia granitalis baculovirus polyhedrin gene.

In some embodiments the NPV can be characterized by its genome sequencewhich can have at at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%,99% or 100% sequence identity with SEQ ID NO: 2.

In further embodiments, the virus can have a polyhedrin gene with asequence that has at least 80% sequence identity to SEQ ID NO: 1, and infurther embodiments the virus can have a polyhedrin gene with at least85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequenceidentity with SEQ ID NO: 1. In some embodiments, the virus has apolyhedrin gene with a nucleotide sequence that has more than 93%sequence identity to SEQ ID NO: 1.

The NPV can be characterised in that it is an isolate or genotype of theNPV species whose genome sequence is represented by SEQ ID NO: 2.

The NPV may have a virulence host range that comprises the Grapholitinitribe of the lepidopteran family, Tortricidae. In particular, the NPVcan have a virulence host range that includes members of theGrapholitini tribe selected from Cryptophlebia peltastica, Thaumatotibialeucotreta, Cydia pomonella, Grapholita molesta (oriental fruit moth),and Thaumatotibia batracopa (macadamia nut borer).

The nucleotide sequence of the NPV genome can have a characteristicsingle recognition site for the restriction enzyme, Sma1. This allowsthe NPV to be identified from other viruses that have more than one Sma1recognition site.

The NPV can be applied in combination with one or more additionalbiopesticides, simultaneously, sequentially, or in a combinedcomposition, in order to increase the spectrum of activity and to reducethe possibility of insect pests developing resistance to the virus. Inparticular, the NPV can be co-administered with another NPV or abetabaculovirus, also referred to as a granulovirus (GV), such as aCryptophlebia leucotreta granulovirus, a Cydia pomonella granulovirus, aCryptophlebia batracopa granulovirus or a Grapholita molestagranulovirus.

The NPV can be combined with one or more agronomically acceptablecomponents, including adjuvants, diluents, surfactants, dispersingagents, emulsifiers, spreading agents and/or wetting agents to form abiopesticidal composition. The NPV can also be formulated to improve thestability or maintain the activity of the virus.

In particular, a composition comprising the NPV may additionallycontain:

-   -   (i) one or more of the GVs referred to above;    -   (ii) one or more feeding stimulant selected from molasses,        sugar, syrup, alcohol sugars (such as mannitol and xylitol) and        Stevia leaf extract; and    -   (iii) one or more UV protectant.

In a preferred embodiment, the composition can be formulated to comprisemolasses, mineral oil, and one or more of the UV protectant Break-Thru®OE446, Uvinil and clove oil. In this and other embodiments, theformulation can further comprise glycerine and distilled water.

The minimum effective concentration of the virus is 1 OB, which can besufficient to kill 50% of first instar larvae in a treated sample.

The biopesticidal composition can be formulated to have a concentrationof the NPV of from about 1×10³ to about 1×10¹⁵ viral occlusionbodies/ml. In some embodiments, the biopesticidal composition can have aconcentration of the NPV of about 1×10¹⁰ viral OBs/ml.

A solution of the NPV can be produced on a commercial scale according tothe following method. Jars containing cooked larval diet are placed intoa biohazard chamber and sheets containing C. peltastica eggs eachcontaining approximately 400 eggs are sterilized with formalin beforebeing placed onto the diet in the rearing jars. The jars are thensealed. Approximately 80% of the rearing bottles containing 4^(th) and5^(th) instar larvae are removed. The remaining 20% of the rearingbottles have their lids replaced with a cotton wool plug under thebiohazard chamber and are left for a further ±8 d for the larvae to moveout of the diet and begin pupation in the cotton wool substrate.

The pupated larvae are then removed from the jars and placed intocontainers containing approximately 10 mm of larval diet. Once thelarvae cover the surface of the diet they are inoculated by spraying thesurface of the diet with viral inoculum. The jars are then closed with abreathable lid and monitored. The diet and infected larvae are thenharvested from the container after 7-8 days. The diet and harvestedlarvae are then homogenized in 4 litres of water per kilogram of dietand infected larvae (at pH 5.0), until the mixture appears uniformlysmooth (approximately 20 min at 2400 rpm). The resulting liquid issieved through a 100 micron nylon mesh into a clean container andre-homogenized after dilution with fresh water. The re-homogenizedmixture is re-sieved and stored in cooling vats at 4° C. Storage bottlescontaining glycerol and distilled water are prepared before being filledwith the virus suspension. The final product is stored at 4° C.

The biopesticidal composition can include one or more additionalbiopesticides, such as another NPV or GV. In some embodiments, theadditional biopesticide is a Cryptophlebia leucotreta granulovirus, aCydia pomonella granulovirus, a Cryptophlebia batracopa granulovirus ora Grapholita molesta granulovirus.

The NPV or composition is suitable for use as a biopesticide forcontrolling insect populations, particularly populations of moth andbutterfly larvae of the tribe Grapholitini. Although isolated from C.peltastica, the virus also has activity against a range of other insectpests and can be used as a biopesticide for controlling Thaumatotibialeucotreta (false codling moth) and Cydia pomonella (codling moth)species. Thaumatotibia leucotreta is sometimes referred to asCryptophlebia leucotreta and the two names can be used interchangeably.Furthermore, the virus can have activity against at least some strainsof codling moth larvae that are resistant to Cydia pomonellagranulovirus.

The NPV or composition can be applied to trees or plants as abiopesticide for treating or preventing insect infestations. Suitableexamples include citrus (all varieties), grape (all varieties), peaches,plums, nectarines, apples, pears, walnuts, quinces, hazelnuts, pecans,macadamia, avocado, litchis, lychees, peppers, ornamentals, cotton,karalla, olives, grape vines, pepper bushes, pomegranates andpersimmons.

The NPV or composition can be applied to the insects themselves and/orto the locus of the insects. For example, the NPV can be applied to thefruit, leaves, flowers and/or bark of the trees or plants which may beoccupied or anticipated to be occupied by insect pests. In someembodiments, the NPV can be applied by spraying, although any suitableapplication method can be used.

The insecticidal activity or effectiveness of the NPV arises when anocclusion body (OB) of the virus is ingested by an insect host duringfeeding. Once inside the insect gut, the polyhedrin sac of the OB issolubilised by action of the alkaline digestive juices (pH 9.5 to 11.5)and by enzymatic degradation, which releases viral particles. Primaryinfection occurs in cells in the gut, followed by other cell typesincluding haemocytes, tracheal cells, and fat cells. In advanced stagesof viral infection, the cells contain multiple viral particles which arepresent in the nucleus. Eventually the nucleus and the cell rupture,liberating a plurality of viral OBs. The external appearance of infectedlarvae changes during viral infection. The larvae become sluggish andtheir growth is stunted. As infection progresses, the integument of thelarvae may change colour. Eventually the integument ruptures and agreyish-white fluid is released containing multiple viral OBs. Whenhealthy larvae feed on plant parts that have been contaminated with thisfluid, they ingest the viral OBs and become infected themselves, leadingto further propagation of the virus.

The invention will now be described in more detail by way of thefollowing non-limiting examples.

Examples

Methods and Materials

A laboratory culture of C. peltastica was established at RhodesUniversity, Waainek research facility, South Africa. During rearing,larvae showing symptoms of ‘wilting’ disease were collected inmicrotubes and placed at −25° C. for future use. These symptoms wereassociated with colour change: milky pink appearance (early stage) andblack/brown appearance (late stage).

In an attempt to isolate a baculovirus from the symptomatic larvae, aprotocol for a granulovirus (GV) crude extract was adapted from Parnellet al. (2002). However, no virus was observed using this method andtherefore an NPV protocol adapted from Grzywacz et al. (2007) was usedinstead. Insect cadavers were homogenised in 1 ml of 0.1% SDS in amicrotube to disrupt the cuticle. The microtube was then vortexed forapproximately 2 minutes. The homogenate was then centrifuged at 100×gfor 10 to 20 seconds to remove insect debris. The supernatant wascollected and placed into a separate microtube. The pellet wasre-suspended in 1 ml 0.1% SDS and again centrifuged at 100×g for 10 to20 seconds. The supernatant was pooled and the pellet discarded. Thepooled supernatant was centrifuged at 2 500×g for 5 minutes to pelletthe virus. The supernatant was discarded and the pellet was re-suspendedin 1 ml ddH₂O and centrifuged at 2 500×g for 5 minutes. The supernatantwas again discarded and the pellet re-suspended in approximately 100 to200 μl ddH₂O.

Purification of Virus Occlusion Bodies by Glycerol GradientCentrifugation

To distinguish the morphology of the baculovirus, occlusion bodies (OBs)were purified using a glycerol gradient. A 50 to 60% glycerol gradientwas prepared in a microtube, following a method adapted from Grzywacz etal (2007), by placing 500 μl of 60% glycerol into a 2 ml tube overlaidwith 500 μl of 50% glycerol. The pellet from a crude extract wasre-suspended in 500 μl of 20% glycerol. 100 μl of this sample was thenplaced on top of the gradient and centrifuged at maximum speed for 15minutes. Both the band and pellet were collected and re-suspended in 1ml ddH₂O and centrifuged at maximum (12 100×g) speed for 15 minutes. Thesupernatant was discarded and the pellet was re-suspended andcentrifuged again as described above. The final pellet was re-suspendedin ddH₂O.

Transmission Electron Microscopy (TEM)

A drop of the prepared sample (±2 μl) was placed on a Forvar carboncoated grid for 30 seconds. Filter paper was used to drain off theexcess sample. A drop of uranyl acetate was then placed on the grid andleft for 20 seconds. Filter paper was used to remove the excess stainand the grid was left overnight. The grid was observed using a ZeissLibra 120 transmission electron microscope at 80 000 kV. The images wereanalysed using Mega View (G2) Olympus analyses software. The size, widthand diameter of the OBs was determined by measuring 100 OBs from theimages taken.

CTAB DNA Extraction

A CTAB DNA extraction protocol was adapted from Aspinall et al. (2002).Using 200 μl of crude/purified occlusion bodies extracted, 90 μl of 1Msodium carbonate (Na₂CO₃) was added and incubated at 37° C. for 30minutes. 120 μl Tris-HCl (1M, pH 6.8), 90 μl of 10% SDS and 50 μlproteinase K (25 mg/ml) were added and samples incubated for a further30 minutes at 37° C. 10 μl RNAse A (10 mg/ml) was then added andincubated for another 30 minutes at 37° C. The solution was thencentrifuged at 12 100×g for 3 minutes. The supernatant was transferredto a new 1.5 ml tube and the pellet was discarded. 400 μl of warm CTABbuffer was then added to the supernatant and incubated at 70° C. for 60minutes. 400 μl of 4° C. chloroform was added and the sample centrifugedat 6 500×g for 10 minutes. The upper phase was transferred into a new1.5 ml tube and 400 μl of −25° C. isopropanol was added. The sample wasthen left overnight at −25° C. The sample was then centrifuged at 12100×g for 20 minutes and the supernatant discarded. 1 ml of cold 70%ethanol was added to the pellet and the sample centrifuged at 12 100×gfor 5 minutes. The supernatant was discarded and the pellet wasincubated at 50° C., until dry. The pellet was re-suspended in 20 μl 10mM Tris-HCl (pH 8.0).

Polymerase Chain Reaction (PCR)

Degenerate oligonucleotide primers, prPH-1 and prPH-2 designed by Langeet al. (2004) were used to genetically characterise the isolated NPV(Table 1). The primers were used to amplify the polyhedrin gene usingDNA extracted from the purified OBs.

TABLE 1 Degenerate oligonucleotide primers for the partialamplification of the polH/gran gene region (Lange et al. 2004).Oligonucleotide name Sequence (5′ to 3′) Polh/granTGTAAAACGACGGCCAGTNRCNGARGAYCCNTT (prPH-1) SEQ ID NO: 3 (Polh/granCAGGAAACAGCTATGACCDGGNGCRAAYTCYTT (prPH-2) SEQ ID NO: 4 *N = C, A, T orG; R = A or G; Y = C or T; D = A, G or T

PCR reactions were set up for a positive control, negative control andC. peltastica NPV. The positive control consisted of DNA extracted fromCrleGV (Cryptophlebia leucotreta granulovirus) to ensure correctamplification with the degenerate primers and the negative controlcontained no DNA to determine the presence of contamination. The DNAconcentration for C. peltastica NPV was determined using a Nanodropspectrophotometer (Thermo Scientific®) to be approximately 84 ng/μl.This was considered when mixing the reagents.

PCR parameters used for the amplification of the polyhedrin gene wereadapted from Lange et al. (2004) in order to obtain a PCR product of the507 to 510 nucleotides for analysis. The PCR amplification was performedin a thermocycler (BIO-RAD®). The PCR products were analysed by 1% AGEand band sizes estimated using GeneRuler 1 Kbp DNA Ladder (ThermoScienticfic®) to confirm the presence and size of the virus gene.

Sequencing

The PCR products obtained were sequenced by Inqaba BiotechnologyIndustries (Pty) Ltd (South Africa). FinchTV® version 1.4.0 (GeospizaInc. 2004-2006) was used to view the chromatograms in order to clean-upambiguous nucleotides. Following the clean-up, sequences were subjectedto NCBI BLAST to determine a closely matched baculovirus sequence.Sequences were analysed in MEGA® 6 (Tamura et al. 2013) to determinesingle nucleotide polymorphisms (SNPs). Once SNPs were determined, thesequences were translated to determine if the SNPs resulted in aminoacid changes.

Results

Symptomology

Collection of symptomatic larvae from the culture began once the culturereached high densities. Symptomatic larvae were found randomly duringrearing, in medium to low densities depending on how crowded the larvaewere and the time the infected larvae were found. Symptomatic larvaewere found on the surface of the diet, within the diet and hanging fromthe sides of the containers. Early symptoms of the virus were observedby a colour change in the insects, commonly from milky pink tobrown-black. Once larvae had reached the brown/black stage they wereprone to rupturing as the cuticle became soft and liquefied. Caution wastherefore taken when these larvae were collected to avoid rupturing thecuticle.

Crude Baculovirus Extraction and Transmission Electron Microscopy

Transmission electron microscopy of a crude extract confirmed thepresence of a baculovirus. The OBs were observed as amorphous blackstructures clumped together. The images of the OBs show thecharacteristic features of a NPV (FIG. 1). The OBs varied in sizebetween 421 nm to 1263.2 nm. The mean size of 100 NPV OBs was found tobe 731.09±15.13 nm.

Purification of Virus Occlusion Bodies by Glycerol GradientCentrifugation

Glycerol gradient centrifugation was used to purify OBs from the crudeextract. A band forming on top of the gradient and the pellet werecollected and placed in separate microtubes. TEM was then completed onboth samples. Very few to no OBs were observed in the band, withnumerous pure OBs found in the pelleted sample.

The pelleted sample contained the majority of the NPV OBs. Even thoughthe sample was found in the pellet where the insect debris is usuallyfound, the sample was pure. OBs were observed and could be morphologicalcharacterised. As shown in FIG. 2 the varying sizes, circular shapes andclumping together of the NPV can be observed.

DNA Extraction Using NPV Genomic DNA

Genomic DNA was successfully extracted from C. peltastica NPV crudeextract of OBs. The DNA was analysed on 0.7% agarose gel electrophoresisand found to be of high molecular weight (>10 000 bp) (FIG. 4).

PCR Amplification of the Polyhedrin Gene Using Degenerate Primers

Genomic DNA obtained was used to amplify the polyhedrin gene usingdegenerate primers. The amplified product was analysed on 1% agarose gelelectrophoresis, illustrated in FIG. 5. There was no evidence ofcontamination as no band was present in the negative control (Lane 3).The positive control of CrleGV was successful as a ±700 base pairproduct was amplified (Lane 5 & 6). The polyhedrin gene of C. peltasticawas successfully amplified, as evidenced by the obtained PCR product ofapproximately 700 base pairs (Lane 8 & 9). All products were of goodquality, producing pure, tight bands.

Sequencing Analysis

Sequences obtained were subjected to a BLAST, using a blastn analysiswhere a closest match of 93% was observed with a partial sequence ofEpinotia granitalis baculovirus polyhedrin gene region (501nucleotides). After sequence clean-up the sequence for C. peltastica NPVpolH was found to be 507 nucleotides in length (SEQ ID NO:1). Followingtranslation and alignment between the two sequences, several SNPs wereobserved. Five of these SNPs resulted in amino acid changes: Isoleucineto Valine, Phenylalanine to Tyrosine, Serine to Alanine, Valine toIsoleucine and Glutamic acid to Aspartic acid. The remaining SNPs wereminor and did not result in any changes.

E. granitalis, the cypress bark moth, is native to Japan and is a pestof Japanese cedar and Japanese cypress (Takatsuka 2007). There islimited research on E. granitalis baculovirus and only a partialpolh/gran sequence is available for comparison. The reasons as to whythe polyhedrin gene sequences of these viruses are so closely matchedare unknown because they infect different insect species that are notfound in the same geographic region and they do not share the samehosts.

This is the first identification of a NPV infecting C. peltastica andfurther characterisation through whole genome sequencing and RENanalysis of genomic DNA was conducted to investigate the novel status ofthis virus.

Genomic Analysis of Cryptophlebia Peltastica Nucleopolyhedrovirus

Further genetic characterisation of the isolated NPV was carried outusing restriction endonuclease (REN) analysis and sequencing of the fullgenome. REN analysis is a technique used extensively in molecularbiology for genetic engineering, genome mapping and DNA sequenceanalysis (Roberts 1979; Bikandi et al. 2004). REN analysis is also animportant technique that can be used to compare the genotypes ofdifferent virus isolates for biological control purposes.

Restriction endonuclease analysis is used to create a genetic‘fingerprint’ for isolated viruses. In order to produce this‘fingerprint’ viral DNA is digested with the use of restriction enzymes.These enzymes cut the DNA strands at certain nucleotide sequences,resulting in several pieces of DNA strands. The fragments are thenanalysed by agarose gel electrophoresis, which separates them accordingto size creating a DNA profile. It is from these ‘fingerprints’ thatviral isolates can be characterised in terms of genotype.

Restriction Endonuclease Analysis

Genomic DNA was extracted and used to create REN profiles, using thefollowing fast digest restriction enzymes BamH1, EcoR1, Hind111, Kpn1,Pst1, Sal1, Sma1, Xba1 and Xho1 (Thermo Scientific®, USA). A 30 μlreaction was set up, comprising of 200 ng/μl of template DNA, 3 μl fastdigest restriction enzyme, 3 μl fast digest buffer and the remainingvolume made up with ddH₂O. The reaction was incubated at 37° C. for 15minutes. REN profiles were visualised using 0.6% agarose gelelectrophoresis, which were electrophoresed at 30 volts for 16 hours in1× TAE buffer and stained with ethidium bromide. Images of the RENprofiles were captured using UVIpro chemi (UVItec) UV trans-illuminator.To determine the band sizes two DNA ladders were used, a GeneRuler highrange ladder (Thermo Scientific®, USA) and GeneRuler 1 Kb DNA ladder(Thermo Scientific®, USA). UviBand software (UVItec) was used toestimate the band and genome sizes.

Restriction Endonuclease Analysis Profiles

None of the REN profiles of BamH1, EcoR1, Hind111, Kpn1, Pst1, Sal1,Xba1 and Xho1 produced a characteristic profile that could be used toeasily distinguish CrpeNPV from similar baculoviruses. However, the RENprofile produced by Sma1 digestion produced a characteristic profilecontaining a single band in the agarose gel electrophoresis gel (FIG.6). This indicates that there is only one recognition site in theCrpeNPV genome for Sma1, which can be used as a simple means of analysisand identification of virus isolates.

As no other profiles of known CrpeNPV analogues were available forcomparison, REN analysis could not be used to accurately characteriseCrpeNPV. Therefore the whole genome was sequenced for a more accuratemethod of genetic characterisation.

Genome Sequencing

The full genome (SEQ ID NO:2) of CrpeNPV, using approximately 100 ng ofDNA extracted from purified OBs, was sequenced by Inqaba BiotechnicalIndustries (Pty) Ltd (South Africa) using a MiSeq Desktop Sequencer(Illumina). The Illumina sequencing of the full genome of CrpeNPVproduced a total of 3 871 946 paired reads. Geneious (New Zealand)version R7 (Kearse et al. 2012) was used to complete a de novo assembly.In order to produce a de novo assembly, the ends of the paired readswere trimmed using the soft trimming function in Geneious. Once thepaired reads were trimmed a de novo assembly was run using 20% of thedata and numerous contigs were produced. The longest contig was selectedand a consensus sequence was generated to create a full genome sequencefor CrpeNPV.

Analysis of the CrpeNPV Genome

A total of 3 871 946 paired reads were produced from the Illuminasequencing. Of these reads 760 297 were used to complete a de novoassembly, which produced 104 532 contigs. The largest contig, contig 1,was used to create a consensus sequence of 116 646 bp. The consensussequence had a coverage of 731.6±390.8 (Mean±StDev) and a GC content of37.1%. The full genome of CrpeNPV (SEQ ID NO: 2) was annotated using adatabase of 18 NPV full genome reference sequences. A total of 105 geneswith complete coding regions (stop to start condons) were identified inthe genome. The 105 genes were labelled according to ORF numbers, ORF1starting at the polyhedrin gene.

The majority of the 31 core genes identified in all baculovirus genomewere recognised in the CrpeNPV genome with the exception of gp64, p6.9,odvp-6e, p95, vp80/97, gp67, p87, lef-6, ptp-2, ptp-2, arif, vef, pcna,ie-2, lef-7, pe38 and lef-3. The gp64 gene is a distinguishing featurefor NPVs, as NPVs are divided into two groups, group 1 and group 2.Group 1 NPVs express the gp64 gene and fusion protein, whereas group 2NPVs lack the gp64 gene but contain the fusion protein. Therefore theCrpeNPV is a group 2 NPV since it lacks the gp64 gene.

The results of the BLAST search revealed that there are no knownpolyhedrin genes having a nucleotide sequence greater than 93% sequenceidentity to SEQ ID NO: 1 (the CrpeNPV polyhedrin gene sequence).

Biological Activity

The bioassays were used to determine the virulence of CrpeNPV against C.peltastica, T. leucotreta and C. pomonella using a surface dose bioassaymethod. The first objective of the bioassay study was to determine theconcentration of the viral product required to cause 50% (LC₅₀) and 90%(LC₉₀) mortality by using a range of doses against all three species.The second objective was to determine the speed to kill 50% (LT₅₀) and90% (LT₉₀) of the population using the LC₉₀ value obtained from thedose-response assays.

Surface Dose-Response Bioassays with C. peltastica Neonate Larvae

Surface dose bioassays were conducted in glass vials. Each glass vialwas filled with 2 g of false codling moth (FCM) artificial diet and 2 mlof distilled water, the diet autoclaved at 121° C. for 13 minutes. Sixfive-fold dilutions of purified OBs of CrpeNPV in sterilised distilledwater were used for doses and sterile distilled water as a control.Forty eight larvae were treated per dose (48 glass vials per dose) andassays were replicated three times. A volume of 100 μl of each viraldilution and control was pipetted onto the centre of the diet surface.The fluid was spread evenly over the diet surface by rotating the vialat an angle. Inoculated glass vials were left for ±30 mins, until thediet had dried. One neonate larva was placed into each vial. All larvaewere from the same batch of eggs, hatching at the same time. Vials werethen closed with a lid and left upside down for approximately twelvehours to prevent larvae from escaping. Vials were kept in a CE room at27° C. and a relative humidity of 60-80%. Vials were then turnedupright. Approximately 24 hours later, lids were replaced with cottonwool.

After 7 days, vials were opened and diet was inspected. Larvae wererecorded as dead or alive. The dose-response curve was calculated usingPROBAN (Van Ark 1995) software used for probit analysis (Finney 1971).Mortality of control larvae were taken into consideration by PROBAN andcorrected using Abbott's formula (Abbott 1925). From the analysis, LC₅₀and LC₉₀ were calculated for each replicate. An average LC₅₀ and LC₉₀were obtained from the means from the three assays.

Surface Dose-Response Bioassays with FCM Neonate Larvae

Surface dose bioassays were conducted in 24 well plates. Diet wasprepared by adding 250 g of FCM artificial diet to a pyrex dish, 300 mlof dH₂O was added to the diet. The diet was sterilised in an oven at180° C. for 30 mins. Diet plugs were then cut out using a glass vial.Diet plugs were then pressed into the wells of the plate using a sterileglass rod. Six five-fold dilutions of purified OBs of CrpeNPV insterilised distilled water were used for doses and sterile distilledwater as a control. Forty eight larvae were treated per dose (two 24well plates per dose) and assays were replicated three times. A volumeof 50 μl of each viral dilution and control was pipetted onto the centreof the diet surface. The fluid was spread evenly over the diet surfaceby rotating the plates.

Inoculated plates were left for ±30 mins, until diet had dried. Oneneonate larva was placed into each well. All larvae were from the samebatch of eggs, hatching at the same time. Assay plates were sealed withparafilm and kept in a CE room at 27° C. and a relative humidity of60-80%.

After 7 days, plates were opened and diet was inspected. Larvae wererecorded as dead or alive. The dose-response curve was calculated usingPROBAN (Van Ark 1995) software used for probit analysis (Finney 1971).Mortality of control larvae were taken into consideration by PROBAN andcorrected using Abbott's formula (Abbott 1925). From the analysis, LC₅₀and LC₉₀ were calculated for each replicate. An average LC₅₀ and LC₉₀were obtained from the means from the three assays.

Surface Dose-Response Bioassays with Codling Moth Neonate Larvae

Surface dose bioassays were conducted in 24 well plates. Diet wasprepared by autoclaving 13 g of agar in 400 ml of distilled water.235.27 g of pre-mixed bollworm diet was sterilised in an oven at 180° C.for 10 mins. A 1527 μl of propionic acid and 154 μl of phosphoric acidwas mixed into the sterilised diet. Agar was then added to the diet, 200to 400 ml of boiling water was added and mixed to form a uniform paste.Diet was then piped into the 24 well plates. Five five-fold dilutions ofpurified OBs of CrpeNPV in sterilised distilled water was used for dosesand sterile distilled water as a control. Forty eight larvae weretreated per dose (two 24 well plates per dose) and assays werereplicated three times. A volume of 50 μl of each viral dilution andcontrol was pipetted onto the centre of the diet surface. The fluid wasspread evenly over the diet surface by rotating the plates. Inoculatedplates were left for ±30 mins, until diet had dried. One neonate larvawas placed into each well. All larvae were from the same batch of eggs,hatching at the same time. Wells were sealed with parafilm and kept in aCE room at 27° C. and a relative humidity of 60-80%.

After 7 days, plates were opened and diet was inspected. Larvae wererecorded as dead or alive. The dose-response curve was calculated usingPROBAN (Van Ark 1995) software used for probit analysis (Finney 1971).Mortality of control larvae were taken into consideration by PROBAN andcorrected using Abbott's formula (Abbott 1925). From the analysis, LC₅₀and LC₉₀ were calculated for each replicate. An average LC₅₀ and LC₉₀were obtained from the means from the three assays.

Time-Response Bioassays

Time response bioassays were conducted in glass vials. Fifty glass vialswere inoculated with 100 μl of sterile distilled water and 50 vials wereinoculated with the LC₉₀ (calculated from the dose-response bioassays)solution of purified CrpeNPV. The diet was left for ±30 mins, until ithad dried adequately. One neonate larva was placed into each vial. Vialswere sealed with a lid and the same process repeated as above and keptin a CE room at 27° C. and a relative humidity of 60-80%. Bioassays werereplicated three times. After 16 hours, the vials were inspected fordead larvae. Subsequently, vials were checked every 8 hours untilmortality had ceased. Diet was then dissected to determine whether theremaining vials contained dead or alive larvae. Time-response wasanalysed using a logit regression in STATISTICA version V12 (StatSoft2013). From the results obtained, the LT₅₀ and LT₉₀ were calculated.

Results of Surface Dose-Response Bioassays

The dose response curves for LC₅₀ and LC₉₀ were determined for eachspecies and isolate. The results are presented in Table 2 below.

TABLE 2 Comparison of the mean LC₅₀ and LC₉₀ values for C. peltastica,T. leucotreta and C. pomonella (South African) and C. pomonella(European, with isolates CpS and CpRR1 being susceptible and resistant,respectively, to CpGV). DOSE RESPONSE (OBs/ml) CrpeNPV CrleGV CpGV C.peltastica LC₅₀ 8.19 × 10³ — — (Litchi Moth) LC₉₀ 3.33 × 10⁵ — — T.leucotreta LC₅₀ 2.29 × 10³ 4.095 × 10³ — (False Codling LC₉₀ 9.97 × 10⁴1.185 × 10⁵ — Moth) C. pomonella LC₅₀ 1.43 × 10³ — 1.632 × 10³ (SouthAfrican LC₉₀ 1.26 × 10⁴ — 1.163 × 10⁵ Codling Moth) CpS LC₅₀ 2.70 × 10³LC₉₀ 2.86 × 10⁴ CpRR1 LC₅₀ 3.64 × 10³ LC₉₀ 3.24 × 10⁴

The virulence of CrpeNPV against T. leucotreta and C. pomonella wasslightly more fatal than that of their homologous baculoviruses, CrleGVand CpGV-SA. The LC₅₀ and LC₉₀ for CrleGV against its homologous host,T. leucotreta was found to be 4.095×10³ OBs/ml and 1.185×10⁵ OBs/mlrespectively (Moore et al. 2011). The LC₅₀ and LC₉₀ for CpGV-SA againstits homologous host, C. pomonella was found to be 1.632×10³ OBs/ml and1.163×10⁵ OBs/ml respectively (Motsoeneng 2016).

TABLE 3 Comparison of LC₅₀ values for European C. pomonella isolatesCpS, CpRR1 and Cp5M against isolates of CpGV. The CpS, CpRR1 and Cp5Misolates are susceptible, resistant, and more resistant, respectively,to CpGV. LC₅₀ (OBs/ml) for 14 days CpGV Isolates Cps CpRR1 Cp5M M 1.34 ×10² 4.46 × 10⁷ 1.88 × 10⁸ V015 9.94 × 10² 8.82 × 10² I07R 1.58 × 10³6.97 × 10³ 1.46 × 10⁷ I12 8.90 × 10² 1.40 × 10³ 7.76 × 10⁶ S 2.50 × 10³7.20 × 10² E2 9.50 × 10² 2.80 × 10² Madex Plus 5.26 × 10² 6.32 × 10⁶CrpeNPV 8.39 × 10² 1.39 × 10³

The results illustrated in Table 3 demonstrate comparable activity ofCrpeNPV against CpGV-sensitive and CpGV-resistant European codling mothisolates. M is a Mexican strain of CpGV; V015 is a resistance overcomingMexican isolate of CpGV; I07R and I12 are Iranian isolates of CpGV; S isan isolate of CpGV isolated from the commercial product, Virosoft; E2 isan English isolate of CpGV; Madex Plus is a laboratory selected isolatefrom CpGV-resistant codling moth.

Results of Time-Response Bioassays

Speed to kill values LT₅₀ and LT₉₀ were determined for each species andare illustrated in Table 4 below.

C. peltastica had an LT₅₀ of 73.44 hours and LT₉₀ of 89.21 hours.

T. leucotreta had a LT₅₀ of 80.69 hours and LT₉₀ of 96.37 hours.

C. pomonella had a LT₅₀ of 106.09 and LT₉₀ of 125.52 hours.

TABLE 4 Comparison of the mean LT₅₀ and LT₉₀ values for C. peltastica,T. leucotreta and C. pomonella. TIME RESPONSE (at LC₉₀ dose) CrpeNPVCrleGV CpGV C. peltastica LT₅₀ 73.44 hours — — (Litchi Moth) LT₉₀ 89.21hours — — T. leucotreta LT₅₀ 80.69 hours 118 hours — (False Codling LT₉₀96.37 hours 176 hours — Moth) C. pomonella LT₅₀ 106.09 hours  — 135hours (Codling Moth) LT₉₀ 125.52 hours  — —

Speed to kill values LT₅₀ and LT₉₀ were also determined for two Cydiapomonella cultures and against four viral isolates, as illustrated inTable 5 below.

TABLE 5 Comparison of LT50 and LT90 values for two Cydia pomonellacultures; CpS (susceptible) and Cp5M (resistant) against four viralisolates; CpGV-S, CpGV-E2, CpGV-M and CrpeNPV. CpS CpR5M LT₅₀ LT₉₀ LT₅₀LT₉₀ CpGV-S 139.28 183.55 — — CpGV-E2 107.68 132.07 126.89 156.48 CpGV-M115.58 148.74 200.00 — CrpeNPV 120.34 147.49 110.92 135.18

The speed of kill for CrpeNPV against the heterologous hosts was greaterthan that of their homologous granuloviruses (Table 4). As the LT₅₀ andLT₉₀ for CrleGV against T. leucotreta was observed to be 118 hours and176 hours (Moore et al. 2011) and a LT₅₀ of 135 hours for CpGV-SAagainst C. pomonella (Motsoeneng 2016).

From the above results it was established that CrpeNPV was more virulentagainst the neonate T. leucotreta and C. pomonella larvae, specificallywith a significantly faster speed of kill for both species. The mean LCand LT values were slightly less than for their own homologousbaculovirus, CrleGV and CpGV-SA.

Test for Infectivity

Two discriminating virus concentrations (5.8×10⁴ and 5.8×10⁵ OBs/ml)were used to test the infectivity of CrpeNPV against three Europeancodling moth (C. pomonella) cultures with varying susceptibilities toCydia pomonella granulosis virus (CpGV): CpS (susceptible), CpRR1(resistant) and Cp5M (more resistant). CrpeNPV caused 100% mortality forall three cultures at 5.8×10⁴ and 5.8×10⁵ OBs/ml (see FIG. 7). Assayswere also performed to determine the LC50 and LC90 values after sevenand fourteen days incubation time and the results are presented inTables 6 and 7 below.

TABLE 6 Comparison of the LC50 and LC90 values for the three Europeancodling moth cultures: CpS, CpRR1 and CpR5M after seven days Dose(OBs/ml) CpS LC₅₀ 2.70 × 10³ LC₉₀ 2.86 × 10⁴ CpRR1 LC₅₀ 3.64 × 10³ LC₉₀3.24 × 10⁴ Cp5M LC₅₀ 2.19 × 10³ LC₉₀ 2.32 × 10⁴

TABLE 7 Comparison of the LC50 and LC90 values for the three Europeancodling moth cultures: CpS, CpRR1 and CpR5M after fourteen days Dose(OBs/ml) CpS LC₅₀ 8.39 × 10² LC₉₀ 7.98 × 10³ CpRR1 LC₅₀ 1.39 × 10³ LC₉₀1.69 × 10⁴ Cp5M LC₅₀ 7.02 × 10² LC₉₀ 4.68 × 10³

These results indicate that CrpeNPV has activity against a wide hostrange including CpGV-resistant species of moth pests. This could beuseful when multiple lepidopterans infest the same host because not onlywill the primary pest be controlled but also the secondary pests. Thesecondary pests are usually only observed once the primary pest has beensuppressed.

The biological activity of CrpeNPV was found to be more virulent againstthe heterologous hosts including CpGV-resistant codling moth specieswith a faster speed of kill. These results indicate that CrpeNPV has thepotential to be commercially developed into a biopesticide forcontrolling insect populations of C. peltastica, T. leucotreta and C.pomonella in trees and plants, particularly in fruit (e.g. litchi,citrus, apple and pear) and nut (e.g. macadamia and walnut) trees.Additionally, CrpeNPV could also be used to control other lepidopteranpests in the Tortricidae family.

TABLE 8 Nucleotide sequence of C. peltastica NPV poly-hedrin gene (polH)(SEQ ID NO: 1)ACTTACTTTGTTTAAAGAAATCCGTAGCGTTAAGCCCGACACAATGAAACTAGTTGTTAATTGGAGCGGTAAAGAGTTTTTGAGGGAAACTTGGACTCGTTTCATGGAGGACAGCTTTCCCATTGTAAACGACCAAGAAATTATGGATGTTTTCCTAGTAGTTAACATGCGCCCGACTAGACCTAACCGTTGCTACAAGTTCTTGGCTCAACACGCTCTTCGTTGCGACCCAGACTATGTGCCTCACGAAGTAATTAGAATAGTTGAGCCGTCATGGGTGGGAAGCAACAACGAGTACCGTATTAGTCTGGCCAAGCGCGGCGGCGGATGTCCAGTGATGAATCTGCATGCTGAGTACACCAACTCGTTTGAAGAGTTCATTAACAGAGTCATTTGGGAAAACTTCTACAAGCCCATTGTGTACATTGGTACCGACTCTGCTGAGGAAGAGGAAATTCTAATGGAAGTTTCCCTGGTGTTCAAAGTAAAGGATTTCGCCC CGGGCCATACCTGTTTTTCGG

TABLE 9Nucleotide sequence of the C. peltastica NPV genome (SEQ ID NO: 2)ATGTATACGCGTTACAGTTATAACCCATCTTTGGGTCGTACGTATGTTTATGACAACAAGTATTACAAAAACCTCGGCTCAGTAATCAAAAACGCCAAACGTAAGCGCCACCTACTCGAGCATGAAATCGAGGAACGTAATTTGGACCCCCTAGATAATTATCTGGTCGCCGAGGATCCTTTCCTGGGACCCGGCAAAAACCAGAAACTTACTTTGTTTAAAGAAATCCGTAGCGTTAAGCCCGACACAATGAAACTAGTTGTTAATTGGAGCGGTAAAGAGTTTTTGAGGGAAACTTGGACTCGTTTCATGGAGGACAGCTTTCCCATTGTAAACGACCAAGAAATTATGGATGTTTTCCTAGTAGTTAACATGCGCCCGACTAGACCTAACCGTTGCTACAAGTTCTTGGCTCAACACGCTCTTCGTTGCGACCCAGACTATGTGCCTCACGAAGTAATTAGAATAGTTGAGCCGTCATGGGTGGGAAGCAACAACGAGTACCGTATTAGTCTGGCCAAGCGCGGCGGCGGATGTCCAGTGATGAATCTGCATGCTGAGTACACCAACTCGTTTGAAGAGTTCATTAACAGAGTCATTTGGGAAAACTTCTACAAGCCCATTGTGTACATTGGTACCGACTCTGCTGAGGAAGAGGAAATTCTAATGGAAGTTTCCCTGGTGTTCAAAGTAAAGGAGTTTGCTCCTGATGCTCCTTTGTACACCGGACCAGCGTATTAAACAATAGATTGCGCAGGTTTCATAATTTTCAATAGTCTTTGCTGTAGTTGAAGCAAAGGTGCCGAAGCGGGTTTCGCTTGTAAAGCCTTGTTGATAAAATCCATAGCATTTTGATATTTCTCGTGTAAAATTATGAATTTTACTTCCCTAAGAAATTGGGCTTCGTTTTCGTTATATAAATTTGTGGTGCTCGGAGTTTGGTTTTCTAAGATATTAATATTTTTTAGTCTTTTAACAATCGAATTAATAATTTCGTCTGCCGAATTTAAATTTTGCTCGTTTTCGTACGGAATATTGATTAAACGACTCGCTTCGGAGATTTCGTTAGCAAAACTATTATCGCTAATTTCTGTTAATATTACTAATTTTGAGGCTAGCGCATTCTTTCTATCCCGGTACTCTGTTTTATCATCATCGTCCCACTCATCGTTAGTCGTGTTTTCGTTTTCGGAAGCCGTACTAACAGGTTCTAAGGCTATACGTCTACTCATAATTTGAGCGACATGTTCGATACCTTTTGTTTCTTTTAGAGGTTCTTTTTCTATTTCAACCTTTTTCAATGTAGGTCTTTTCTTAATAGCCTCTAATAGGGCGCTTCTGGGGTCTACTGTAGTTTCAGGTTTAGGTGGGGTAGAACGTTCTGCGGCAGATTTTAATGCTGGCCTAGATCTTATTTGTTCCATTAGTTTACTAGGGTCTAAAGGCGATTCGGAAGGTTTTGGAGGAGACGGTGTCGATGTTTCTAAACCCGACATTGGCGGAGCAGGCGGTGCCTCGCTAGCTGTGGGCATAGGCGGTGGGGGCGGAGGACCAAAATCTGAAGCCATCGGCGGAGGCGGAGGAGGAACAAAATCTGAAACCATCGGCGGAGGCGGAGGCGGCGGTGGTGGTATGCTCGTTTCTAGCTCTTTCATAGGAGGCGGCGGAGGTATGTTTGTAATAGGTTCAGGAGGCGGCGGAGGCGGCGGTGGCATGAAAGGTTCAACTATTTTATCGTTTACCGTAGATATAAAATCGTTGGATGTAAGTAGAATGTCTTCCACACGTTTAACTAACTCACCATAGTTTTGATCGGGAATAACAGTTTTAATGTTTTGATCATCAGTTTCGTTCGAAACAATTGGTGCTACTATCGTTTCCCTGTTCAAACTTTTGTCGGCGCCTTTTGTGGCGATATCATCCAACGAAACTATATCATTGAGAATGCTATCGATCTTTTCGGTGTCCTTTTGAATCTTTTGGTTGTGATCGTGTAAATATTTTTTATACACGACAAAAAATTGTTTATAGTTATCTTCTACGTTTGTTTCATTTTGAATTTCGTCAATTATCGAAAGTAGCAATGATTTATTTTCATCGTTGTCGTCTACTTTTCTGGCAATGTTTATTAAAATTTTGACTTTATTATCAACCGATTTAACTATGGGCAACGTCGTTTCTGTTTCTACATGACTTACCCTTTCCTGCTCATAAATTATGGCAGTGTTATTGTAAATATCATTGGCAAGTTGAATCAATTCTAACGACTCTGCTAAAGTAAGTTTCAGCAGTTCGTTTTTGGGAGTAGCAAAAACTTTACGTTTCAGTTTATTTATTTGGGGACTTTTGATCATATGCATTAATTTGATAGGTTCAATTAAAGATTCATGTATAAAATAATCTCTGGCGGATTGTATGTCAGTTCGGTCTAGTCTTTTCTCAAACATGGACATTGTGGTTGACGATTTAGACGAGTTTAAACAAAATTTAACTCTAGAAAAAAATATTAAACTTATAAATGGAAAATTTGGCAAAGTGTCCGTGTGGCGCCACGAGCCCACTAAAAAACTATTTTTACTGAAGCAAATAACTCATGGTTATAATGAAATCGAACCTATGATTCATTATTTGATGAAAAAAAACAAGTACTTTTTAAATCTTTACTACTCTGTCAATTCGCCAACTACTAATATACTTATTATGGACTACATCGCCGATGGTGACCTGTTTGATCTTCTGCGCCGCGAGTCCCGCTTGCCCGAACCCGAAGTCCGCAGCATTATTTTACAGCTAGTCGACGCTCTACAAGCCCTACATAAAAACTTCATTATACATAACGATGTCAAGCTGGAAAATGTTTTGTACCATAGAAAGAGACAAATTTATTTGTGTGACTATGGACTCTGCCGCATAGTCGGAGTCAAGTCTTGCCAAGACGGCACTTTAGATTATTTCTCACCCGAAAAAATCATGGGAAGAAACTATGACTTTGGATTTGACTGGTGGTCAGTCGGTGTTCTAACCTACGAGCTAATCACAGGAACCCATCCATACAAATTTGATCCGGACGAAGACCTCACTACAAATGTTCTGCAGCGAAGGCAGCAAAGAAAACTTGAATTTAAACCTAGCGTGTCTATAGCTGCTCAGTCTTTTATTGAAAAATTACTGTTTTATAATATTAACTATAGATTAACTAGTGGTGATGAAATAAAATTAAACAAGTTTTTGCAATAAAAAAATTTATTTCATTTATAACTATAGTACCCTATACAAAATACTAGAGAATACAGGTTATTAATACTAATACTATGCTAAGTTTATACAATAAATGCTTAAATGTTAGTACACTTAAATTTTTTGTCGTGTTCCGTATGAATATGATTAACAATGATATCTGACATGCAATCTGTATTTAACAATTTGTGGGACACATTTGACAAAAATATGTTACGTTTTTTAAGTTCCTGAAACTTTTGTTTAACAATTTCATCGTTAGTTTTATATAAAACTTGCCGCTGGTTACAAATTTGCTGCGGCTTAGGCATAGGATCAGCACTGACAATGATGACATCATCATCGTCGTCATCATTGTCAGCCACATTAATAACTTTAACCGATTCGTCAATTACTTCAACGTCCTCATCGGGTTCTTTTTTAATTATAGGCTCGTTATCTTGAACTATGGCAGAAATTACAAAAGGTTCGTCATCAGCGGGCTCGGTTTTAATTACGCGCGAAGTTGACATTGTAGTTATTAAATCGGAAATGTCAGCTGTCTTATGTGACTTATGTGATTTAGATTTGCGCGATTTTGACTTTTTAGTTTTCTCTGGCAGCGGCTCGGGCGCCAATATGTGGTTACGGTCTAAATTCTGGTCTAAAATCAGGTTCTCCACAGCCGCTGCGGTAGAATCATCAAACTGAGCTAAAACGTCTTCAAAGTTAATAATAGGTATAGCGGGATTGTTAGAATCAAATATGTATGATTCCTCTGGTTCGCGAACCGAAGCCGGCTCGTTTTTCATAATATATATTTTGTTATTTTTCTCAAAGTATTTGACCCCATTGTGTATTATTTCTTGCGCCTCACTGACAAAATCTGTTAGATTTTTACCTCTAATCGCGTCAGACCTATCAATTGCCGGGGCCGGTTGGTCACCAAGAATTTGTTCCAATTCTTGGGCAGGTTGTTCACCGAGAATTAGTTCTAATTGTTTTTCTTGCTCCAGAATAGCCTGCCTTTCTCTTTCACACTTGTTTATTTTTTTTTGAAGCTCGATAATGGCGGCTTCATTGTCGGTGTTAGATGTACGAATCACACTACGGCTACGATCAGTATTAGCGACTTTTACCTTTAATCCTTTTTTAGGTTTTCTGTTACGGCTGCGGCTGCGTCTTTCATGGACAACGGCGGGGCTGTAAGGTTCATTTTGTCGCTCAGATCGAGCGGGGCTGAGGCTTTGCTGAGGTTGGTCGGGACTAGGGCTACGGCGGCTGAAGGTAGGACTGGGGCTGCGGTGGTTGGCAGGGCTGGGGCTGCGGCGGGTTGCGGCTGAATGCTGGCTGAAGGCGGGGCTAGGCGTGCGCTCGTTCGAACCAAAACGATGTCCGACACAAGGACTCGGAGTTCTGGTTCTGTCATTGCGGGCAGGGGTCGGGTCCCTGCTTAAAGCGGGACTCGGAGTTCTGGTACGGTCGCCATTGCGTGACCCGCTCTGGCTGCGAGAGCGAGATCTGCTTTTGCGCTCACTAGATTGGCTGCGGGAACGGCTTCTACACGTTTCGCTCGATTGGCTGCGGGAACGGTCGCTGTCGTCAGCGACGGAACGCTGGCCAGAAGTGGACCGGCGACTTTTACGAATAGAAACGGACCGGGATCGGTCTGATGTCTCGCTGCCGGAAGGACTTGAAGATCGGGTTCGGTTGCCATGGTTAATAATATCGCTTTGGTCGCTTTCGTGTGAACTGTTACTATAACTACGTTTACTGACACGACTATCGGTACGGTTTAACGCGGGCTTCTTTTTTCCTTTTTTTAGAATTACATTATCTTTAAGGCCTCGCGAAATGGCAATGGCTTCGGCCACTTCAAATGGGGTTTCTTCGCGTTTATAGTTACATTTAATACACGCGCCTCTCTGATAACTTTCAATGAAACAATCGCTGCACAATTTATGTTCGCAGTTTTTGTAAAAGCTTCTAATAAAAGATTTTTTACACGCCTCGCATATTATAGTTTGCAATTCTTTATTACCGTTACATTGACTGATTATTTCAGAACTGATTTTAATTTTAACTAAATTATTAATTTTATTGATAAGATCTTTCCGAAGATTTTCAATGGCAGTGAGGCCACATTTGTCCATAATCCTAATATGTTCTCGAATTTCATAGGTCGCGCCTATGTTACGCAAGTTTTTAGGAACGTGTTGAATCTGTTCGTAAAAAAACTCGCTAGAAGCAATAAAGTCGTGTTTAGACCCATTGGACATCTCCAACAATACGTTATGGACCGTGCCGAAAGCGCTGTTATTATCTCCTAAAGTGGGATAGTAATGATTGTAAAAGGCTCCATAACACAGTCTGAGATGAATCAAAACATCAAACTGAGTCAGACACGTCAGTAAAGTTTGTAAGTCTTTTTTTACTTGGAGGTTAGGCGATTCTATAATGTAATTATAGATGTTTTCATAGTTTCGTGTCAGTAAATTACGATTTTTAAAGGTAATACCCATAAATTTAAACGTATTAGTATTAGTGTAGTCTAAAAAGGCGACCTTTTTTTTCCCGGCTTGAAATTCAAGATAGAAATAAAGCCTGCCCTCAGGTTTATTATTAACCATTTGCAACTTTTTGTACGAGTTCTGAAAATAAAGGAAAAAACTTAGAGTTTGTCACTCTAAAAAAAAAAAACACAAAATTATTTACAAGTCCAAAAATGGGAAACATAAAAAAACACTGTTACTCACATCAAGTAGACGGCTCATCCTGTGCACTTCACTGGACTTTGCAGGGAGAAGAGGTGTTGCGTGTTCGAAGCTCGAAACGAAACTGAATCCAAGTTTCGCTACCAAGGCTTATATACCCTGGCTGGCGACCTTGTGGGCGTAATCAGTCACGCGGCTGCGGGCGTAAACAAAGTCAGTCACGTACGCCCTGACCTCCAGACGAACGGAACAAACAACCTAATCAGCAGCGCTTATCGGCGCATGACCGGAGATTGTTATCCGGTCACGTAGGCGCTCGTTTGTCACGTAGGCGCTTGTTTGTCACGTACGCCAGGAAGGTGATAAGCAGTTTTTTGCTCTACAAAAAAGGACCTAAAATCGTAGTATATTGGGAGCATATTGTACAGTGTAGACTATTATAGTAAAATAGTCTACGATATGAAATATTCCACTGTATATTAGCTGTAAGGACTTAAGTAACTTTTTTGCACTGCAAAAAAATGACCTTTCGATTCTGGACTAGTCGTGCTTGATTAGATTAGGTTTCGTATAAAAGGCCTGACAAATTGAACACGAATCATACTTTCGTACGGAGCCCTACATACCAACAGATAACCGGCCGTAGCATCATGAACAAAGTGAGTATTCTTGTGTATTATTTTTGTGCATATCCTGTCAGACATAGTTTGTTCGTTTTATTTATTTTGTTTTTTGTTTCAGATCGACAAAAAATCTGTTACCAAAATCGAATTATTTAAACCTACTATTTACAGCCTGAGAAAAAATAACAAAGATTTTGTCCATCACACCGAATTTACTAACCTCATGTTCAATAATTTAATTCGTCAGTATAAAATTTCTGAAAAAGAAACTGGCGACGCAAAAAAATTACGCCAAATAGATGCTTTTAATTTTTGTCGTTACATCCAAAATTACTACGAATTTAACACAGATCAAAGTTCTTTTTATTTGTATAATATCAGAACTGCCGTTATTAAATATGATTTGATTTTAAGGATGGAAAAGTTTAAAAAATTTGTTAAAAGGCGCTACTTCAAAGAACATGGCGTACGTATGCATCAGGATAAAGTTATGTGTCCTGACAAACATTACGTGGACATTTACAAACCTAAACTTACAAAAATTCACCTCACAAGTAAAGAAGAAAAATATTCGGACGACTCTGATCTGGACAGCTCGGAGGATGAAGGCTTCGGAGGACTACGCCAGCGACCTCGGTCTCCTATTAGGTTTAGGATTGTAAATAACACGAGAGTAATGTCGCGTTACTATCCTCAGTATTAATTATAACAGTATTTTATAGTATTACAGTATTATATAGTATTCAATAAACATTATTATGGTATTTTTTGTTTCAATTATCTCGCCATAGGTTCTAGAGGTTTGGTTAACAATTGTTTAAAAATAAAAATGCCAATGGCAAGCATAATTAATAAACCCCCTACCAACATGATTAAAGGCAAAAGTTTTTCTCCCAAACTTTTACTTTTATTCATGGATTTGTTAACCAGACCTTCTTCTCCGAGCAAATGATCTAGACCTAAATCTCCTATCAGATCTCCTAAATTGTAGGGTTCTATACATTGGATAGTTTGGCCGACAGACAAATCAGAAATGTCAACATACTGAGGTGACTCTGGGTCGGCATTAGGATCGCTAGCTCTACAAACTGTCTGTTCTGTTTCGTAATTAAACCCCCTGCAAAGTTCCAGCAGCTCTTGCTCATTCGAAATTAGCGGGTCGAATTCGCATAAAACTACTCTGTCCATTGCGTCCGCATTCAGCTGACAGGTTCTGTGTCTGAGCATACAAACAGTCATATCATCGCCTCCGTTAAAACCCACATAAAAATAGCTACCGCCAGTGTTATTCAATGCATTGATTATGTCCTGAATTAAGCTAGCGGTGCTAAATGCCAAATATACACCCGTACCGATGAGAGCTGTAACGCCTGCAAGTTTTAAATTTTCTAAGTATGTGCGCAAACGAGGCTGTTGAGTAAGCACAGTTTCCACACCGTTTGCGTTTCGTGTGTTTGTAGACGGATAGTTTTGTTTAACCGCATTTTTACGCAAATTAATGGAATGTAAATTAGCATCGGGAACATTGTCTATACGGCGTAAAGATCCTAAAGAATTTAACTGGCCGTTAGTTGCGTCGGGAAACATGGTTCGTAAGTTTGTACTGTCATTATTGCGCATAAATCGATTCATGTCAGCGACACTAACAAAATCATTGTTTCTGACCAAATAACCTGGCATGTACATGTCATTGTTTAGGTTTCGAAAACTTGAAGACGAAAAAACGTTTTGAAAACCAGCAGGGGAGGAGTTGACGACATTTAAATTGTCTACATTAAGAAATTGATTAGGGTTATTATAAACTTTATTAACTCTACGCAATGAGGTAAAGAAACTCATTTTTCCTTATTTACTTTTTAGGCTTAGTTCTACGCGTTTCAGGCGTAGGTTCAGGCTCGGGCTCCGGTTCTGGCTCAGGTTCCGGCTCTGGCTCAGGTTCGGGCTCCGGCTCTGGGTCGAGATCAGGATTAAGAGTTTCATCAATTTTATCTACGGTAGTTTGGATAGTTTGTAGAGTCTCTGACTGAGCCTGCAGTGCCTCATTAATTTCGGACACATCTGGCAGATTGGCTCTGATGTCTTGCACCGATGCCTGTAGCGCCGCGACTTTAGTGTCCACGGCTTTAATATCGGATCGAATCAAAACCAAAATATTTTGGGACATGATTATTTAATATACAAATGATTATGTATTATAACTTATTAACGGCCCGTCAAACCTCACCCTGTCAATTTCGTATTCACCCTCTGATAAAGTTATAACAATTTCGTGGTCGTTATAAAAAACATTAATATTTCTGGGCAAATCTCGATACGTATTTTTATTTTGATCAACAGTTAACGCATACCTTTTGAGCATGAAACTAGAATCGCCAACGTACGGCAATTGTTTTGTACCATACACGGACATACCTTGCAAAAATTTATGGAACGAAACAGATTTATCAAAGTTCATTTGACCGGACACTAATTTCTCGGGACGTATGCCCGTTATAGGAAAAATGACCAGGCCGTTTTTATAGTCATTGTGACGACAAGTAATTACTGAAATTAGTCGATTTTCTCTAAAAACGGGCGCGCCCAAGTAAATTTTGTTTGCCAGACTAAAATCAGTGAGTGCAAACGCATACATTTGACCATAAATTAACCTTTGTTTGTGGATATGAAAATTTGCATACACACGATCCACCGTAAAACTAGCCAGATCGCCGTTATTTAACAAAACATCTAACGCGGAATTGTTCGATACTTTAGGGAACACAATATTGCTCGCTACGCCGGGAAATTGGTGCAGCAGACCATATTCCTCGTCGTGTACACGGCTCATGGGCGCTATGATTTTAATAAACGATTCTTGCCCGTCAACTTTTGTTATTGTAACAGTCTTTTTTAGCTCGTTTTTTTCATAGTTAACATTATTAATTTCTAAATTTTGACCGTTTCTGATACAGAGCAATAGCAATAACAAAAATGTCATTGTCCTTTTTATAATCATATCTTATTAGTTCTTTAACCTTTGATAAAAATTACTATATAATTGTCTCGTTTCTCGAGATGAATCACAGAATCACACAGCATCTTAAGATATATCCATCATCATGCCGGTTACAAAATGCAAGCAAATACCCGCCTACCAGTTTATTCAAAAACTCAATTCATTGCTACGCAACATTAGTTCTGTGGAAAAACGGATCGATCATTGGAAGAGAATTAAACGGATTACAAAGGATAAACGAGAACTCATCGAAATAGATTGTATGCTGGCAAAAATGGATGCTGAACTTAGATCACTAAGAAACGATGTATTGGTTTTAAACAGTAAATAAAATAAAATATTTTTATAAATTGTATTTTATTCAATATCATCTTCACAAACTTCCACATCAATTATAGATCCATTTTCGTACCAATCTTCATCCACGTTTTCGCTAGTTTCCACCGCTGCAGGCGTTTTCTCCGGTATTTTGACTCGATGCCGATTATTATGAAATCCGTTTCTAACCAAATAGCATTTAAAAGGTTCACCACTGGGCCAGTAAACACGCCGGTTCACATTCAACAAGCGGTTAGCCGTATAGCGGCAATGTAGTATTAATTTTTTGCGCGTACAGGAATCCCAGTCAATATCGAGCCTACCGCCGCAAACATGGTTAAGAAACTCTTTGGTGAACCTTTTGGCATAGTTGGTACCGTTAATGTGAAATATGTACATTTTTAATCGGTTTGCTCGATATTGAAAAATATCATAGGTTTGATGGCCAACGAGTATGTTTTCTCTTGGTACTTCTTATTGTTAACACTTTCCAACGTAGTTTCGTTCTTACTCTGCTTGAATCCCTCAATTATAGCGCCCATATACAGATCCACTTCTTGAGAGGGCTGTGCTTGTTCTCCGCCTTCTTGTTGGAACGCGAATAAATCGTTGTAACGTTCCACGGTAAATGGCTCACACACAACGCTTTTGACCAAGTCTCCTGTGCTATAAACGGTCTCGTTGCTGTGCTGCTTTATATCATAAAATTTTCGCACAAACGAGATCTTACCCGGGTTATTTTCATCGGGCGCGTTTATTATGGAACAGTCTTGCAATTTTATTTGGTCCTCTTTGAAATATTGTGACATGATGTTGGAATAGATCATATTATGCCGATTGATATTGGACCAGCGCAGAATAAAGAACTCACCAAAAGTACTTTTGTAGCGCTTCATTTTCACCGTATCGAAAAAATAAAACGGTATAGCTCCTCCTTTGACATGATAACCCACGTTGTACACCACTCTAGGCGCTTTAGGGGTTTCTATGGTGATCTTGTCATCGATCTTTGGATACAAACGGCTTTCCCATCGAGAAAAGTTGTTGCACGTTTCCAAAAATCCTAGCGCCGTTTCCAATTGATTCTGATTAAACTCTGTCTGGCAATTAACCACCGTGATGTTCTTGCCCTTGGCCAAATTGTGTATCATTTTTTCGACCCATGTCATGGCACGCGTCTCTTCACGATTTTTAAAAATGCAAATCAACTTATCGTTAGCGTCATATTCCACTTCGGTTTTGTAAATGTCCAAACTATCGCAGCTTTTTTTGGACACCGACATACCGTCCGAGGAATCGCTTGATCTACGCTTGGTAGCCATTATTAAAATAATGTTGCACTTAATTTAAGGTTACTATTTATACTATGGAACCGTTTACCACCGATGAACTACTCTTCGAAATTACCCTTTAAATTTTCTCAATACATTGATATATTAAATTTAACCAAAGGTGTTATATCCAAAAACATTTTGCCATGTTTTATTAACGAACTCAACAAAATCCCCCGAATCGATTTGGATCCCGACACACGTTTCATAAAAAACGCTTTCGATTACAATTATTACATCAAGGAAAATGACATTTCCAAGATTTATGTAGTCGATGCCGAAACAAAAGTGTGTATTTCTCAAATTACAGTAGCAATGGTTGACGAAAATTGCATTAAACTGTTTGTAGACCTAGTATAAATTTTGGAACCACGGCACCTCGCCAATCTCGTCGGTCAAAGATCGCATAGTCAAAAAATATTTAAACGAATCTTCGAAAAACATGTACATTCCTATGAGTAAAAATATCACGAAAAACACAATAACGTTTTCAGTGTGCGGCAAAAAATAAATAATCAGAGCGCTCGCAAACCACAAATATGCCAAGTTTTTATGGCCACTTTCGTGGTACAAAATATTATTGTGTCGTTCTGTGTAGAGCATAAATTCTAGCCGCGAATACATAGAACTCGAAGCTAAAACGCTACCAACCAAAGTTACTTCGTCAAAATCGTCGATAACTTTACTGTCTTCAAAATTCAAAAGCTGACCATTGGAATTTACTTCCAACGCAGACACGTAATCTAAAATATAAAACAATGAATCGAACATGGCCTCGTCATCTTCCTCAATTAAATCATCAAAAAACTCCGGCAAAAACTCAATCATGTCCCGCGTTTCTCCCATCGACTCGAAATAAGCGCTCAAAAACGACCGCGACATATCGTCGGGAAATTCACGAGGAAACATGTTGCTATAGCCGAACGGGTCCCACAGCATGAGCACCAGATCAGTTATGGTTAGTAGAATTAACAGAATGCCTACCACCGATGCAGCTTTGATGGCAATTTTGGTCATGGCTTTAGCCACTGCAGAAATAGTTTTAATAGCGATACGGTTGAAAGAATGAACGATTGCCGCTTTGTAGGTTTCGCCCAATAATTTTACCGTTACTTGTTTAGACGAAGTTAGCAAAACTCTTTTCAATAATGGTATTAAAGAACTATTAATTTTCTTTAACATAGCTTTAAGTTGATCGAAAACGACATCGAAACCTATGCTAGTGAGAATGCCAAACAAAAACGCGTGATCCTCCAAAAACTGGGAAATTATCTCGTCCAATGTAGCGTTGTCAATTTCGTTAGTAAGCGGTACGAAACCGGATTCGCCAAGTTTAATACGTTCCTCAATTATTCGTTTCGAAATAGATTCTTTAGAAAAACCATTTTGAGCGCGGTAAATCAATTTAACGTTTTCGTTTATACCCAAATCTGACAACGTTTCAAAGTTGACCATGTCAATTTCTCTTTCCAAGTCCACAAAAGAATCTCGCTGATTTAGCCAAGAGTCTAGTACGTGAGCAGAGTTCGCGGCCGGTTTCGTGGGCAATTCGGGCGATGGACGCGTGTAGTCAAAGTGTCTCAGTTCGCTAAAAATATTATTAGCCATAAGCTTGAAAGTGACATAGATAGTATCGCCTAACACAAAACCTATTAAGCTTTCCCACCATCGCAACGAGCATCCGCCGTTGATGAGCTCGCGTCCAAATCGGCGGCAATAAGCCTCGTTGAATTCGCCTTTGAACATTTCGGGAAACAACGGATCATCACTGGGCGTTACATTGAAGCCCGGCACATCGTCGACACCCTGAATCAAATGTTCGTCGGTACGCAAGTACGGCGAATTCATGTACATTTTTGACAAAGTGTCTACCAAAATACATTTATTACCGTTTTCAATGTACCTCAATTCTGGCGATTGAACTTCGTTTTCGGCTCCTTCTCGTGTGGCCATTGCCCGGTCAAGATTGTAACATGCGGGCTGGGCATAACCGACGGCCACATCAGACGTTTGAGTATAACCAAAGGGTGTCGCGTAATCCGCCGGGCCGGTTTCATGAAACGGATAGCAAGACATACTCTCGCAACCGACTTTACTAAATTTTAGGCTAACGGCGATGGCGCGTTCGGCTAGTTTGGGCGGCACATAATAGTCGTCATTGTTGGCCGGTCGAATTTCATAGTCAACAAATATGTGGGGAAATTTGGTTCTCCAACGTGGTATGAAATTCAGGCGGTGCATGTGTGTCGCATAGCGAGAAGCGTTGGTCAAATCTAGAATCGTTAAACTTGTCATAGTAATTTTTACTTATGATTTCGTGTATAAACGAAACTAGAATTTGAGTAAAATCTTACTCATATTCAAGTTTCGTTGATATATATTTTTACTAGTCTTTTGTGAAACAACGAAATTAGAATATGAGTAAGATCCTACTCATATCCAAGTTTCGTTTATTTCCATACAATGAAATTTAGATTCGAGTAAGATCCTACCCCAATTCAGATTTCGATGGTTAGGTGTAAAAAAAACAAGAATTATTTACTCATACCATATTTTAATATACAATATACATCGCATTTTTCACAAGTATTGTCTTTTAGATGATTACATATAACAAAACATTTACCCAAACATTCATCGGTACAATAAAGTTCTCGCATGTACACGCTGCGACCTAATCGCGAAGTCGATTTTCTGCATTTCGTGCACACCATAGTTTGAACGGCATTATCAAAATACGGACTCAACATGGTTTTACTGATCGTGTTAATGAACAACATATCGATCAAAGTTTTGGGCAAAAAGCACTTTGAAATAAACGCCTCTAAACGGGTATCCAATTCGAGTACGGGCATAATTTTTTTTCCCTCAGTAATTTCGCCGCTAAGTTTGTTCAAACACAAATTAACCAAACTGCGAGGAGTCTTGTGCCATTCGTTTACTCTTCGAACGATATTTTCATAGACATCTTCTAATCGTTCATACTGCTTCAAGTCTACATTTATGTCATAGTTGTAACAGCGCAACAAACGCTCTTCGTTTTCCACCAAAACTGCGGGGCAAGTTTTATAGAACATTCGGCCCTTTAATTCTTTGTAGCGTTTGTTCAACAAATTATATGGTCGTATGTCGAAATTATCATCGTAACATTTGTCTACATGGGTGTAAAACGAACCCAGACGGGCAAAGTTCGTTTCATATTTCGTCCACAGGATGGATACAGAATGTGTCGAAGAGCAATATTTTTGTATTTTTTGAGAAAAACTCAACTTTTCATTGTAAGAATCGACACGGTCCAAATTTATAACTATTATAGACGTCATGTTCACACAATTTTAACATGATAACTGTCGAAACTTGGATATGGGTAAAATCTTACTCATATTCTAGTTTCGTTCAAGAGAATATATAAAGTTATTAATTAATTATGACTATCATTATTTTTAAAGCACTCATCATGGCACAAGTGAAAATCGGTCAATTCAAATTCGGCGAGGACCTGTTCAACCTGCGGTACGTGCTGGATCAAAATCAGCAAGTAAAATTTGTGGCCAAAGACATCGCGAATAAATTAAATTATTTAGATACTAAAAAAGCTGTGAAAGACCACGTTGACGAAAAATATAAATGTAAGTTCGAGCAGGGGGGGCGATTTGTCGCCCCTGCCTCCGACAGCGTAGCCAAGAGGGGTGACCCGTTGTATTTACAACCACATACTGTACTCATCAGTAAGGAAGGCGTCATCCAGCTTATTATGAAGAGCAAACTGCCTTACGCCGTGGAACTGCAAGCGTGGCTGTTGGAAGAAGTGATTCCGCAGGTGCTATGCACGGGCAAATACGACCCGGCTTTAAAACAGCAACAAGAGGAAAACAAGCAGCTGGTAACCAAACTAATAGCGACATTCACCGAACACACTAACGAGCTACAGAAACAACTAGTGAAAAAACAAGAGTTTATTGAGCATGTCGTCGCTATGAAAGACAAACAAATCGAAGCAAAGGACAAGCAGGTGATGCGCGTGATGACCGATCTAAACCGCATGTACACCGGATTCCAGGAAACCATGCAGCGCAAAGATGAGTTGTTGCAAACTAAAGATGAGCAAGTTAGCGAACTTGTTAACAAAATAATGGACCTATCGGATCGTGCGGTGCAGTACCCAGCGGACGAGCGCAAGCACCCGGTCCTGTGCGTGGCGCGCGACGGTACCACCTTTACGGCCATCGCCGGGCAAAAATCCTATGTGCACAACCAAAAACAAAAACGCAACATTGATGAGGACAACATCGTGGTCGAAACGAGGCGGCCCAATCCCACCGTGGACTGGAACAACGCGACGGAGCAAGTTTCGATGCCGGCCAAAAAAAGCAGACGGTCCATTAGTTTCGACTCGCCGGAGGACGCCCAGCAGTTCGAAGATCAGATCAAACAATTGATGAACAAATAAATTTTAAAATGAACCAAATTCTTTTATTTCATACAACGAAACTCGAATATGGGTAAGATTTTACTCTTAAATGTATAGCATCATATTCGAGTTTCGTTTTGAGCAAGTCTAAACTTGTCTAGATGATGTCATGTGGATGGGTGACCTAATGGGTCGGTCTGCTCAAATAGAAATTTCGCATGTGAGTATGTTTGAAACGATCTTTTGGGATGGGTGAGTAAATCTCATATGTAGTTTTGTATTTGTCAACATGATGTTATGTAGTGTGTAATCATACATCCGTTTGCGTAAACAACGGATGTATGAGATCATCGGTATGAGATCATCGAATGAATAAAAAAAATATTTCACAACAACAGTTTTTATTTCACAATCATTTCACAGCAATTTTCTTAAACTCTGTTAAATCATAATACAACAAGCTACCTAAAATCCGTTTCTTCATTTTAACACACAGTTTATAGTAATCAATCTTGGAAATGTCAATTTTGTTAATGAAATACCACGGATTTGTAAATCCACAGTACACACAGTAAAGAATCAAATTATTCTTGTACATCTTTCCGCGGCACTTTTCGCATTCCAACTTTATACAAGGCAAAGCAAAAAATGCGGTATAATCAAAATTAGTATTGTAAACTTTGCGATGCACCACATAATAGTATGTTAGATTCAAGTGAGTATGCCCGTTGACGAACTTCAGAATGTTACTTTCTTCGGGCACGACTCTGATCAAACCGTCCTCTATTTCATGCTTGCGAGCCGAAAACTCGTCGAGATTTTTTTGCAAAAAAAATCTGTTGGCCGATTCTGACACCAATGTTTTCTCCAGAGTGCAAATATCCACGCTTATAATTTGTTCGTTTGCCTTCTTGGCGTCCACTATAGATTTGAAAAATTCATACACGTTTTCGTGGGTTTCTTTCACGGTATTTTTAACAATTTTGTGGCTCAGATCCACGGGAAACAAATAGCGGTTGACGAAGCGAAGTTCGCACAACTTTTCCACCACATCGAGATACAATCGAGGAAACAGTTCGTAAAAATCGTAATTGGAATCGAATTGATTGCTCAAATAACAATCAAAACAGTTCATTTTGAACTTTTCCATAGCAGTTTGGGGTGTAGTAATATCGATACTTTTGTCGCTTCTCACATATAATTTTCTAGATTGAGACCCGGAAAACGGCTCGCGACATAGATCGCAAAAAGTTGACACCACCACGTCCCGATTGTTGTGGCGCAAACAATCCATGAGATTTTTGTTGGTCACAAAATATCTGCCCTGTATGTACTCGTTGGCGAATTGAAGGACGGAGCACATTAAATAATAGTTTTGGTTAGACAAAAATCGCCATCGCAAGTCCTTGCGAAGCGTGGCGCGCGGTGCGGGCACGGGAGTCGTCATTATTATGTGGAGGAAACTTCAACGAAATGTTGGCTTATATCATGTATTTTGAGTTCATAATTCGGTCTCATCTCAATTATTTAAATTCGTTTCCGGCGCACTTCCTTCTCCTACCACGCCGTTGGGGTCTATATAAGTTCTGTTGACAAAAATTCGATGACAGTTTTGTGGTATCCACAATGGATTACACCATCTCTAATAACACAATGGAAGCTGACAGCGAGTCCTCCACGCACTTTTGCAATATGGGCGAGGATTTTTTCAAAGAGGTAATACTAAAGTTTATATTTTCTAACCACTACAAACCCACTTTACCTTTGAATCCCAAACAACAACACGAACTCAGATTGGCAGCATTCGATATCGTTAACAAAATGTTTCTTCAAATGTACAACGAACCGATTCCGCCCCTGTTAATGTTTCGCGAAGTTGACGATGAAATTTTATTGTCGAAAGAGCGGTGTTCTCATCACTTGATCGAGACCATTGCCAAAGTTTGCGCCGCGATTAACCAACTCAAGAGCATGCCCAAATATAAACACCAAATTTTTATTTTCCTTCCTTACTTGAAACAGTTAAAAACTATTTTGTCTTGTTTTGTCAACGATTATTGCTGCGGAAAGATCGTTAATAAAACCCTAAAAAATTTAACCACACTGATAGACACCGGACAAAGTTCTCTTGAAACTATCAAAACACTTTTCGAGCGTGTGCAAGTGATGAACGTGTTTCGCGACGACATTCATTTATATCAATGTAATATTTGCCACGAAACTTCGGCTGAGAGCCATTTCCTTAAACCTAATGAATGTTGCGGCTACAAAATTTGCCAGGCGTGTTACGCGAACCTTTGGAAATTTAGCAATCTGTATCCCGTGTGTCCCGTGTGTAAACAGAGTTTTAAAAAATCCAGCAGCGGCAAATAGACGATTGTTTTGTGGTAGTTTTTATTATTAAGTATGAATTCGTTACACAGTCTAGAAGAGAGACAATATAAATTTTTGTTTCTAGCCAGTTACTTTAATCTCAACGATTATGATCACTTGCAAACGGACAGCAAACCGTTCATCGGCGAATATTTAAAAAACAACTTTGCCAATTTGAACGAGGAAAGTTTATTGTCATACATTGATTATTTACATTCCATCAACCTAAAGTTTTTAATCGCCGATCGTACCACGGACGTTTTCAAATATATCAAACCCCAGTTCAAGTTTAAATGTATCAAAAATAATATTGATATTTTGGAGTTTGACAACAAAACCTACATCCAACCTAACACGGCCATTTACGCCACCAATTTTTTTGTAAAAGATCCGAAAGATTTTCGATTGCTCATGTATCAGCAATTTTCTCAGGTGTTTAGCGACAGGCATTTTGTTAGTAACGGAGAAAACTATTGTTTAATGAACGGAAACGTCGGGTACATTTTCGAAGACTCGTACTTGGATTGGTGCGGCGCGCGCATGTGTAGCGTGCCAAGAATAGAGGAAACGTTTCATCCCTTTCGTTTGTATTTGATCGGCGACGAAATGGCCCACCATTTTATCGTTAATAACATCGCTTTTGATAAAAGCAACATGTGGATTTTTAAAAATTTTCATAAAGGTTTGCCACTATTTAAAACCAACTATCGGCTCATAAACTCTAAAAAATTTCAAACTAAAAAACCTAATGAACTTTTCAATGAAATGAGACAAGAGTTGGATCGTACCGAATACATTAAGTTTATCCAACGAGATTATATTTACGATGCCAACTTCCCCGAAGATTTGCTCGAGGAACTCAACGAGTACATGAGCAAAACAGCCATTTACAAGTTTATTACCAAATTTATAGAACCGCATGAACGTATGTCCAATTTTTACTCTGAAATTATTGTGGACAGATATGCGATAAATAAGTACCGCAAATTAAATATAAAAATTGAACCGTCCACGCTCTTTCCATCTTTGCGAATTAACGATCCTTCATATATTTTTGTACGACCCGACATAATTCAAATCAAAGGCACTCTGAACGCGTTTTACGTACCCAAAGAAAAACTGTTTGCTATTTTGGCGAGCAACAGTTTGTTCGGTTCCACCGAACTTTTGCATTTCGACATCAGATTAATTCCCTACAAACAGAGTTCGCCTCCAAAACGGTTGGAACTCGAAACTTTCATAGTAAACAAACAACAAAAACTATATTTGACTAAATTTATTTTCGGTAACAGGATCCCTGCATATCTTTTAATAAGAGGTGATTACGAAAGTTCATTTAAAACTTTGGATCATTTAAAAAATCCTTGGGTAGAGAACACGCTTCTTAAGTTGTTAAACACACCTATTCGCGCATAAAATGGAAGATTTGAGAAGTCCCGCGGGCGGTGTAGGTGGCCGTTTTGGCTCGTTTTTCAATCCTAGCGTCCTTATGACGGTGTTAATTTTTCTTGTGATTATCATTCTTATGATCATGCTTTTCCAGTCTAGTAGCAGCGGTGGTAACAACAGTAGTTCTCCCGCGCAAGGTGCCACAACAACATATACCAATCCTTTGAACGCAACAATGAGAGCCAATCCTTTTATTAACTCTCCCCAACGCCTATAATCTAAGTGAAATTCTTAAACTGTAACACGATGAAACGATTCAAATGTCAAACTCCTAAAGTTAGGACCGTCACTGAAATTATTCACAGTGACGAAAGGTTAAAAAAAGAATATGATATGGCAGAATTCGATGCCAAAAATCTCAACAGTCTGGAAGGGTTTAACGCTGTTCGCATTAAAATGATTATAGTCAAGTACATGGCCATGTTGAACACCCTAGATTTGAGTCAGCCTCTCTTAACGATTTTTCGCGATCGCAACGCCACTGTTGACATAACAAAAATTATTATCGGTTCTTTGGGGTATGTTCATAGGCGGGTGAATCCTTTAGTCACGAATTTCGAGGGCAGAATGGAAACGGTCATTATCGAAAATCCCCAACATAGCATCGCGGGTGAACCCATTTATTTTACGGAAACCGAAAAAGGCGACATTCGTTGCCATATTGACCGTGTCAGTATCGTTCGTATGTTGGAACGCCATTACGATATGGACGTTAAGATAAACGATTCGCCTCTCAACCAAAATAAACTAAAGTTAATGCAAGCGCTAACATTGAGCGGGAGTAGCAAGCGTAGAAGAAGCCACGATACTTCCCGATACGATTTGCAATTGACAGAAGTCGAGTGCACCCGCTACCTAACTCTACTGTTAATAATCGAGCATGCCTATTGTCACTATGTTATCCTTAAAACTTTCGACACGTACAATTATGTCAAATCGCTGTTAGACCATTCGTTGTTTGCTCACAAGTGCCGCCCCGGTGTCAACATGAACTTGTCAAGTTTACTTTTAAGTAAATTTAAATTTACTGTAGAAGAATTTGATCCGAACAAAAGTAGCAATAAAAATTTGGGCATACTAAACTATAAGTCGTAATGTGGCTTATGCTAGCCTTTTTTATCATAGTAAAACTGTTGGTATTTCACAAACTACAAAAAATGCACCTCGACATGCACGTTGCTAAAATTTGCCCTAACGGTTTCCACGGACTCGCCCCCGATCCTTTCGATTGCTCCAGCTACTATTTGTGTCCGCAATCGATTCAAATGTTTTGCACCGAAAATACCCAATTTGATCTGGAAACGAATTCGTGTGTGCCACAATCGTTTGAGAGCGGCTGTCTCGGTAGATTGTATAAAAATTTGTTAATTTAATCGCACACAATTCTTACCGGGTCGTTTTTGGTTTTAATATTTCGCCACATGTGTGTCACGATTTCAAAGTTTTTTTCATGTGAAATCGGCTTTGACAATATTGAAATAAAGTTAGCCGGCACATTTCCAGTAACCGCGATCAATTCTTTTTTGATGAATACATCTCGGCCGTTTTCGTCTACCAAGTTTACGCCTTTAATTTTTTTTGTCGAAAGTAACTCGGTTTTTCGTCGGACGGAGAATATGTGCCAAACTTGATCTTCCGTTTTCGATGTCATCACAACTAACGAAAGCGGCGTAGTAAAATATTCATTAAAAATTAGTTTCGAATAAATGTACGGCAAACGAACACAGCTCACAACGAAGCCGTCTTTAAACTTTTTCATGTCAATTTCGTTTAAAATATCTAAAACGACATGCACTTTTCGTTTTGGTGTATTTTCGTAGCCGCTAACCAATTTGGTACTAATAACTTCGTCTTTATCATCCTTTTCTGTGTCGTCACAAAACAAAACAATATTAAATCCGTTTATCGAAGTAGGACAGACGAATGTGATAATGCTGTTATCGTATGTGGGTCGGTATAAATGGACCTTCATTTTTTATTTGGTTTCAGTATACGATCAACGTTGCCGAGTTCGATCAACTACTTACAATGAAGAACCAAGCTAGCAACATGCCCTGCTTGGACAGAAACGTTTCACCTTATGTAAGTTTTGAAAAAACACCACACAGGGAAGACATTCGCAACGAGTCTCCTGAGCCGCCAAGCGCCGATATAGATACTTTAATGAAAACGGTCAACGAGATCGTGGAAACTATGGAGCAGGGAACTTCGGCGGCAACGACACCGATTAACGTCACACCGCCTACCATGCCGATTGGTAAAAAACAAATTTCTTTTAAAAGAAGCCATAGCAAAAGTGATGACATGAAAAGTAGCAAGAAAAAACGTCCCACTTTGACAGATTTGGCTAAAACTGATATTCATGAAGAAACAGAAAAAGAAAAGCAAAAAAAAAGTTCAGTCGCCAAGATTCGCGGACCGTACCGCAAACTAAAAATCGACAAGGCTGTGCCTATTACGCACCATATCAAGCAGGTGACTTTTGAACAGCAAAACCAAATTATTAACAGTGCCACCGACTTGATTAGGGAAAATACAAGCCAGATCAACGAGCTATACGGAAACCAATATACAGACGACCGCCGATTTACCGATTGTATCAGTAGTACGTATTACTACATGTTTATTGTGTGCGAAGACAATAACAATGGTAATGTTTACAAAATTCATTATGTGAATTGTGTGGCTAGGGTTACCGTAGAGTATGCTGCTCGTTACAGTTGCATTGATAATTATGTGATGGTAGTTTCCATTAACAATCACAGATTCATGATTTCGTATAATCTGTTGAAAAAATTAAACGTTAAAATCCCCAAATCGGAAGATTTTAACGAATCTTCCAAAAACAAAAATAAATGTCAGTTTAACGAAGTTAAAGATATCGATTTCATGGCCACATTGATTAACATGTTTCACTTAGATATTTGTTATGTTCAGTCGAACATGATGTTAATGTTGGCGGCGCTAGGTCCGAGCAAAGCGCCTTTCATAGCCGACCGGTTGTATTACATGATCAACCAGTCTGTAATATTTAATTTACCCATCAATTTGGCCATTAAAGAGAGTCAGTCTACGGAAAATGTGGACGATATTTCTGCCTATGTGCAGGAAATTATGAAGTATTCAGCAAAGGCCAAGTTTGAAACTCTTAAACATGGCGAAATCGGTATGGACAAGATTGTAAAACATGTCGACATGTGGTTTAACAACAAAAAAGATAAAACCTGGCCTTTCTTTTTTACTTACAAATACGGTAGCGTAGTGAGAATTTTTTACAACAAAAACGATGATAGTTTCAACAAATTGTTGAAGGTTAAAAACCGAAAGGAAAACAATGGTGTTAATTTGATTGAGACATATTTAAATTCTAGCGTAAATTCTGACACTTCCGAAAATTTCATTTTGATTAACGTGAAAGCCGACGAGCGCATTACGATTATAAAAAAGGGAAAAAAATATGTCTGGATCAGCACCGTGTGCAAGGAAATTAACGTTTTAGACATTATTTCAAAGTTTAGACGCTTCAGGCACCATATTTTCGATGTAAGCTGCGTTGCTCGCAAAGAGCTCAATAATACGCACAACGCCATGATCACTTTGGCCAGTTTCTATGTAAAGAATGTTATCAGTAGCAAACAGGCCGAGGAGATTGCCTCGCAAAAGTTTAACGTCAAATACAGGTCTAAAAATTATGAGTAAATATTGTGTAAATAGTTTAAGCCTTTTTATTATAGTTTTAAGCCTTTTTATATTACCCTATGAGATTATTATGTGTTTATTTTAAATAAATTTTTTAAAAATAAATTTTTGTTTATCCTTCATCCTCATCACCCACATCCATACTTTGAATGTTTGCCTGGTCGACATCATCGGCAACATTGTGGCCGTAATAGGTTAGCAATTCTTTGAGACTGGAAAAGCCGGTCAAATCTCTAAGTAGAATTTGAGCATTCGCTTTCCAAAACTCCAAGCTCAGATAGGTGTAGTACAGCTCAATAAAACTAATGATGCCCATTTCGCTGTTGAGAAATTCAATGTATTCTTCATAATTTTCCTTTTCGTTTTTAGTAAAAATATACTTTTTGTGAAAATCACAGCGGGTCTGATCATTGCGATGGTAACATAGTCGACAGTGTTCAAACTTCTCGGTCTGTAACGCGTTTAGATAGTCTCTCGTCTCGTCGGACACTTCTAAAACGACTTTTATATCGAAATTATCATCCTTTTTCCTTTGCAACAACAAACCGTGCGCCCCCAAATACTGAACCACATCGCCCAACTTAGAGTCGGGATTGACGGGACAGTCTTTGGTTATGGTAGCGGTCAGCTTTTTAAGATCGCGCCTAAAATCATTCATTCTTATAAGAGTGAGAATTCATAATGCAGATTTTTGTAAAAACTCTAACGGGTAAAACTATTACCTTAGAAGTGGAATCAGCGGACACGATAGAAATTATCAAACAAAAAATTGCCGAAAAAGAAGGCATTCCGCCCGACCAGCAGAGATTAATTTTTGCCGGCAAGCAATTGGAGGATAGTAGGACAATAAGTGATTACAACATACAAAAAGAATCTACGCTACACTTGGTATTAAGACTCAGGGGTGGCTGAAAAAAATGGGTGGATAATATAAAGAAACAAAAAAAATTTTTTTTTACATTTTTATTTATTTCAAAATACATCTATTCGGACATTTGACTATCTTCAAAAGTGTCATTACTCATTACAAATTCTGTCTTTTTAACACGCTTGCTAGTGGTGTTGGATTTGACAGAGTCTTTTGTATTTTTCCTTTTTCGTACAGGGGCAGGAACGACGTCGGTTGGAGTTGATGAATGGCTCGTATTCCTCTTCTTGTACAGTACTTTGACAAAAGTTTTTAGAGGGTTTTGGATTTTTGAACTGTTCAATTTTTCATTGTTCACAGTGTACGCATAAAAGGGATCATCTATAGGAGCGTGTAAATTTTCAGAGTCCAGTGTGTGCGTAAGCAGACGATAAAATTGTTCATAAATAACATTTCGTTTCGCGTTGATAATTTCGTGTTTCTTGGTTTCTTCGTTTTCATTCGTAACTTCAATAGATTTGCCCAGTTTTTCCACAAAGTCGAACAGATTGATAAAATCTGCTGATTCTGTCTTCATCAAGTTCCACATGTCACAGCTGCTGGGATACCACTCTTTTTTGATGCTGTGATACCAACTATTAAAAAGAATATATTTATTGTTGCTGATGATCTTTTTGCTGCGTTTAATGTTCTTCTTTTCGTCCCCGTAAACCGGCACCACCTGCAGTACATAAGGCATTTTTTTCTTTTCGCACAAGCTAATGTTCTGTCCTAGAAAATCATTTTTAGTCTTGTTATACACGCTTTGTTCACTCTTGTTGATGATTTCTGCGAGTGTTTGAGGCGCCATTGTATCTTGAGGAGGATCGCTTGTATTCAGTGAATAAATCAGTGTTTAAATTAAACACGTTTTCTATACGTTTGTGGTGCGACACTAAACGCTTGCGATTCTGGCTACAGTCCGCAATTACAAAGGCGCTTTTTAGATTATCCCTTATATACTTCATTTGCGTTTCAAATGCCTCTTCGAAAATGTGAGCGCAAACTCCAGTGTAATCGCCTGAATGTTTTCTCTTGTTTATCACTTCTCTAACAAGCGCGTAAATTTCGCTGCGGGTCACGCAAGATTCATCAAATTTTTCAATTCCGTTTTTGCTAAAGCCGAAATCTTTTTGCGTTTTTGTTGACATGTCGGTGTTTGCCGCTGGTAAATGTCTAGTTTTAACGACAGCAAAAAAAATTCATTTAAAGAACCGTAATCAAATTTTTTTTTAATGGTTTTCACAAAGTCAAAAAATTCCAAATAATTGCTCGAATCGTAAGTGAAAAGTTGCTTTTCGTTCATGTACTGGAAATAATCATCGAGCGGCAGAATATATAGGTTGCGTTGAATTTCGTTGTTGTATCGGCGAGTTTCAATGTTAATTTTGTAATCGTTGGGACGAAACAGTCTCAGCTTGACACAAAAAGTGTTGGGCTCTTGCGGAATGCTTTGCAACGGACCGTTCAAAATGCCAATGTAAATGGCATACTTGTACGTCACACCACAGTCATTCCACTGTAGCACAAAAGGACTTTTGTAAACATGGGCGCTTTCGAAAAAAGTGCCCGTTTCTTCGATAAACTCTCTGATGGCAGTTTCGTAATCAAATATGTCCTGTCCGTCGCGCTTACCGCGCGGTATGGATATTTTTTCTAGGAATGTTATAGCGTTGTTGGTGTTGAAGGCGGCATTGTACGCGCGTCGAGCCCTCAAAAGCACGGCGCGGTTATGGTCCATTATCATGAAAAGACCGGCACACTTCATGCCTCAAGTAAATAATAACACTACTGTTATAAATATGACGTGTATTTTTATTATATATCAACATATTTAACTTATTATGAACAATTACATTTGGAACAGCTATGACATTGGCACAAACGTTTCATTTACAATTGAACAATTCGGTGACTGTAATGAGTGCCCGTTTTTATTCGTTTTCCCTTAATGAAATCGATTTTAATTTCATTGTTATTTTGCACAACTCTAGATTGAGCGGCCGATCTTTTTATAGAGTTCAATCTACGCGTGGACAATTCCAGCGGATTATACAACAAAGATTTTTCCAGCGTAAAGGAATTTAAGTGCCCACCATTGTGCCACTCCAACGAAATTATCATATTCAACAAGAAAATTATTTCAGAGTCTCTTTTTTCAAACACAAACTTATTTTGCAAACAATAATAGTACAGTTTTAAGGAATTATACAAATAAAACATGTAATTGTTAGTTTTATAATAATGATCCACATCGGTCACGAACAGCACATTGATGATCTTGTCCTGGATGAGTTGGCTAAACTTTAATAAATACTGCATGGAATTTTTGTCGTCGTCAATACAAATCGCACACACGTTTTTCAGATAGACGTTTTCGATCACCACAAATTGATTGTTAACAAAAAGTTCTTTGAGGTTTTCGTTGTAATCATTGGCAAGTTCGAGCACTATGTATTTTTTGGCACATCGGTGCCGCTTGATCACGTCCGAAATCGAAGCGATGGACGAGAGAAACTTTTCAAAATACTTATCGTTTTTCCAGCCTCTAGCGCCGATCGTTTTGCGCGGATCGTCGTTATTCTGATGTTTGAAATCCAAACCACTTTGTATAAGTTTGGTGGTGATGCGAATGCTCAATTGCTGACCCAAGGTATAATTATTTTCATATCCACGTTCCCACATAACGTTACAAGCGAACGCTATCAAATCGGCTACGTCGTTTTGCTTTAACATTTTATGCAATCTATCAAAGTCACCCTTGTACCAACGATCTCGCGCCATCAACTTTAGCATATTTTTATCGTGCTCGTTTAACGATTTTCGACTCACGTCAATGTATTGGTCGATTTGGTCGGGCTTGCTGCTCCTCAAGCACGCCATGTCGAACACAACTACTTTTCTGTGTAGAGTGATAAAATTTTCATCGTCAACATCAATACTTTCCCACAAAATATGCTTTGACGTTTTCGGTGGCATGCCTTGCAAATGTAACAAATAATTGCCGATGGGATCGGCAAGGTACTTGCAGCATTGCGGCAAACGTTGCGTGGAAGTTTTGTAGTAGATAGCAAACATTGTGACGCCCGCCATACAAATGATATCACATATCTTGTCTGCATATATATAGCCCGTCATTTCGATAAAAGTTTTAGTTTATGTTCAACACCAGCAGCATAATGAATAGCAAGTATCATTTCAATCCTGCGAAACTGGAGATTATGGCTAAAATGTCGTTGGACAACAACGTGGCAGTGTCTAAATTTTTGCCAAAACATCTTGCCATGCAATTTCAATTTTGCCTGAGGTGTAACATTACTACTGTAGAAAAAAGCTTTAATAGGATCTCCTATTGCTACGACTGCGGTTCCAAGTGCCAACACTGTGACAAGCCTGGCAGAGTTCGCCGGCTGGAAACGTTACACGGATGGACCACGGACATGGAGTCGTTGGAACGACATTTCAACATTCGATTGAAAGATGTCATGACGGTCCGCCGGCAGACTCACATGCAATATTACGATAATTACATATTTAATAATGTTGCATACAAAATTTTATGCTCAGATTGCAAGTACAATTTTTGCGCACTGTGCAAGGCAGAGATGCCCGAAAATGAAATGGTCAAGATCGGAGTAAGTTCCGGTACCCTGGAAATGACCCATGCGTTTTGCTGTAAAGAATGCGTGTTACATTGCAAGTGTGTAGAGTGTCGGCGTACCATTAGCTTAGAGAACGTTTTAGAGTTTGAAAATAGCACGGCCGAAATAATTTTTGGTGTGTATGGTTATTCTTACGTGTTGATTTGCTTTAACCACACTCATACAGTGACCGCTTGCAACGAGTGTACATCTTGGCAATTTAACAATGTATGTGAAACATGTGAAATTGAAGGAAACAATAGGGAGTACATACACAATCATCAAACGTTTAATAATCAAAACGTTACAGTTTGTGATGAGAACGAAGCAGTAATAAAAATGCTATGTCAAATCGATAATAGGCAAGTGCTTTTTAATTGTATTCAATCTGTGTCTGAACAATTATTCTATCCCATTTCTTTCGACACAGCCGTAGGGTACATAGAAAGAGGCATGCTTAACTTGGGTCGTAAGGAAGTGATAGAGCTGATTATTTACGCGATTGTTTCAAGAAAACGCGAGACTATAGTTAGCTTTTACGACCAAACAGGCATACCGTGGTATACGTATAACAAGGAAATTTGTCCCGATTGTGAAGATAGGGAAAACATGTGTGTCTCTCGTAATAAACCAAGGAATTTGTACCATACAGCTATGTATGATACTCAGTGCTGCAACACCACTAAATGTAAAGTGTATTCCGAAGATGACGATATTTCTGATGTGTTTTTAAATGAATATAATTATTGTGAAAAATGTTTAAAACCTTTGTTTGAAATTATGGATGTACAATTCGATTCAGATTTTGTTTGCACTAACCAAGAATCTCTTGAATATTGCTAAGTTTTTAATAAACCTGTTTAATAAACCTGTTATATATTCGAAATCTGCCTGTTATTTATTATTCCCTAACTCACCATGCCAAAGTTAGGATCTACGGTAACGATAATTGATCGTTGCGCTTTTGAAGAGCGATTAAATCATGTAAAGTTTTTTGTGCAACTAATGAATGTTGTAATATGTGAAATGATCGCTTCGCACGAAATCACCAAAGCAGAAGGCGCGTCGTTGTGTTTAGCCGACGATACGGCCGCCTGGATTTGCGGACGCATTTCCGATTGCAACTTTGTCACTTTCCGTGTGAAAAGTTTATCATTTAACAAAATACAAAGTAAAATATTAAAGAAAAAATACAACTTTGAAGAAACTTACGAACAGCAACTATTGGGAAACGAGTGGCAATACCTAATTTACATAAACCGCACGTTCAAACAGGTAGCTATCAAATTGATTGTGGTCAGAGAGGACGTGCCCATGATTTATTCGAACCCGTACATAAAACTTTCGTATTTCATCAAATTGCAAGATTTGAACGTGAGCACTCTGGACTGCGATTGTGGCAACGACAACGACGACCTACAGTGGTGTAACGAGATGGACCATTATCACCGTTCGCTTGCTGTACAAGCAGACTTGTGTGAGGATAATCACAACGAAAATATTATTAGTTTAGTGTGTTTTTGTAAAAATAAAGTATATTATTAAGTTAACAAGATGGTTATTATCTATTGGATTGTGTTAATAGCCGTGATCGTTTTTATTTTTTTGTATGTAACGTGGTCAGAAGAAGCGGCACCATCCCCTAGCCCGCCTCCTAGTCCTCCAATAATAATAATAAACAATGAACTAAAAGACTTTGAGTATTATTTCGTTGAAACTTTACCCGTATATTTTAGTCGCAAAGCTGAAAAAATTGCAAATCCCACTAGACAATGGAGTTCTAGTAGCGGTTTTGTCGGTTTAGACCCATGGACAAGTACTGTCGATTTTGGTACTTTTTGTCACACCATGATAGGTTATGCCGTTAACTTTGTGGACAAAAATAGTAACCAATATTACGATGAACAAATCGGTTATAATTTGTTGATGTGCCTTCGTCTTCTCTCTCATCATCTACCCGACACGCCGCCCGTTCAAAACGCGCCTTGGGGACCCGTGGCGGATTGGTATCACTTCTCAATTACCATGCCCGAAGTTTACATGACGGTAACTTGCGTTTTAAAAGATACAATTTATTATGAAACCGCCGCAGAGTTAACAAAAACTACGTTATCGAAGTACTTGCCCACCGCCACAACGTCTTTGGGATGGGTGAGAACGGCGGGCAACGCGATGCGCATGGGCGTGCCGTATGTTTATTCTCAATTGCTTAAAGGTTTCAACATTAGCCAAATACGAATGCAGGATTCTGTACAAGACGTGCTCAAAATTATTAGCTTTCCGTTTGTAACCGAAGGCAACGGATTGCACATAGACTCAATTTACATAGATCATATTGATGTGAGGGCTTACGGATATTTGATTAATTCCTTCTTTACATTCGGTTACTACATGTGGTTCTTTGGTTCAGATGTCATTAATCACTACGGGCTAACCAAATCGATATTGAACGTTTCCTCCCCAGAAGGAATAGTAAACCCGGCAGTCATGTCGAGACAGGGCACTATGTTTTCCAATGTCATTGGAAACTTTGTCGATTATAAAATCGCCGTTCATTCTGCCGATGTGAGTAAAGTGCTAACGAAATTAACAAACAAATATTACGGGTGCTGTGTGGGATACACTACCAAACTGGCATATTACGAAGCTGACCCGACCAACTTTAATCACGCACCTTTGTGGGCTATGAACCGCCGATTGTGGCGGCGAGATTATCCCATAATAAATTACACACCCGAAACGGTCGGTTTTGAATCTGGCGTGCTTTTGCAAGACTTGAGCGGCCGATGGCCCGTGCCGTCCACGACCACCTCGACACAATCGTTTAGACCTGTCATGGCCAAAACCGCCGTTGTGAAAAACGAAACTCTCGGCGCAATGTTAGGGTACGCAAAAATAAAAGAATTAAACGATTTAGAGTTTTATTCGTGTACGGTTTATTATGAAACGGGCATGGTACAACTTTATTACAACATGAAAATTCCAGTAGATACTTTGTCCATTAATCCTCGAATGGTTATTTTGACCAAACCTGTGATCGACCAATCGACCAACTGGAGTACATCAAACAGCTTCAACACTGCCACATTCAATGGCGTAACTTGCCACCATGTCAATATAATCAATTTAGCAGGTTTAGCAAACTACGTGTATAGACAAGTTAATGCCGTTGAAAATATTGAACAAATTATAGCAAGATCGGTTTTAGAAGAAGGCATGGGGATGTCATGTTACAAACTAACCGTGACCGAAACTAATGACAGCGTCACCGTTACTAACAATTCAAAATACAATAATTTCACTGTAAACATACCGAATGAGGGAATTTTTATATTTTATTTTCCATATTTAGCACTGTACGACGACAATCAGTTAATAATTTCAAACGCTGACGAGGACAATGAAATACCAAAAATGATAGTAGATAGTTTCATATACCAATTCGATATACAAGCCGATATGGAACCTTTCAATTGTGTCCTAATCAATGACTTTTACCGAATATTTACCGACGAATACAGTTTTATTTTTAATAAGCTCTCCTAAACATGAATATCAACAGGTACACTAGTTGTTTTTTGTGCAACGAAATTGTTTATAGTTTTCGTCAATACAGCAATAAATCCTCAGACTTCTTTTTTAACAAACACAGATCTGTTTGTAAAAGTTCTGTGTATTTTTGTTTAAAGTGCTACAGAGATCTTTACGTGAAAAAGGTTGTAAAACTAAAAAATTTTACAATCCCTAACCGTTTATTTAGTAAATAAAACACACAGAGAAAATTTTGTTTTTTTTTTATTGAATAATTGTATTGTTACAATGAAGCAAACTCAGCATGACGTTGTATTCGCCACTGTTATCGGGCAAATTAGAACTGCCCTTGACGCACTGCAAACTCTCCGTCCAACGGTTGCCTGCAAACGACTGGTTTATGGTCCATTGATCTAGCCGAGTTCCTTCCACTTTTTCGTGGCCGGTATGGTTGATTTTTATAAACTCTTTAAATATGTTGTCTGGCGTGTTATTGAAAAGCATGTACGGAATGTTGAAAATAGGATATCGCTTGGCGTTTGGATTGTTAAAATCGCCTCCTCTCACCACATATTTTACTTCGACGTCCTCGAAATTAGATTGGCGAGAAAGATACGAAATGGGCGACAAACATATTTGCGCGCAAGTCCCGTCTTCTGCGATCCACTCGGTCAAATCTTCGGCGCCGTTTATGACTCCTTTCTGGCCATGAATACCGCATATTTTGATGCCTTCCAGATCGTCGGTTGAAGTTATCGTAACCAACTTTATGTACACTGTATCGTTAAAAATAGTAATGATGGTGTCGATTTTATTGATCGCTTGGTTTTTTATTTGCCTCAGATACAGGTAAATCTTGAAAACGAAAAAGTTTTTGTTTTTGCACGCTTCAATTTTATATTTTTTACCGTCGTAAATCCAACCGATCTTTACGTTGGAAACAACGGTTCCCGCGATATGCAACAAATTTCCACCTTCCACCGCCACACAATTGTTTTCTGGCGGAGGTTTAATCAACTTTACTACGGGAGTTTCGTTTTTAGTGTAAATCATTTTTCCTTTTAGTTTGTTAATTTTATTATTGTATAGTCGAATGGGCAATTTGATGTCAGCGATGTAAGGATCCTCAGCCGTCATAAGTTTACTGTCACGCACAATCGTCCATAATTTGAACATTTTGTTGTTAAGTTTGATCTCCGGCTTGACAGCGACGCAATTGCCAAACGGTAGCGTGTCCAAAAAAGTTTCATCGGTTTCACTGCTGGACAAAATCGGCATGGCATTTTTCAAGTTAGTTAAAGATACAATCAATTTGGGCGTCGGAATGGTGCTGTAGATTTTTAAAAAATTATTGTAATAATATTGAACCAACTTTGACATGAGACAATCAACGCTGTCGTTTTCCATAATGATCCCCACGTCGGTAGTGCTGTTTAAGATAGAATGGCTGTTGTGGTATTCGTATGGAGTTAACAGCGTGGCGATAGTTGCCTGGTCGTTTACCTTCACTGTTCGTTTAATACAAATCATACCTTCGTGATGATTGATAAATAAAATTCCGTTGGACATTTTCATCTCCACGGGCGTCATGCCCCGTTTAAATTCGTAAAAAATTTTGTACAAATCTGCTCGCTGACAATAAAAAACGGTCGGACGATCGTTGAAAGTTAGCCACAAAGTTTTCGAATCCGCGTTAGAATTAAAAACATGGGTATCATTTTCGTCGATCATTTTAGTTATTAATTTTTGTTCAATTAATCTATTGAACTTTTCACGGACCATTTCATAATCCACGGTCGGTAGACGAACGTTGCGGCATAAGAAAAATTTTTTACCCGCCACCGTCATTTCGCCGTGGAAAAAACTGTCCACGAACTTGACAAAGTCATCTTTCTGCATCAACATGTCTTGGCGCATTGTGTCATTGGTGATCCTGACCACTTCGTTGCCGATGCGATATTTCAAGTGTGGCGGTGTTATTTCAATGTTATTGTTGCTACTATTGTCCTGATAATTTATGAAATTCTTTCTTTGCTTGCTAAAAGTTTTTGAAACGCTGTAAATCAGTTTTCCGTTGACGATAGTGTCCACGATTTTTTTGCTATCTTTGGGATACAAAATGGTTTGCATTTTCTTACGCTTTGTACTTTCACGGTTAAGTCCGTTGGATATCAAAGTTTTGTAATTATTTAGCACGGGACTGTAGATCAGCTCCAGCAGATAAACGTGTTTGTATATAATTTTGTTGGCCAAACTGTCGATGGAATAATTGATGCCGGCTTTCATGATTTTCTTGATTTGTTCCACCAGCGTCTTGCTCTGGCACGGTTCGTATTCGAAAATATCGTTGAGAGGTTTCCATTTTCCGCTGTTTTTTAAATAGATGCCGAGAATCGCGTTCAAATCCTCGGACACGACATAGTCACTTGCGTACACGTCTCTAGCGAATAAAACGTCCTCGTTGTTATCGTACACCAATTGTATGGCTCTATTGATTTTTTTCTCTTCGTCCACGTTACCGTAAAGAAACATGCGTTTGCAACTTTTGGAGTAGAGTTTGTCATAAAAATTGTGAATCAGAATATTGTTATTCATCATTATGTTGGGGAAACTGAGATTACGACCATCGATCATAAAAGTGCCATCGAAATTGCAACTTTTGTTTGTGTCGTTTTTTCTAAATATATGATCGAGCCAAGTGCCGAAAATCACCACGACACATTTGTGAAGCACACACCGGTTTAAATTGTCGACGGCGCAGCAAAAGTATGATTTTCTTTCCAGCAAAAATTTGATCGTGCAATGATTTACGTTCTTTTCGTTGCAATTCAGGTAAAACGACAAACCGTAATTGTTTTTTAAATTATCATATAAGTTGTTAAAATCGTTAATAACATCAGTCATAATGGAAACTAACAAAGCAACTGAATTTACCGAAGCGAATGATAACAATGACCCATCGGAACGTATGATATATACTAAAAGAATTCATGAGGGAAGAAAACGAAAATCATTGAATCCCATCACCACCAACCCCCGCAAAATTATTAGAGTTAGTAAAAGAAGATTTGAATATGAGAGCAATGAATTTCACCCTGATAATTTGAGATATGTGGATTTTTACACATTTTTAGATGTCGATTTTAAAAATTATAATCTAAATGATGTTTTAGCAAAAATTAAAAGTAAATATGAAACATTGAAAAATATACACTTTAATTCAGAATATTTCGAAGATACTTTAATTTTCATTAAATTTGTAAATCAAGTATTTAAAAATGAAGACGCGTTATCAGAATATAATACAACAACTATACCAGTTTTAAATTTTTTAGAACTTGTAAATAAAATAAATGAATTTAAAAATTTCCTCGATGTGGTCAACGAAAAAATTAATGGGTCGAACATTCAAATGGAAAATAGACTAAATTTAAAAATAGTACCACGAAACACTAAAACTAACAGAGCTTTGATCACATGGGATGTTCACCCGAATAACGTTAACAACGAAAACATCAATATTGAAAGTATTATTGCACATTTCAGTATTTATGGTGAAATAATAGGAGGGGTAATGTGTGAAAATAAACAAGGTTGTGCTGTTTTAGAATTTGCTTTATTGGATGATATGTTGAAAGCAATAAATAGGGAACAAATGTACCGTGTTTCAGACTACACGGAAAATGAAATAGTAAAAATTCGCTCAGACACAATAACCAACTTAAAAGAACAGTTACAAAATATACGATCCCAGATTGTTTGAATCGATTTCATAAGTATGCCTTTAATAATAGTCGTTATAACAAAAAATAAAAAATGGTTTAAAAGTACAAAACGGCGAGCCGTTAAACGATATACACAAAAGTCTCTATCAAAACTTGTAAAACGAAAATATATTGAATTCCGAGAAACTTGGATATGAGTAAAAATAAACTCGAATATAATTTTCGAATGGATCTCATTCAATGAAACTTGGATATGAGTAAAAATAAACTCATATCCAAGTTTCGATTTAAACCTGTCGAGACTTGTCGGCATAATTATGGTGCAATAATTAAATGATGTCACGACGGCGTCCAACAGGTTGTGACCGGTTAGATCGAAACTGGAATATGAGTAAAATCATACTCACATTGAAAAATATACACTTTAATTCAGAATATTTCGAAGATACTTTAATTTTCATTAAATTTGTAAATCAAGTATTTAAAAATGAAGACGCGTTATCAGAATATAATACAACAACTATACCAGTTTTAAATTTTTTAGAACTTGTAAATAAAATAAATGAATTTAAAAATTTCCTCGATGTGGTCAACGAAAAAATTAATGGGTCGAACATTCAAATGGAAAATAGACTAAATTTAAAAATAGTACCACGAAACACTAAAACTAACAGAGCTTTGATCACATGGGATGTTCACCCGAATAACGTTAACAACGAAAACATCAATATTGAAAGTATCATTGCACATTTCAGTATTTATGGTGAAATAATAGGAGGGGTAATGTGTGAAAATAAACAAGGTTGTGCTGTTTTAGAATTTGCTTTATTGGATGATATGTTGAAAGCAATAAATAGGGAACAAATGTACCGTGTTTCAGACTACACGGAAAATGAAATAGTAAAAATTCGCTCAGACACAATAACCAACTTAAAAGAACAGTTACAAAATATACGATCCCAGATTGTTTGAATCGATTTCATAAGTATGCCTTTAATAATAGTCGTTATAACAAAAAATAAAAAATGGTTTAAAAGTACAAAACGGCGAGCCGTTAAACGATATACACAAAAGTCTCTATCAAAACTTGTAAAACGAAAATATATTGAATTCTGAGAAACTTGGATATGAGTAAAAATAAACTCGAATATAATTTTCGAATGGATCTCATTCAATGAAACTTGGATATGAGTAAAAATAAACTCATATCCAAGTTTCGATTTAAACCTGTCGAGACTTGTCGGCATAATTATGGTGCAATAATTAAATGATGTCACGATGGCGTCCAACAGGTTGTGACCGGTTAGATCGAAACTGGAATATGAGTAAAATCATACTCATATTCCAGTTTCGTTTTCTTTATAAAAGAGTAGTAAAAAATTTATAAACAAACTTTGAATTTGAGTAAAATCCTACTCATATTCCAGTTTCGTTGTTTTATAAAATAGTAGTAAAAAATTTATAAACGAACTTTGATTTGGAGTAGGATCTTACTCATATTCCAGTTTCGTTTTCTTTATAAACGAACTTTGAATTGGAGTAAGATTTTACTCATATTCCAGTTTCGTATATACAAATAAAATTGAAACAACAGATTGTTTTCAAATGTAGTTTATTTGAAATAAAGGGAACAAACATCTTCGACAAAAAAGATCATAATTAAATTTCTTGGTTACTAATTCGCTATCACTAACACATGATTCACATTGCAAACATTGTTCGATATCAACTTTGTAAATTTTGTCGTGGGTTTCGTGTAAGGTCCATGCCATTTTTACTCGCGCGTTCCACACTGTGTAATCGTTTTCAGACACCTCATGCATGTTCATTTCCCAGTAACACACGCTCAGCAAACAGTACATTGAAAATTCCTTGTCCGTTTTTAGTATGCACACGGGAAACAGGCAATTTTTACACAGCTCCGTTCCACTGTTGTCGGTGAAACAATAATCGCAAACCGAAACGGCGCATGAAGATTGTCTCATAAACGGTTCTTTAAAGCTTGCCAACCGTTCATTACATTGGTCGAAAAATTGAGTTCTCACAGTGGGATCTGTAAGTTTTTTGTATAATTTTGAGTATTTAATAAAAGGCTCAATTAAATTCATGATTAACGTTTAACCCTGATGAGTTGTGAATAAGTAATAACATGCTATTGATAATTAATTTACAGGATAAATCTGATTTCCTGTTTAGATCGTTTATCAAACTGTGGAAAGATACGTTTGTCGAGTGCCAAATTTGTTACGAAAAAATCGAAAATGACGGCGAAGTCGCCATCACTGATAACGGCTCGATCAATTTAGAAAAAATGTTTCATTCAAAATGCATAACACGCTGGAGACTTGAAAACACTAGGGATCCTTTTAATCGCAACGTTAAGTTTTGGTTTAACTTTCCGCCTAAAAACCAAGCAGAGTGTAGCGCGCTGGTAAATCAGATTAAAAAATTCATTGGTGATAATGAAACCGACAAAAAATACGGCGCGGAATTTAAAAGAGTCAATGAAGAATCGTGTATAGATGTGGACATTGACTTTACTAATTTATTACATTATTGAAGTTCAACGTGGTCTTTTGCACAATTACAACCACTTTAATATGATCGATCATGTTTTCTTTCTTAACTTTTAACGTTTTAATATTGTTAACATCGTTGTCATTAGTGTATTTGTAATAAACATTATTATGTATTCGAGACAACACATGGTTAATTTTTTTCGTTAATATATTTTGATTGATGTGAACTTTTGATTGAAAACGTATGGGTACATTCATTTCCTCGTTCGGTTTAGTCACTTGCGCTTTCAAAAACCTTCTCAACATATTGATGGCTAAATTATTTACAACGCCACAAATTAACAACGAAATGTTGGTTTTGTTATGAGTGTAATTGACGTTAATTAAAATTGGCACGATATTTTCTATGTTATCCAGTAAACTATAGTAATATTTAAAAATTAGCTCACTCATTTTTGAATAATTTCGTGCGTTTATATCGTATTTGCCAAAATTAGACATGATATCTTCGTACAAAATTTTATTTGATAACTTCGATGTAAGTTTAAGTATCTTCTCTAATTTTACGAACAGTTCGAAGTTCAGGTATGTTATTAAAACGTTAAAGCCGTTTTGAATAGATTCTATATTTTCATGTACGATAGGCATCATTAACGGTTTGATTTGTATTATTTTTTGTCTATATTCTATTATTTCGGCTGCGGTTTCGTTTGGATCGGGGTTATTTTCGGAATCATTATTATTATCTTGATATATAATATCATAATCAGGTATATCTTTGTTCGCCATACTTAAATTATACTTAATAAACTATTATTTGATAATCAGTATAATCATTTATAGTTTCTTTTTCTTCAAAAGTGCGAATAACTTTTTCTTCCAAAACATCATAATAATGAATTGATCCGTCGGCATTATAGGACATGGTTTTAAAACGATTCAATTTATTAAATTCATTATAAGTAACTAGTTTTACTTTCGCATCATCAAATATGTCTTTTCTGTTATCGGAATCGGACGCCATAATAAAAAATATTCTAAAAAACAACCTCGAATTAATCGATGATACTTATATTATTTTAAATGTCATCGAAAACGACGAAAGTGGCAACGCCCAGATTCAACCAATGTGTATCGGAGAAATTAATTCCTTTCAAACCGATCAAATTACCTCAGACTCAATGTCCCTATCATCCTCTACGAGCGAATTGCCGACTGATCAAACCATGTGACCGATACGACCGAGAAGAAGATGCCGGCAAACACGTTACGGTTTTACAAGCTGCCACCGGCATCAATTATAACCTGCAGCCGTATTACATGTGCCTTTTAGACGACGCTGAACTGCGTGGCTACAGTATGAACGCAAACGAATTTTTTGGCCATGTCCAGTTGAACAAACTAGATAATGACGAAGAATTTTTTGGTATAGACGCTGCCGGAGAAAAAAATATGGGAACTATAAAAATGGTAATCAAAAGTATTATGGACAGTTTCGCAACATGTGAAAATTATTACATTTTGATGATTGACGAACTTCAACTCGATCTATTGTTTTCCATGTTCAGATCGATAATTTTACCTCAAAGAATGGTATCTATTCACAAAAACAATTTTGTGACCGACGATGTTTATTTTAAACTTTTCTCTGTACCCGTAACCGACGAATCGGATCAGTCGCAACAAATTTACCGCACCTTTTTGATGTATAACACCGTGTTAACTATGATACTCAAACAGCCTAATCCTTTCAATGACGCTAGAAAAAACATTTCGGTGATTTTCAGAAGTCTGGGGCGATGTCCGAACAATAAAGAAAGAGTTAAATGTTGCGATTTAGCCTATGGCGGTAACGCGCCGAATCATGTAATGTGTCCTCCCCGCGAAATGGTCAAAAGAGTTTTCCATTACGCTAAATGGTCGCGCACGCCCAATAACTACAAAAGATACTACGAAAAAATTACCAACAAAATTGATGATTCAAGAAACGATCAAAGTTCATTATCAAAATATGCGCTATTGACGTTAGATTGGTACAATTTTATAGAAGATTTTCGTACATACTTTGGAGTCTGATGGCAAAATGCCCAAAGTTGACCTATTTAAACGTTGCGCATGTCCTATTTTTATTTATTAAACGAATTGAACTTTTATCAGAAGGAGGATCGGACGCGAAAAAAATGGACATCAATTTTAAGCTGAAAGAAATAATAAACACTACCGTGGATAACAAATATGGAGAAAAAAGCACCAAAATTTCCTTGGCCGATTTTTATAAGAAACATCAAGAAGACATTGCGTGCGTCGGAAAAAGCACCACATACAACTGTACCGGCAAACGTGACTACGAAAAACACCTAAGCGACAAAAAATACAAATTCTAATGGACCGGCGAGTGGATGAAACTGACCAAGTGATTCGGTGTGCGCACTGCTTGTTTGTTGCTCCAATATCATTAAGTTATGAAGAATATCAATATTTACACAGGGTCTATAATAATTTGTTATGCCAAGATTGTTTTGCAAGTAACCCTTTCATGTCAACAACAGAATTGGATCCAACCACTAAGTTGGATGAAGTTTCTTGATGGATTGTTTTGTTTACCCAAATAGTTTGCCAGTTTTCCAATAAACAATTTAATAGAACTACACCAGCCTTTCATTATGTCTACCTTAACGCCGCTCCATATAAACGACGAACATTGCTTCGATAGTTTAGTTCGATTCGCAATGGCTACCAATATGAGCGCGCGCTTCTTGGAGTTTGAAGAAGTGTGTATTGATCTTAGAAATGTACACTTTAGTTTTGACCAGGACCATCAAAGTAACAATAAAACATTCATAATATTTATGAACGTGAAGCAAGCTTTTTATTCAAATTTCAAAATAAAAACGGATTTGTCACTTGAAACTTTGACATATTACATATATCAGCACTGTTTATGTACTGTGGAAGACACCGTTTTACCTATATTCCGGCGTTTCGACCAATTCATCTTTAATGAAAACGATAAATGTAAATCCATTATCATTCAGCTGCATCGGCGCGCCCGTGTCATTGTGGCTGAATGTATACGCGAGAACGAATATTACCATTCGGATGTGAGTGGTTACATTGATTTTGAAAACCGGCACACTAGATTACCGCTTTCGCTGAGCGAAGAGGAAAGATCGAAAATAAACCGAGAGGCACAATTAAAACTACTGGAAACGACCTAAAATCTTTGAGAAGAGGTCTCGATGTAATCTTCGTCATCGGCGTCGTCATCGGTTTCGTCGTCATCGTCGTCGTGGTAGTGACGTTGTGTGGTATTGGCGGTAGTGTTATTGTTAGCGTTAATGTCATCATCATATTCATATTCGTCCTCGTCTTCATCGTAATCTAAGTCATTGTAAGAAAGGTGACTAGCTGTGTGAGGCGCATTAGCAGGCGCACTGGTAGATTCATCCTCGCTGTCGTAAATGTCGTGCCGTACAATGTGTTCAGCGGCAACCTTAGGAATCCACATGTTATTTAATTTTACATAATTACGTTCAATAGCCTTTCTAGCCATGTGCAGTGCCACGTCTTCGTTGGCGCTATCTAATTTATGATATTTATTAAAAGTGTCTTTAAAAAGTTTTTTTGCTTTAGCGGGAAGCTCCTCTTGGTAGTATGCGTCGGTTAGATGAAACATTTTATTATACCCTTACAATTACTCTGTATCGTAGTCGTCGTTTTCAGTGTCGGTGGTGTCAAAATCGTTGTCATTTGTATAAGCTAACCATCTGTAATTTTTCTTGTAATATTTTCTTTTAACCGCCGACCACGCTATACTATTGGCCGACACTTTTGATTTGTAATTTTTGTATGCACGATTAAAAAATTTCATGTATATTCTACGACCATTGTAAGGAAGCCGTTTTACAGCGGAAGGTAACTCTGATATGCTGTCATACATTGTATTCTTAATAAATATATTAAAAAAAAATTGTTTCATTAATTTTATTTTTCCTAAACTACAAAAATCAGATCCATTTTATGAGATTCAACAAATAATCACAACCATTTAACGGGCATAAAATTATTGCCTGCAAGTCATATTAAATTAGATTCGGCACTGTCCAAACTATCAAACGACACACGTTTGGCCTTTACTAGCAATTCATGAATGTCGTTTTCGTTTTTAACAATATGCACAGTACTTCTGTCTGTTTTGCGAGCCATCACGCCGTTTTTACACAAACTTACATATTTGTAGTGAGGCAAAAGAGCGTCGCGAGTTTTCTTTAGGAGATTTTTGTGTTCCAAAGATGCGGCTACAAAAATTTTAACAGGCCCGTCATAATTTATGTTTAAATCGTAATTTTTGATTCTGATTTCACGCGACCGGTTTTGCCACTCTCGAGCTGTGGCAGCATCCGTCAAATGAACTACGATATGATTTTTTTCGTAATCGTTTTCAATTACTTGTTTCAAATCTAGTTTTAAAAGAGAGCATATTTTTTTAATGTAATTTAGCCGGATTTTTTTGTTAGTCAGGCGTTTGTCGTGAATACCGTAAATTTCCACGCTGTCGTTTAGATTGATTATTTCTAATTTCTTAAGTTTTTCATTTAATTCGTTAACATTGCGGTCAATTTCGCGTTTAATTAAAGTTTGTAAAATCGGCACGTTTATCAAGCTGTCGGCTTCCATGATGTTGGCAAGAATGTTTAAATCCGAAATACCCTCTTATTAATAATTCAAACTCTGTTGTTAATTTCAGTCGCTATTATGGAGCACGACGCGCAAACACCCAAAATCAATTTTGTTGAGAAAAGTCCCGCTGTTTTTTCGATACTCACCAATCCCGATCAAATTAAGAATGTGTTTTTCATTAAAATTAACAAATTTAGATCGTTTTTAAAAAATTTTTTGGCGGATCTAAAAAAGGTTAAAATAAACTTTTTCAACAGCCTGATCGAACAGTTGATATCCGTTTACACGGAAAACAATGTGAGAAACGAGCACACCGAAACTCTCGTTAAAATAATCAACGCTACAACTATTGTCATTACCGACTTGCCGTCCAATGTGTTTTTGAAAAAACTTAAAACTAACAAGTTTACAGACACAATCGATTATCTTATCCTTCCTAATTTCATATTGTGGGATCACAACTTTATAATTTTTTTGAACAAAGCTTTCAATTCGAAACACGAAAACGGTCTCGTGGACATTTCCGGAGCCATACAAAAGATTAAATTAACACACGGTGTTATCAAAGACCAATTGCAAAGCAAAAATGGTTACGCTGGCCAATTTTTATATTCTACTTTCCTAAACACGGCATCGTTCTACGCCAATGTCCAATGTTTAAACGGCGTCAACGAAATTATCCCTCCGAAAGCGAGCATTAAACGCTATTACGGCCGAGACGTTAAGAACGTTCGCGCATGGACCACTCGCCATCCCAACATTTCGCAATTGAGCACACAAATTTCAGATGTTCATCAACCAAAGGAATATACCGATTGGAACGTTAAAGTGGGCCTAGGAATATTCACCGGTGCAAACACAGATTGTGACGGTGACAAAAAAGTAATTACTTTTCTACCTCAACCGAATTCGTTAATCGACTTGGAATGTTTGTTGTACGGAGACCCGAGATACAATTTTATTTGTTTTGACAAGAATCGTCTGTCTTTTGTGTCTCAACAAATATATTATCTCTATAAAAACAGGAAAAATATAGAAGTGATTTTAAACACTATGCCTATGTTAAAAACGGTATGGCAAACATACGGCGATAGAAAGTTTTCCACCAAACTTGATATGCTGCTGCGGGATTGTGCATTATTATTGAGCTCAAATTGTAGTTATTTACTGTTTAAAGAATTAACAAACCTAATTGACGAAGAAGAAATGGTTTGCGGTGAAGAGGAAATATTCGAATTTAAAGGAATATTTAACGATGTGATCGACAGCGGAGCCAAAGGAAGTTACGACCTGATAAAAAGTACTAAAATTTACAAGGAAACCAGCTTCAAAGACATTGAAACCGTATCACAGCGAGCAATTAATAGTTTAAACAATTACATTTCGTCGCATAATCGTGTCAAGGTGGGCGGAGGCGATATTTACCACAATACCACAGTCTTACAAAACATTTATTTAAAAAACAATAAAATTTGTTACAAAAGTGACTTTAGACAAATTGGCGATATTTGTACCTTGCCTTCGGAATTTTTATTTCCCGAACATTTGCTTGACACCTTTCTATGTTAACGATGTAAACAGAACCCGAATGCTGCCGAACAAGCACCGCTACAACATTGATCGTTTCGATTACAGTTTCGTCCGGTCTCAACACACGCCATTGCATAGGCGACACATGCCATCAAAATTATTAATGTAGTTTTTAAACGCATTTTGATTAAAACTTTATATAAACTTATGTTTAAAACTCATAGTGTGTACGGTCGAAAACGACATCGGCACAATTGTAGAACCCTTCGCCGGCCGGATCGTTTCTTTGCCAACGCACATACAATAAAAATGGGTTTTTTCTGAAAGGAACGTTTACGGGTATAGTGTAGACAAAATCCGAATTACAAAGTTTGTCATGTGGATACGAAACTAAATTTGATCCGTCGCCGCCGATTAGTTCGAGATCGTCCCACAACAAGTGATGTTTACGATTCCAGTTTTCTTTAGTAATATAAACTTCAAAGTAACTCGGCTCATGAATTGCGGTGGGACAGAATCTAATCAAAGTTGGCGAAACGTCTTTTGATATAGTCGTAAGTTGCCAATTCGTAAAAGGTTCGTCCATACCGCTTTTGTCGCCAAAAACTGCGCGGCTATCATTGGCTCCCGCGCCGCATAGTGTGTGGGGAACGACATTGGTCTTGATATGTTCAATGTCTTTGTAATTAGCACCGGCCATCGCTGCATATTCATGATACTGTTGAAACATGTATTGAGCGATTTGAGCGGACGAACCTGACGATTCGCCTTTGTCTAAATATTTATTATAAACTTTTTTGTAAGCGTTCCTGCATCCTTCGTCTATTATACTATCGCCATTTTCGGGCCAATAAAAATTATTTTCTGAAAAACATATATATTGTCGAGCCACCGGATAGGACAAGTACCCATGCGACTGTACAAATCGAAACGAACTTAAAATTACTATAGCAAAATTAATTAAAATCATATTGTTTATTAATTATGCCAGACTTAATTGAAAACAACGAGGATGATTTTTATATTATATTGTATGAGTTTATTGAATTAATAATAAATAATATGGACGAGCCCGACACCGACAGCGGTTACGAGAGTACATAAAGAACGACAAGCCTCAGAGCCCATCATTTCGGTATCGATATGAGAGACGGTTACATACACGACACGATTCACGCCCCTGTTATACCAGAGGGTAAAGCCGTATTATATGTCGCTACTACAAAGACATATGCACAAAATGACCAATACAAAATTGGTTACACCACTCGGATTCACGGACGTTTATCTTCGTTCAACTGCGGCCGTGCCGAAGAAGACAAAATGTATTACTGTTTATACACTAGACCAATGTATAATTGTGCAAAGTTAAAAAGGCTGTTGAAAAAAAAATTGTCTTCACACCAATGTGGCGGAGATATGTTTAGGTTAAGTTTAGAAGAATTAGAGAATCTTATTGTTAAATGTTGTATTAAATTAAAAAAGGTATTACATTAATGTTTGGTTTTATTTTTATAGTTCATCCTTTACTACTCTCAATCCCTCATTCATAGCGTTAAGTATGTCGCCATCATCGGCGTCAATTTCCCAAGCAAACAAGCCTCCCAGACCGTGGTCTAAAACATATTTAGCTTTAGCCAAAACCGATTTGCCATCATCGAATGTAATCAACTCTCCGGTTTGCGGCTTGAACGCGTACGAGGCTTTGGCCACTTTATCGTAATTATATTCGTACGACGGCAAATCGGCTTTAATGTGACGATAATCTTTTACACCGTCCTCCCATGTGCCCGCAATGGGACCATCGGCGATTCCGGTAAAAGGATTTTGGTCGTTGTAATCTTTAACACCTCTCCATCCGCGACCGTACATCGCTACGCCCACAACGATCTTTTCGGGTAAAACTTTTTGTTTTAGCAAAGCGCGCACGGCAAAATCGGTGGTGTAGCGTTCGGTAGGGTTCCAAGTTGGAGCATAAAGATTAGTTTGATATCCAAGATCGTTTAAAGACCAAGCGCCTTTAAAATCGTAATTCATTAAAAAAATGTGATTCAGATATTTTTGAGCTTCTGTGTAATCGACCACGGCGATTTTGTCGTCGCCCGCACTGATGGCACTGGTTAATTCAAAAGTTTTGTTCATTTCATTTCCCAACTGATCTAGCATGTGGCGCAATTCTTTAAGCAACAAAGTATAGGTGACTTTGTCATTTTCAACATCGCCTACATTTGGGTTGGCCCCTTTGCCGCCAGGAAACTCCCAATCAATATCGACTCCATCGAAAAACTTCCAAGTCGACAAAAATTCCTTGACAGATTCTACGAACACGGCACGTTTTTTACCATCGTCAAAATGATAAAACGGATCCGAAAGTGTCCATCCGCCGATGGATGGCAAAACTTTTAGATGAGGATACGCTTTTTTAGCCGCCATTATTTGTCCAAAATTACCTTTGAAAGGTTCGTTCCATGCGGTAACTCCACGTTGCGGTTTTTGCACGGCCGCCCAAATGTCGTGGATAGTTACTTTGAAATCTTCCCGTCCGACGCAGGAACGTTGTAACGCTTCGAAACTGCCCGCGATAGTTTTAAGAGAATCGTTTATACCGTCACCGCCACAAATAGGGATGAATCCGTACAAAAGATGACTAACATTTGGTATAGGAACTTTATCTAGAGGAAATTTTCTATCATAAACGCCCCATTCAACAAAATATGCCGCGACAACTTTGTCGCTCATGGGTAAATTATCAAAGTTTTTGTTATTTTCCTGAAATTCATAAGTTAAAGGGTCCAAATGATCACCGTTAGTATCGGCCACAGTTACGGGCACCGTTTGGCTCGTTGAACAACCGTCAGCGTTGCATAAACGAACTTTCATTTCAAATTTTCCCCCCTTATCGGTTTGTATGGACGCTCGTTTGGGCTTGGCGTCTCCTCTCCACATTACAGTGTCATTGAAAACGATATAAGCAACATCGCCCGCTTCACCGTTCCACACGTTCCAACTGACCGGAATATCAATAGTTTTGTGAACACGAACTAATTTTTCGTAGGCGGTGGCGGCGGGGTCGGTTTCTACTAGAGAATATTTACGTTCTGCCCAATCGATTTGCGCTACTCCCGGTATAGCAGCGAAACTTTCTAAAACACAAAATGAAAATATCAAGTAAAATAATGACGCCATTTTTATATTGATCTTACATAAGCTATTTTAATTTAATGTATTTAAAGATGAAACTTTTATTGTTATTTTTATTAATCGCGGGCACTACTAGTGCAACTACTTTTGATTTATTAAAAGCGCCCGACTATTTCGAAAATTTTATTAGCAAATTCAATAAACACTACCCTGATACAGAAACCCGTAATTATCGTTTTAAAATTTTCAAACAAAATTTGGAGGAAATAAACAACAAAAATAAATTAAACAACTCTGCCGTTTACAGCATTAATAAATTTTCAGATTTGACCAAAAATGAAATTGTATCAAAATACACCGGTCTAACTTCGGCGGTAACCGCTCAAAATTTACAAGTCGGTTCAAATTTTTGCAAAATAGTTTATTTAGATTCTCCACCGGGCGATAATTTACCCATAAACTTTGATTGGCGCATAAGTAACAAAGTTACCTCTGTCAAAGACCAAGGGGCTTGTGGCAGCTGTTGGGCATTTTCAGCTTTGGGAAGTTTAGAATCGTTGTACGCGATTAAGCATAACAAACTCATAAACTTTTCAGAACAACAATTGATCGATTGTGACAGAGTCAACATGGGCTGCGACGGAGGTTTGATGCATGTTGCTTTCGAGCAAATAATGAATGTGGGCGGCGTGATGGAGGAAAACGATTATCCCTACAGAGGAGTAAAATCCAGATGCGCCGCTGATCCCAGTAAATTTGTTGTAAGTCTAAGTTCATGTTCGCGATACATATTTCAAAACGAAGACACTTTGAAAAATGTATTAATTACTCAGGGTCCTATTGCGATGGCCATTGATGCCACCAGTATTTCTACATACTCGAAAGGAATTATTAAATTCTGTGACAACTTTGGGGTGAATCATGCTGTATTGTTAGTGGGTTACGGAACCGAAAATAATGTAAATTACTGGACATTTAAAAATACATGGGGAACAGATTGGGGAGAGGATGGATATTTCCGTGTCAAAAGAAACATCAATTCTTGCGGTCTAGTAAACGAGTTAGCGTCCACTGCTTCTATCAAATAAAACAAGTGTTTACTGCAAATATACTTTAATTTTTTCCTTTATTTTTTCACGACATATACAACAATTTTTGCATCTTTCGGCGCATTGTTCACAAGTAGCAAAGTGGCCGCAGGGGCTAAAGCATATTTCTATCGACTTGTCGAAACATATCTTGCAAGTTTTGTTATCATCGTTTGGCGAAACTGTCGTTGACATCAATTTGTGTTCTTCTTTGAAATTTTGCAAGTCGGGATAAATTTTGTTTGACCAAATAGCTTTATCCTCCATGCAAATTTCGTCGTCAATTTCTTGCAGATCGGGCGCAGACGGCTGGGAACGTTTTACAAAGGAGCAATTTTTTGATGTGCTGTGAATAAACTCTACACTATCGTTTCGATTCAGCTTTACAATCACCAAACCGCAATCGACACATCTAACTTCGTTGTTACGGCCATAAAAAAAGAATCCATTTTGAGCTAAAAGTTTCGCTTCCATTTTCACTCGTTTACGCGCCGTCTTGAATTTAATAAACGATTCTTTCCTTAAAGTTTCGTTTCGCTTCATTAATCTGATCGATTCGGAACACAGCGAATAGGTATGATATTTCAAAAGTTTCGAATTCAATTTCGTAATTATGTATGAACAAAAAGCGCATTTAAAAGATTGGTCATCAAAATAAAATCCGTTGCGTGCCATAAATTTTTTGTCTTGATTCATCAAGTTGGTGATGTCTTTGAAAGTTGCCAGACGATTTTCGTAATCGGTGTAAAAATTTTTAGGAGGCGGCAAACTTTTTAGGAACATTCGTTCCATTTTGTAATATGTTTAATAATTTTTTAAGAGCGCGTTTTTGTTTCAAAGCAAATTTGACAGTGTTTAAAGTAAAATTTTTGTGTTCTTTTCTTTCTGTTAATCGATTTTTGCCAATCAAATATCGTTCAGAATTAGCGGCTCTCGAATGCAACGGTTTAAACACTGTACAAAATTCAAAGTTGGCTGCGAAATCTTTCAACAAAATCTTTGTTTCCGAATGAAACGTATCGAATATTTTCAGGACACAATCGCCGCCCGGTTTTAGCGAGTTGAGAATGATCTTACATTCTTGGGCCAGTAGCGGTAACGTCAAAATTTCTTGGTCGTTTTCGTTACCTCTGACATCCGTGGCTCCGTCCGCTACAACCAACTCACATTTTTTACCACACATTAGGTTTAACTCTAGTATAACAGATTCGTCAAAAATGTCACCGCTATTTTTGTAACCGTATATTTTTTTAAAGTTTTCATGATTATAATTATAATCAAGTTTTCCTCGCAAAGTTACACCGTAACCTTTGCAATCAAAATTTGTAGAATGAATGTAATTGGCAAACTGACCTGGTCCTCCGCATAAGTCCAAAAATACATTTACACGTTTGCATACATTAAACTTTTTGTCGATATCTTTGAGTTTGTTAAAGCACCTTTTTTTAACGACCCGTTTTTCCATAGCGTCGCGTGCGCGATGAATCGATTTATCATCGATGTTATCAAGACTATTTTTGAGGTCATTCAATATTAATCTAAGTTTCGATTCCATAATGGATATAATTAATTGATAACAAAATGATGATAATGCTGCATATAAAAGCGTACACAATTTGTATTAAAGTTATACCACTCGGTACAGCCGTAAACATAGGTCGATCCAGAGCCCTCCTCAAGGGTTCATTGGTGACTAACAACTGTTTATTTTTAATTTCGTATATCAACGGACGTTTATAATCAAAATTTTCAAAGTTTGCTTTGTTAATTCCAGTCCGAAAAGCTGAATATTTTGGGCTCGCTAGGAGTAAATCTATCATTAGATTTTTGTACGCCCGTTCACGAAACTCTGGCGAAACTTCGATTCTGTCAGCATTCAGGACACGCCACTTGACATCATCCAACATGTCTGATCACAAGAGAATATCTGCCGGGCTCGAAGATGTTCTGAACGACGTCGATATGGAGTACCAAGGGACAGAGCACCGTAATAAAAAAAGATCGTCTAGCAGTGGTAGATCATCGAAAAGATCTATCAAAGAAGACAACTACGAGCACGAATCGAAACGGTCCCGTAAGCGAAGGAGTGAAGAAGAAACTAAAGATATGGAACCGGAACAAACGACCTGTCCAAATAAACTTACCTATTGGGAAAAGAAAAGATTGTCGAATAAAACATCTTTAGCTTTGGCAACGCCCAGGATGTCCAGCGAATCGGTAACAGACCAGCCGTCTACTTCAAGCGGAGTACCACAAACAAAACTACGCACTGTAAGTGTGAATGGTGAGCTGATTAGCAAGTTGCCAATCTCAATTAATAACAAATCATACTACATATTAAAGTTTTTGTGCGACCAAGAAACGAAAGAGTTCTATGGCAGTTCTGTTCACTTCACTGAAATAGTTAAAAATAACATGTACAAATTAGAGGTGTCTCAAACACCCAGATTTTCGTACATTGAAAATTACCAGATTTTGAACAGTGTGAAACCCACAATGAAAGTGAAGAAAGCTCTAGATAACAGCGATTTTGACGATCAAGATGTTGTGTCGGTGATCGCTCGCATAGAAGGCATCTTTCAAAACTTTACAAATGAATCTTACAAACTGGTTTTGTCTGTAAACTATGGATCGCGCGCGGTACAAATTGAGTGTTCAAGTAATGGTCAATCTTTGATAGAGGCTCTAAAATGCGATGATGTCTTAACAATTAAAGATGTGTTGCATTTTTTCAATAATAACATTGGTCGCGAAATACAATTGATCAGAGTCAAATGTCAGCAAATCAATAACGATTTAAAAAATTTAGTACTGACAAACATATCGTCTATAGAATTGACCAACGAACGTCCCGTAATAATGGACGCGTCTGAGGACATCAAAGTTAACTTGAGCCGTAAGCAAAAATTGATTCATAGAGGCACTTTGCTTTCTTTAAACATTGAAGTGCGCCCGTTTAAAAAAAGTCAGAGAATTATTTTTGATTTTGAACTTAAAGAGGATCCAGGTCATACACAAAATGCGTCATTCTTTTGTAATGACAGCATGAACGAGGACGAAATTTCCATATTAATGATGAACTTAAATCATTTAAAGACCATGGTGAACGATATGGAAGTTCACATTTATGAGTTGGGGGACGGGAAAATGTCGACTATTTTAGGCATAACTTGTAAAGTTTTAAATTGTGATGACTATTATTGCATCGATTGTAAAGCGCCTGAAATGTAAAAAAATTTAATCAAGTTTTTTAATAAATATTATACACTATATTTTGTTTTATTCAACAGGTACATTGGCCGGATTGGTCTTTAATTTACGATAAGCCCGAGTTCTAGAATAGGTTTCTACTTCTTTATATATTTTATTAAACGTCGTTATAGTATCATAGGCAGTATCTTTAATAGGCTCAAATTCATTAATAAGTTCTTGCTGTTGTTGTTTAATTAAATTAATATTGTCGTTTATGAAATTTTTATATTCTAAATTTTCTTTAATAGTTTGTTCTAATTTATTTCTATCGTGTTGTTCGCTTTGAGCGTTTAATGAGGAAATGTTTGCTGGCGACAACGGCAACTGATCAGACAAAATATCTATTTTATATCCTAAGTTTTCAATTTCATGTTGTAGTCTGACAATATCCTGGTCCTTTGATGATTGCATCTCTGCATTTTGTACACCTGTCGGAACAGGAACAACTGCAAAGTTAAAACTGTTTGTCGGGTCTACAAATACATTGGGATCTGTTACAGGATAATTATCATCACGTCTTATTCTATTTGCATGTTCGACCTCAGAATTTAATTTTCTATTAATTGCTTTTAATTCTTCGTTTTCAAACTTTAAACTATTTATTTCTTCATTATAATTATTTGGTATCGACGGTAGCGCGGGCGGTGTATATGCCATTTCAATAGCTCGCATATCTGTTGATCGATCTTCGGAAGATTCTATAACTAAATTTTCTCTATTAGCTTCTATTTCTCTTAATTGTTCTGATAATACTCTATGTTGGTTAGTCATTGTAGCAATATTTTTCATCAAAATTGGAAATAAATTACTTTCTTGTATATTTTCTAATGGTATATTAAAGTAAGTAATTAATATATAACGCAGATTGTATAACATTTGATTATACGAAGTTGTAAGTTGGGATGTTTTGTCGAACTTGAATAGACTAATTTCTTTGCTTGAAATTTGTTGATTTAAGCTGGTTATTTGTTGATTCAAATTGTCTATTTTTTGCTCTTTTGATTTAAGTTCCAGTTCTTTCATTTGGAGATCACTCTTGTGTTTTTGTTTTAACCGTGTGATGTCATGTTCAAAAGTATTAATTTTATCTTTCTGATTTACAACCAACATATCATTTATAGAGCTGGTTGTAGTTTCTTTAATACTCTGATTTAATTTATTTAACTTCAAATCATTATTTTCTCTTTCTAAACTATCATTTTTAATTTGAAGTTTAGTGTTATTACTTTCTAGAGTATTATAATTCTTTGTAAGTTGATCATAACTAGATTTTAAACTGTTATATTTAATTTGAAGATTATTACTACCCATAGGTACTATCTGTGTTTGTAATTTAGTATAGTCTTTTTTAAGTTGATCGTACTTGGATTTGTAATCTTCATCGGGTTTTTTGTTTCTGTAATTTTCCAATTCTGTCAAGGCGCTTGTTGTCGATTCTGTTATTTTTATTATATCGCTTTGTAAAGATGTAAAAATGAATTTTTTATCAAACTTGAGAGCAATACTTTTAAAACTGTTTAATAATAAAACTATAAACGGACACAAATCCGGTCCCAATGGCATGATTATATCGGTTTTATTTTTAATGCACCTCAATAAAGCTTGAATATCGTTGTCGTCGTCTTTGTTCGTATTGACATTGTTACCGATTGGCTGAGAGCAAGAATCGTAAAATTGCTTTAATTTTTCAAACACTAAAACTAAATTAGAACTAACAAAATATTTAACGTTAATATTTTTTAGTAAATTAATAAAGATAGTAAAATTATTTACATTAGGAGTTAGATTGTTTGTTAATGCATTTGACAGCTCTGATTCTTCCCGCTCATCTAAAGCAATTGGTCGTGTATAGTTATTTTGAACAGGAAGTTGTTGTTGATTGTTATAATTTTGCGGTGGCATATTTTGTTGAGGAGGCATCATTTGTTGCGGCGGTTGCGGGCTACCAAAAGCGTCCATGCCACCTTGATTATAGTTGCTGTTATAATCGTACTTGTAATTGTAATTATGAGTAATCTGATTAGGAAATGTTATTGGTATAAGGGCTTCTTGAACCAATTCGGGTATCTGCAAATCTAATCTCATTTGCAGGTGTGGCCTGTGTTGGCATATAATAGATCTGACACGTTCGAGCATCTCGTCATTCGATCCTTGGGCTTGACATCGTCTGTTTAGAGCGCTTATTGTTTTCAATAAAGCCTTTGTTGTAGGGTCTTTGTTAAATTGTAGATACGACATGGCAGGAGATATTGCAATTAATCTTATGAAATGGAGAACGTTAGTTAACGGACTAGAACAGACCAAATACAGAAATTTTGCTATAACTACACAAGATGTGTTTCGAATTACTAAAATGATTTACCAAGATAATCATTTGATAGTATTTTTGACGGGCTACCTACTGCAGGAACCAGAGCGGTGTGTGCAATTTTATGTGAAAGGTAAATGTGACGTGTATTCATATAAAATGTGCTACGAAACACATGCCAATGACGAGTGTTATCGTAACTGTGTGCCTTACAAAACTATGGTGATGCCGGGGCTGAGGCAAGTTTACAACGACAAAATTAATGTCATTAAATACAGAAGAACTAATGATGAAAACAAATATTGTTTGGACAATTTTATGAGAGACATTAATAGAGTTCATATGCAAACAAACATGATGGAGGGTACATACGTGAGATTCACCAAAGAACAGGTATGTGTAAATCAGTGTGTTCAATGTGACATAGAAAACATTGAACAATTTGAAAAACTGATTCAAAAAGTTAATATAGAAACATTGAACAAAGAAATCGTTCCCGTTGTCGCGTGTTATGACATAGAGACAAACTCAAACGGACAAAGAAAGTCCAATCCTTTGATCGATAACATCATATCCGTTTCAATTGTTGTTCGTCGCAACGAGAAAAAAACTAATATTTGCCTGTATTACATAGAAGACGGCAATGATGACGATTTAATCTGTAATGATGTTTTCAATGAAGACGAAACGGTAAAAGCTGTTAAATTTCATAATGAATTGGCAATGATTGCAGCATTTTTTAAATTGTTGCCCCTCATAAATCCAGACTATGTACTGGATTACAACGGAGACTTTTTCGACTTGAAATACATTTACGACAGAATGAATGTATTGGCGGAGGAACTGGAAGACTCCACTAATTTAAAGCGAAAAATAATGCAAATTCAACGTTACGATTTAGAACCTGTTGACATTGAGCGTAGAGAACTGACGGATAAGTTTCAAAACAAAGCCGACAGTCATTATTTCACCTATTACATTCACATTGACCTGTACAGGTTTCTAACCATCGAAAAAAACGATGCCGAAAACTACCAACTCAACACTGTATCTGAACATTATTTAAAGATGAATAAAGTGGACATGGACAATACCAGGATGGTCAAATTGTATAGCGAAAATCGCATGGTAGAGATCATCAAATATAACGTTCAGGACTGTGTTTTACCCATAGAGTTGTTTCTAAAACTCGAAATTTTAGATTTCCTTTATACTCAGTGCGAATTATTATACTTGTGCACCGAAGACGCTTTAAGCAATATATCGCACAAAGTTAATGTGGTAAATTTCCATAAAGCTCTAAATAACACGCGCACGGACGAGTTTGGAAACGAACATCCGGACCCGTATTTTTTCAACAAATTTGATTTAAGCGTGACATCAGGCCGCGATAATTTGTACCGTACACCCGCGACCAATACCGGCAAAGGTAATAAAAGTTTGGTAGATTTGACTCGCCTGAAACGTACACCCGTTTCGGCTGAGCATTTGAAACATATCCCCCATGTAAAACTGTGTGCTCAAAAGCAGATATGCCTGTATAGGGGCGGTAAAGTGTTAAATCCCAAACCTGGTATGAAAAAGTGGGTCGCTATTTTAGATTTCAACTCACTGTACCCCACAATAATGATGTCTGAGGGAGTTTGCCTGTCTAATGTATTTTTGTGCGATGATGGCAACGTTTATTTGCACAAAAATATAAACGCTATCAATCCCAAGCTGTTACAACAGCTGCTAAATCTCAGAGTCATTTATAAAAACAAGCGCAACAATTATGTCAAAGACAGTTTCCAATACAATCTAAATGACCGTCTTCAAAATGCTGTCAAACTTATAGCAAATAGTATTTACGGTTACTTTGGCATATTTTTTAAACCGCTGGCCAACTACATCACCAAAATCGGTAGGGGAAAGTTAGGGGAAGCCATAAAAAGAATCGAAGCCAAAAGCAACGACAAAGAGATTCTTAGGAAATTTGAGCTTAGTAGAATCGAATTTAATGTCATTTATGGTGACACCGACTCCTCGTTTATTCAAGTCGATTTTGACGAGTCGGAAATTGAGGAGGAAAAGTGCCATGACACAATCAAAGACATAATTAGTAACTATGTCATGCGCGACTTGAACGCGTCGTGGTCGGGTTATAAAATGGGCCTCGAAAATATCATCAGCGACATGATTTTGCTAAAAAAGAAAAAGTATTGCTATATTAATAGCGAACGCGAAGTTAAATACAAAGGTTGGCTCGTGAAAAAAGATATGCCAATGTTTATGAGAATAACTTTTAGGGAAGTTGTGGATTCCTATTTGAATCGACACACGGTAGCGTGCGGCCTCAAACTCATGTATAAATTGATGCGAGCTCATTATGATAATTTTGACAAATCCGACAAACTTTTAGATTACAGTTTTAGTATGACCTATAACGAATCCACTGGCACGGGCGCGACCAAAAATAAAGACGGCACATCTCGCAAAAAAGTCATTACTGTGGCCAAAAAGTGCCGAGAGTTTTTGGTGGCGGCCGGTGCCGATTTCATACCGGGCAATGGCGATAGAATCCCTTATTTGCTCACCGACATTGACGGTAAAGTGACTGAAAAGGCCTTTCCTTTGAAATTGTTTCCAACCAGCGATAAAAACATCAGTTGGCTCAAACACATGAATATTTTGTGCACTTTTATGAATGAGCTAATTCAGGTATTTGGCGACGACCCGAGTTTCGAGTATTACTTCGTAAAGATCTACAATTATTATACTCAAAAACAAAAATATGATGTTAAACATCCCACTTTGGTTGAAACGATTGTAAAAAAAACCGCTTCAAAGGGCCAAAAAAATAGTTCTTCTGATGAAGACGACGAAGAATCGGGTACAGATAATGATGAGGCGGACGCGGGTGTTAAGCTTAAGCAGTTTAAGATGTATTGTAAAAAACCCAAAAATGTAAAACACTACAAAGGTTCGCTGTGCACCAAGTGTTTTAAAATTTGTTAATAAATAAAACCGTTTTCATAATTTTGTGTTTAATTTATTTTTATAATAGCATTATTAACCTCCACCACTAAAAACTCTGATAGATCCCTAATCATATAGTCGGATAGACATTTCTTTTTTACAAAAGACTTCAAAATATCTACAATATTTTGGTTAGAAAGAGTTTGGTAGTATAGATATTCCACTTGACTGTCGCTCAATTGTAAGCCGTTCGTGGCGTCCACAATTGTACATAAATCATATGTGTCGATACGTTTGTTAAGAAACATATCGATGACGTTCAGCAATTTGTCAAAAAACATTTCATCTCTCTCCAAATCATCATGGTAGTTTTTGATGAATGTGCTAACCGTGGCGATTTTAGTAGCTAGCGGCATGCCGTTGTAGACCACTTGAGAGAAGAAATTTTTAACAAAATCCATGATTAGTCTGTAGAACTTGTGTTCTTATTTAAAATTGCGTCGAACGCTTTTTCTAAATCTCTCTTCTTTTTTATACTCTTAGCTTTACCGGTTTGTAAATCGGCAGCCGTGGACTCTGGTTTAATGTAGTAGACCTGTAGCATTATTATAAATATAATGCAAACTAGAAACAGGAAAAATACAAAGTTAGAAAATCCTTCGTTTTTATCAAACAAAAATCCTAGCACGATTAACAACAAAAACGTTGTATAGATAAACATTTTGACACTTATAAAATAATTTCAGTATTAAAGTAACAAACTAAACACCTTATTAACTGCTGCTATCGCTGGGTGTGGTGGTAGATGTTTCTTGGTAAACATCGTCCTCGTCTTCTTCATCATCGGTTTCGTTTATGCCGACATTAAACTTATTAATATAGTGTTTGGTGCTGTTAAACGAATCATGGTTCATTAGTTTCGCCACTCGTTGCAATGGCACACCGCTGTTATACAGGTTACTACTCAAATAATGCCTGATCATATTGGAGCGTGGCCTATCCATATCTACGCCCGCCTCTTCAAAGAGTCTTTTAAAATCTTTAAACGGCGTCGATGTATTTTTTGAAATTTGCAAAATAGTAGGGTGCTTGATATAAATTTCCCTAGCCAACTCTAGCGGTTTTGTTTTAATAGTATTAAGTGAATTTGTCCTCGCATGTTTCCTTTTCAAGTTTATAGTAGTACGAATTTTGCCCTTTTTTATAAGGACGGTCAAATCTTCTACGGAAAGATGACGAGCTTCATTTATACGCATACCGGTTCCTAACATGATACAAAAAACTATAGCGCCCCTGATCAGGCCTCGGTCATGGGCGTAATCACCGTTAAGATACTTAATTTTTTTTTCTATACAATCTATAACATTGTCGATAATTTCCTTTAAAACGATATTTTTTTCTTTAGTTTTAATATTTTTAAGCTCTTTGTCACGAGGCAGCATAACCTGTTTGGGAATTTTGTATTCCGGTAGACCCATTGTGTTTGAATAAAAATTAATGGTCAGCTGTAAAGTTTCCTTGGTGACGGAACGCAATTCCAACATGCGACGGCAAAGTTCTTCGGGATCTATTAATGTTTTTTGATAGTATATTGAATCAAATTCCGTATTCAAATCGTGAGTGTCAATTTCGTCTAAATGATCGTAATCGATTAAACAAAAAATTAATTTAATTAATCTTGACTTGTAACTTTTTAAAGTTGTCGGCGCAAAAGGTTTTGGAAACATGTATTGGGACCATAAGCTATTATTTTTAACTTCATCGGGCGTGCAACGTTGTCTGTCTGTGGCCAATTCAAAAATTTCATCGAAACGATCGTGACTCTGAATTTTTGACTTCCAATTATTAAACGTGTGTTCGTTTCTTAACGATGACATCGCTTTATCCGACTTGATTATAAATGATTTTTGGCTGATTATGATCAGTGTAAATAATATTTCTTCTTAATATTACAAAATAATAAATTGCATACAAAAGTAGCATAACGCATACCAAAGAAACTAATCCGATAAACAAAATGTTTACAGAGTTAGACTTTTCAAAATCATTTTCATAATTGTTATAATTGGTCAATAATTTTTTTTCAGATTCATAGCTTACGTTCTCGTCAGTTATATCATTGAGAGCTAACTTTAGCGGTATATAGTCAACTTTGGTGCCTATACCCAACTTTTCGTAGGGAATATCAAAATTCATGTTATTGGATATCCGATTGATTATTTTGTTTATAACTTAACTGTTGAAATATAAAACGTCTCAGTGCTTCGTTTTGAAATGCCAACTCTGTCAAACTTTGTTGGCACGAAGTTTTATTTTGAGTAGATGATGCAGCAGCGCCCGCGGACGACGAAGTTCCCGTCATGAATGTCGCCGATTGAAACAAGGTAGGTTTTCGGCCTGCGCGAGTCGCCAAGTCGGCAACATATTGAAAAATGTCCGTGGAAGCGGCCAGCGGTGCCAAAAATCCAATGTTTTCTTTTAGGCTAACGATTCTAGCGCGATTTTTTTCGTTTAAAACATACAAATAGTATTCGCCACCGCCCGCGAAAATATCATCAATAACATTATTGATAATATCGTTAATCATATTGAGGCCGGCGTACTTGCGGCTTTCCACGGCGCCCTGAATGTTCATAGGAATGTTTGCACGCTGCAATAAAAGTGTCATGTAGCTATTTGTCAATTGCTGGCTAAATGGGAGCGGAATAGGCACATTTGTAGCCACGGCTTCCGCCACTTGATATTGGATCGCCAGTCCAAGTTGGCGAGCGGCTTGACTCACGCTGTTGGCATTAATATTTTCGGCGTTGTTGTTGTAAAATTTTTGTGCGTACGATGGAAGTACGCTGTAAACAAAAGATGGTTGGAAAATGTTATCCGTCACCACCGAATGACCGAGTTCTTTGCGCAGACGCGTGTAGTGTTTTATTAAATTCTCGTCGTTATCAAAACGTTTGACAACATTTACATTGATAGGATTGGAATCGATACAAATGTCGCGAATCGTGTTGACCAGCGCCAGCATCTGAGGCGTGAGCTGTGACATGTCGTTCGTGCGGTAATATCGAATGATTTTTCCTACATAGTCCACACATTTGTTAAGCCACGCTTCATTGCTTGTGGTCGTCATAGTTTTTAATTTTTGTTATGTGAGAGCAAAGTATAATTTGAGGATCGCTTATCATATAATTATTGTTAATGTACAAAAGAAACATAAAAAATATGACGAAAAAAATATTATAGTACGAAAATTTTAACATGTTTATCATAATAACAAACAAAGCTAAAGCAATAATTATAGTTTGCATACTTTTACGCTTGCACAGAATGGTTTCGCAATTTTTAAATGCCACGTTAAAATCGTTTTCGCCCTCAATAAAATTTTTAAGTTCGTTTTTGCAACATTCATTACACAAAACCATTACTGATATTACATGACCGTATGAATGTATCTGCTGAAAAGTGCGAGGCTGACTTCCCGGATGAAATTCGAAAGCATAATTATTATACATATTTACTTGCGCGTAATAATGAGCCAATATGGCGCCGCAACTTTTAGTGACTTTAACTTTACAAATTTTTATGATATTAACTAAATTCGTGTATTGGTTGTCATCAAAAAGTTCGGCGTTATTAATATCGCCGTTTTCTTTTATGTTTTGAAACTCGTCAAACAAATAATGAATCAACAGTTCGGGGTCGTATTTTATTCTATTAAACCTCATCAAGTTTTTGTCTCTTAGGCGGTCTAGCCAAACAGGATTCGTCGTTTTCGCTGTCGTTTTCACTGCCGGTTTCGGTACCGTAGGACTGTTCGGTGGATCGTCGTTGATCGGAGGAGTAGTCATCTTTAACAAGATTTTCAACAATCTTATTATTCATATATTTGATTTTAAATATACAACAATTTTTTTTCAGCACCAAAGGTTTTATATTAAACAAAATCGTGTTGAGGTTATTTTCTTCGTTGAAATTATGGCTTACAACATAATCACCGCAAGTTACGGTCTGATAATTAACTAAATTTTCTGTTAAAGGCGCTAACAATTGTGTGTCCAAAATTTTCAAATGGTACGCACCGACAGGTAGTTTTTTTAAGTGATATTCATCTTGAACAATAAAAGATAAAATATTTTCGTGGTCTGTTTTATCAAGACTGACATAACAAGCGATGTCCATAGTACCATTATTATTATGCGCCATTATATTGATGATAATGTTTTCTATTTTATACTTAATATTATACAATGATTTTAATGAAAACGAATTTAACAAAAGGCTTAGTGTTTTGCTGGAATATATGCGGCGTACCAATTCCGAATTACCTACGCCGGAACAACTAAGTTACGTATCTCACGTCGATCAAAACTTTTACACAATCACCCAATTCTCCACGAGCGATTTAAACATTACAAAGCAAACATTACACGATGATCGTATCGAAATTTTCGATTTCCTCTCTCAAAAATTTCAACCGATCCAACATAACGAAGAGGCGCGAGTAAAAGCTCATAGCAAATATCATTCTAAATTTATGGTGAGAGGAGACGACGGATGGATGGACGTTGACTGTCCATTAAACGAAATTTTTGATGAAAGTATCATGCGATGTGTTCCTACTCCAATATGTGACCAAAAATTGCCAGGCAATTATGGACTCACAGAAAATATGATCGATAAACTAGTTCTCAATCACGCTATTATTCGCGAAAACGTAGATGAAAATTTGTTCCATCCCACAATGTACTTGCAATGTTTTGATGGCGGATCTCACGCGGTCATGGAATGTCCAAACGACCACATTTTTAATGCCACTACTAAAGTTTGCGAGTTAATAAATGAATGCGAAAATCGTCCCGACGATTTCATACTTACACGATTTCCGGATTATTTAAATATTAACGAATATATGATATGCAAAAATGGCGAAACTAAGATCGTGTCGTGTCCGAACGGTAAAATTTTCGACCGAAATCAACTAAAATGTGTGTTAGGAAACGCGTGCGAAATTAACGGCGTCGGTTTCACTTATATTACAGACGAAATTGGCGATTCGCAGTTTTTTAAATGTATAAACGAATCCGAAGCCGAATTAGTTACGTGTATTAACAGAATTTTTCGAAATGACCAATACGAATGTACCGGCGATGTTCAATGTGCCAATTTCGTTAACGGTTCGGGTACGCAAATAAAAATGTTTGAAGACGATACTTGGATGTTTGCAAACGGAATTTTGATATGCGAAAATTTTCAAGTCCAAAAAAATATAGATTGCGATTCCACTAATTTATTGCAAGACAAATTGTTCAACGAAAAGTTCAAAGTCAATTTGAATATACCTGCAGAAATATTTGATACCGACTCGGGAATGTGCGTACCCTTCACAAAAAATTTAATCAAAATTAAGAATGAAATTTTCCCCATCGAAAATATTAATAATGATTACAACATAAATTTTGAAACAGCCATGGTGGGTCGAACCGACGCGGTGGAAACTTTACTCGAAACTTCCAGTTTGGAATCGTGCGTGTCATTGGCTCGCGATATCGGCGCGGTCGGAATCAATCCTTTAAATGGAGATTCGATAAATTGTTTCGGCGAAAACCTCTATGACATTTTCACCGGAGATAAACTTAACATTTGTGATGATAATCAGCTAGTGAAAAGTGTCGATCTAAACTACGATTTTTATTTTCAGCCAAAAACGTCCAAAGTAGGTCGAGACGATGATTATGAGCGTTTTTGTGGGCAAAAAACTACAAACGCTCAAAAAATCGTAGAAAACGACCTTTTTTCGGCCCGTATATTTGCCCATATACTACGATCTGATGTTTGCGGACTACTTATGGCACAAATATACCATTCATATACTACGATGAACCGAAAATATACTACAATATACGTTCAATATACGTACGATATCGAAAAGAGCCCGAAAAATATAGTAGTATATGAGTCAAATATACAAAATAAAAACGCTACGATGCAAAAACGAGGGTCGACAGCAAATGATATTTTTGTAAATCGAGAAATCGTTGATGGGGAAAACGATGTAATCAAACCGCTTTTCGACCCATTTGAGAGATCAGAAATTCAACCTATATTTAATCCATTTGATGTCGCGATTCGTCCGCCGTTATCGTGGTTTCCGGAGTCTGAGCCCGATATTGAACCTGAACCCGAACCCGAGCCTGAGCCTGTACCAGAATTGATCTTAGCCGACAAGTTATTGGATTATTCTTGTTTCTATTCATTGCCCACATACAAATTTAGTGAATGTGAAATTGCCAACGATTTTATCGTTGATAAAATTAAAGAACTTCGCAACAATATAACAGTCGATCCTGAATGTGAAAACGCGAGAGGATTGTCTAATGTGTTTAATTCATATGTGTATCTGGGTAATGGAATTGGATGCAAATGTCGCTACGATGATGAGAAAGGAATTATCGTTGAGAAAATTTTGGACGGACCCAGATATCTAAATGTTGAAACTCAATCTAACGACGGAGTAAAATATAATCAGTGGGTACATAAAAATGGAAACACATTTATTGCATGCCCCCAAGAACTTCTCAATGATAATTTCGATTGTGCCGTTCATTCCGACAAATTTTACTATCTAGAGGATTTGCATGAATAAATAACAAATTTCATTTAAACATTTATTATTTATAAACCAACATTTATTATTATAAAAACCGACCCCATTACAATAATCTTAAGATTAAAATAAACAAAAAAAAATACATGACTTAAATCTTATTACAATAAAGACTAAAATAAAAATTTATTTTGAAGTCGAAGGTTCGTTCGACGCAGCACGTCCCGACAACGCAATATTGGCTCGAGTAATACGCAAGTTAAGATCGGTTTGATATTTCAACATGTCTTTTTGAATCATTACTTTATGCTGTAGATCAGTTATTTCTTCCTTCATAAATTTTTTATACGATTGCAAAACATTCATTTCTTTTTTCATTGCAGCAATTTTCGTCTCGAGTTTTTTGAATTCCTCTTCTTTAGACTTGCACAACTGAAATTTTTCCATAGCAAGTCTCTTAAAAACAAAATCATATTGTTGTCTCGCCTTTAATTTTTTTTTCTTTTCCGTTTCTATAGATTCTTTAATTTTAGTTAATTCAAGTTTGTGCTTTATAATCTCACTTGTAAGCGAGTTAATTGTTTGTGATTTAAATTCATTCTTTTTAGTGTTGTTGTTGTTACTATCGATGATATTTTTATCGAACAGTTGTCGTGCTATAAGAGCAGTATTAAATTTAGGATCCAGAGGAATAATCACATTTTTGATATTTTTAAGGTCAATATCAATGGTAACATAATCTTGCATGTTAACGGAAAAGATGGTGCCCTTTTTAGACGATTGTCGGCAAGTGGGGCATTTTATTTCCGATAGGCGCTGTGTATTTACATTCATTAGTTTGTAAATACACGGCGCACAAAAATTATGACGGCACGGATCCAACCGTATCAGCGGCAAAATACACATGTTGTCCACACTTACGTCGTAATCGTCTTCCAAGAGATGGCTGCCGGTGCAAATATGGCAGCTAACACGAGCGATATACTTGGTATTCATTGTAAAAGCTTGTGTTTGATGCTTACGCAAAGGCAGATCACAATAAACTGATTTATGTATGAAATTGTTTTCTCTTTTATACTCATGTCACCACCGGTGCGGTCGCAGGTCGATTTACCGGAGCAGCAGGCATTTCCGCGTCTGTGTTAATACTTGTAGACGGAATTCTATCTGTACTGAATGTATTTGAATTGTAAAATTTACGAAACGGTTTCTCTTTGTTAGTGTACAACGTTTGTTTTCCTAAAATCAAAGGCACATCAATAGAATAATTGTCGTAACGTGCTAGAGATCTCTGTAAAGCGTTGGCGTTACCCTGAAATTTAAGAACATTTTCGACGCGCAAAATATTTTCGTTAACATTAACCCTGTGTTTAGGTTTAACGGGATTATACAATGTGACATCTGCAACTAAGCCGGTTGAATCAATTTTACACGTGGGACAGTTTCTGATACACAAAGTTTCCGTATCGATTTTTATAATCTCTGGAGCGACAGCTTTTTCTATTAAATTTTTAATAAAATTGGGCAGTTTAATAAAGTTTTGGTCGCCAATATCATTTTCATTTTCGTCCGCGTTGCTGTATAGTCGGTTATCGTATGCTACTCTTGAGCAAAAGACAGTAGGATCCGTCATATTGAGTATAGTCATAGTTTTGCTGTATACTTCCTCTACTATTTTGTATGTTTGCTCCCTATAATTTTCGTTGTACTGAAGTATCTTGCAAATGTTGTCCTGGTTAGTTTTGTCACCATAAATGATATATATTATTAGACGTTCTGCCAACGGTAAACCTTCCACATTAAGTACGGTTTCGTAGTTTTTGTGTGAAGGTATTAAAACACGCGCATTACCCATTTCTTTGTCTCCCACTAAACTTCTGCCTACTGTTCTGTAATAAATGTTATTGTCGGCATCGGGTATGGGCAAAACCATTTTTTCGATTTTAAAGTGCACGGATGAATGGTATTCGCAAATAAACCATCCGTCGTCGACGGACGAGTCAGACGAGCATTGATTCCCATACCGAAAACACGGGTCGAAAGATTGAACAGCACCAAATATACAATAATTTCTAAGTCGATTACTTGCGAGTCCTGCCGGTACCAGCGCCATTATGGAAACGATCGAAAAAGAAATCTCTTATTCAATAAATTTGAGTCAGGATTTGCTCTATTTAATTTTAGATTCTTATATTTCTAAAAAGTTTGATTTCGTGGGTAAATATTATGATTTTGTGGATACGAATAACGTGCGAACACGTTTGGGAGACAACGGAGCGGTGTCGTGTCAAACTAAAAATGTACAAAACATGGAAAAGTTTGTCTTCGCCGATCGAAACGTGATCATACCTTTTGTTACAAGAATCAGCAATGAAATCGAAGTTAACATTGAAAATATTAAACCTGAACTCGACAAAATTGTAGAGTGTCGCGTGTACACCACCAAAAAATACCCAAAAATCGAAATCAAGTTTGAACAGATATATTTCAACAGAAATTTAAGCGACCGCTTTGATTCATTGATGGCAAGCAAACAAATTGCTCTGCTAAATCTGCTTCAAAACCGAAACGAAAGCATCGTTAAACAATCGTATTTGGGGTCTGATGAAATTCTGGCCAATCTTCGCATAGAATACGAGTACGACGACGGGCCGAACATGGAAACAATTAATGCGATTGCCGAAATTGTCCGCGAAATGGACGCCATCAGTCACTACCAAAACATAAGTCCCCTAATACCGTACACGACGTTACAAAACAATATTATTTACAGAAAATTTGAAGGCGAAAAATTGATATACAATTTGGAGGACTTGACGAACGTGAAAAAATGGGCACTAAAGTTGGACGGTATCAGAGGTAAAGGATTTTTTACTCGCAACTTTTGCATAATATTTATGGATGATATGCAATTGTTTGCCGGCCATTTTCCCACACTGTTCGAAATAAATAATGTGGTGGCTTTTCAATGCGAACTTATAGATGACAAGTTATACATTACAGATTTGTTGCACGTGTTTAAGTATACGTACAATAATAAAAGCCAATACGAATGTTCGCACGACGGCTACAACATCGATCCCATCACTGCCATTAACACTATAAATTTTCTTAATGGCAAATATTCGAATACAAGTCTACACATTGAAAACTGCCACCAAAACAAACTAGAAATCAAATTTCAAAAATTTTACGATCCACCGCTTCCCACGAGTTTGGGTTACACTACAATCGCCACGGACGGTTTCGTTGTGTTGGATATCACTTTACGCTATGTAAAGTATAAACACGTCAAAACTATAGAATTGGAATACAATGGCAAGGAAAACTGCTTTGCTACTTTGGAGAAGCTATTGACGAATTATAAAATTAACAGCAATATCGAACTGATTCACAACAATATTTACGAAACTGTCATAGTAGATAACGTTATTACCGTAATTAAATTTAGACCCGATCGTTTAGTTCCACAAATGATTCATGATGAAACGAAAATGGAATCGTAATATTTAATAATCAAATTATAAAGCAACTTACGAAAATTGATACAATTGGACGAAAAGGGAGAAAACTGGTTTCAAGTCAATCCTTTCGCCGAAGTTTTAAAATATAATAATAATAATCCTAAAGCTATTACAACACGTTAGCAAAGAAAATCAAAAAATGTTTGAAGAAATGTGTCTACCCGCAGCGGGCAGACCGATGAATCATCACTCCATCCTGCGACGAAGTTTATCAATCGTGTTTGTAGCGAAATTTTCGCTGGCAGGATGAATCCATTCTAACATCTATAAAAACCTGACGCGCTGTTCGTAGCTCATTATATCGTCAGTCATTGTTTCGTAACAAGTAAATTTCGTTGCATTTTTTTTAAAAATGTCCATTTCTAAAATCGAATTTGTCAACGGACCGATCGAAGTTTTCACTGTGACTGACGAAAAGGGAGAAAACTGGTTTCAAGCAAATACGTTTGCAAAAACATTAAATTATAAAAATTGTCCCAACGCAGTTGCTAAATACGTTTCTGCTGAAAACCAATGTACTTATGACGTTTTTAAGACAAATAACGGGTCTCCTCAAATCGAGGAGACTCATGAATCATACTTGTCTATTCAACCCAAAACCAAGTTCATCAATCGAGCTGGCGTGTTCGAACTTTTATCTGCTAGCGAAATGCCCGCTGCGAAGAAATTCAAGCAGTGGAACAATAACGATTTGTTGCCGAAATTGTGCGAAGATGGAGAATATAACATGGCTAGAGACGCGCCTATTGAAATTAGCGAGGGAATGAACGCTATACATGTAGCTACCGATCAAGACGGAAGAGAAGCTGCTTGGATCGAGGAAGCCAGGAAGTTTCAAGCCATTATAAAAGCAAAGGAACATAAAATAGAAGAGCTGACAGTACAGTTAAAAGATTCAAACGACAAATTGATAACATTTGCTACTGGTTTAATTCAGGCCAACAATAATTTAAACGAAGCAAATAAAGGTTTGATTAAAGCTAATGAAACTATCGGTCTAATGGCCAACCGTATGGCCGATATTTCCCAGGACGTGATCGCCAAGCCGTCGGATCCGCAGCTGTTGCATTCGCTCGCCGTGTGCGCCATCGGAGGGGAGCAATATGCGTTTTTGAGACCGCAGAAGAGGAGTCTACAGAGAAGCTTGAAGAGTCTCGACGTACGTCCGGACGATATCGTCTACCAGAGTGACTACGTACCCAACGCAGTGAACGTTTTAAACAAAATCAAAGAGAATCTTCCGAAGGACAAGTTTAAAGCGCGTCACAACAGAATCACTCTGCTGGACGACTTGACTCGCGAGCAATTAATCGGCGTGATCGACTCTACACTAAGTTCCAGACAGGTGGCTATAGCCAAGCGCAAGGTTGAAGAAAAAAATAAATGATGTATTTAAATTTAATAAATATTCATAAACTTTATTTTTACTTTTTATTTTAATTACTACACTATATTCAACAATGATTTATAGTGTTCCCAGTCCATTTTTTCCAAGTTGGCAGGCGGCGAGATATTTCGCTGCACGAATCTGTAATCGTTAATGTGATTATGGAACGCCATGCTAGAGTATAGAGTGCCGTACTTCATGAGTATATTTTTAGTGACATTGGCAGGGACAATATCGTTTATTAATAGTATTTTGTTGCCAAACTTCTCCTTGTGCATGGCAATCTCTATACGTTCCAAAGCGACAATCATGAAACCTTTAACACTAAGATAATGGTCTCTGCACATGGGGCAGTTTAAAACGAAAAATAAATTATAATAAATGGCCTTTATTTGTCTGATGTGGTTTATAATTTGATCATGTTTAAGTTTGTCCCTGTTATTAACCATATCGTCTACCAAAAGACTGAGGAAATGAATAAAGTCCCAGATGCTGGTGAACGTGTAGGTATAATCGTTTGGTTGAAACGCCCTCAAATTTAACTCTACCATTTTTTCCTGGAAAATTACTTTCATGTGTTCTAAATCAAAGGCGGCGTCTAGTGAAAGGACCCATTTTTTTATTTCGTCAATCTCATACTTTTGTATGTCCTTGTATGTTATGATGCACGCCAACTCGTATAAATAAGTTAATTCGGTGCAAAGTATTTTGGTCAGCTGTTTCGACTTTGATGTTCGAATCCGATCCAAGTGCCGAAAAAGGTGCAGCAAAAAACTGTTTCTGTAACGCGACAATAACAGCGAGGGCGGAATCATAATGAAAACTATCAACTTATTTTTGCACGATATGCCCCCCGGCGTTCAAAATGACAAAGAGATCGACGAAAATGTAATATTTTTCGACGGAGTGATCGAATGTGTCGAGGACGATCACACCGACAAATATTGCGCGCTGGCAGAGTGTGAAAAAATCAATGCCTTACTAATGCAAAAGATCGTCGTGGATTTGCTGGAAAATAGTAACGGAAACTATTGCAAGAACCATGTACTCATCGACAGTTTGCTAATGTATAAGACGTATGTAGAATTGGTGGACGAATCGGCGTTTGGTGAAAATTTTCTGGAGTCGTGTGTCCTACACATTACTAGCATTTTTAAATTGTTTCGTGCTCAAAGCAGAATCGTAGTTCTTTTACCGTCCAACATTAATTGGAAACAAGATAATTTAAGTGCACTTTTGAAACATTTATTACAATTATCTTTAATTGAAATTGCCTAAAATGACGACGATAATTTTGATTGTAGTGGTACTGGTGGTTTTGTATTTTTTATATGTCAACAATAAATTACCTCTAAACTCTCTCAACGAATCATCGCCTAGCATTAACCAAAGTAGCGATTCGGTTCAATTAGATCCGGCCACCGGTCAGTACGCGGTAAAACTAAACAACAGTAAAATTAAATCGTTTAAAATTTTGCACGGCGACAATCATCTAAGTAGATTGTATGTGTCTGAACGGCCGTTAACCTACAACGAAATTATTGATGAAGGTAATCGTACCGCTGCAAACTCTTATGTGTTTGTAGGCACAGTATCTGACCCCGCCACTTTGGCGGGCGCAACTTCAACCAGTCGCACGACCTTAAATTTCAAAATTGAGCAGTTTAAGAATGTATTCCTAATTTTCAAAAACTTGGACTTTAACAAGATTAAAGAAAGCGTTAATATGACTAGATATGAATGCGAAGGCATGGTTTATTGCCTAATAGATCCAAACACAAGTACCGTCCCAGATTTGAGGGATGTTTCCTATCCTATCACCGTTTATACAACCAATGTCAATGCACAATTAAAACTAAAGGAGTGGGATTATGCAGAGGTGAATGAGGCGGGAACCCTATTCATTAAAAATGAAAAATCATTTAGAATTCAATAAACTTTATTACTCAACACATAAACTGAGGCCTAACCGTTACAAAATTTTTCTTGTCCCTAACCATGGAAATTTTTTCATAACACATTCCTTTTTCGTTGAAAAATTTGGCACATTTGTTTTTAAAATCGCCTTGAATCTTTTCTATTTTTTTAGGCAGACCCGCCTGGGTTTTACACTTGAGTTGCTCATGAATCATGGTCTCCACAATGGGGACCGCTAGCGCAATAAGTGCGTTTACAGTTTCCTCGGCAATGGGTTCTGCGTTCTCGTTTAATTTTACACTCATCACATAGAAAAGAGCCTTCATAATACTATTGTTAATGTCTAAACACTTTAAGTTGTGTAAATGCATGGGGTCGTTTTGAAGTATGTTCCTGTACAAAACATAGCCATCGTTGTTTCGCCTATACTTCAGTATGTGAGACAAAAACAATTGGGCCCCCTTTCGATGATTTTGAATCTGAGATCGAATCAATGGATATTTTTTTTCTTTAATGTGCGAATATACCGACCCGCTAAATTGAAGTTCTTCCACAAAAGTATGATCGGTGTAAACTATACCGAACCGATTGCGAACTCCGCGATCATAGTCGCTAATAAACAAAGGTTTATTGTTAATAATAAGCATTTTATAATTGGCTTCGTACTTTTGGCTACCCTGATACTTGCGGCATATTACATTACTTTTGGTGGAGTCGGAATTTGTTTTGAAAAAGGCATCGTTACACACCTTCATTTCGTTAATGACATAAAGTTGAGAAATCAACTTGTCGGCTTCAATGTCATTGGTTTGCTCTTTGGTCAACTTGTATTTGTCAGAATCATGTTTGTGTACCACAAACATGCTTTCCATGAGTTCAAAGAAACTCGATTTTCCCGATCCTGCCATACCGTTCAAGTAAATGCAAAACTTGTCATAATCTGAGGGGATAGCAAGACTGGTAGCGAAATAAATCATGACTCTGGAGTTGACATGATTGAAGCCCGTTATGTTTCTAAAGTGTATGTACGCCTTCACAATGTTGGCCAGGGCAATGTTAGAGTAATGTTTGAAATCAAATTTGTTTAATATCAACCTAATATAGAATCGGGCACACCAGTCGTTTAGGTTGTCTCCCTGCCTGGCAATTATCAATTTGTCCCACCAAACATTGTAGCGCTTTATGATGCGTTTAACAAATTTGTAATGTAAATAAAATTTTACAATAGTGTTGTCCTCCTCTTCGAAATCTTCATCGTTAACAAACGTGCTCTTTTTTTTATTTTCAACATGTGCTACTATATTTTTAATGAAAATATTTGGGTCATTCATTTCATCGATAATGTCAGTGATATAGCGCACATGTTTAAATTTTGTGTATAGAAAATCGATAATTTCCCTTCGATTTTCGTAAAAGTATGTTCCGTAATTATTAATCAAATCGGAACTTGACACAATCTTTAAAGTTTCAATATACAAATCAAAGTTGTACAATAGAGACCAAATTAACTCTATAATTTCTTTAGAGACACTAAAAATATTAAATATTACAGCCACCAAAAAAGCGTTACGATTGATTTCAAACGTTGTGTAGCAATCACATACGTTTTCAACGTTAATGACGCATTTTTTACATTTAAAATTTTTAACAATGTCCGATATTTTTTTTTCGTTTACATACATTGACAAAGGAATAAGTTCCTTGTCGTCATAGTTTAAAATCTCAAGAAATAGCTTGTGCAAATTAATTTTTTCACTCATCTCTTGATTAGCGTCATTGAGCGTCATGGCTATAGATATGTTACATTTAATAATTTTAATGTCTCTACATATTTTTGCTACATGATATATTCTGTATATGTCAACTTCGTATTGGCTGTTGTCTTCCATGTATTTAATAATTTTTTCCGACAAATATTCTGTTTTTTTACGTTTAGTAATGGCTCCTAACAAAGTGTTACCCAAAATAAACGGACAACTATCGTGATAGGAATTTGTGAGGACATTGAAGACACCTTCCTCAGTGAAATACATGTACTTCCAATTGTTGAATTTTATGGAAGACATGCTAGATTCGGGCTCTTTAGAAACGAATTTAAAAATTTTGTCATCTTTATGTACGGCGTTGTTATACTTGGTGCCGTTAAACATAATTATACAGTTTGAAGTCAGAATTTTTTTAAAGTAGCCTCGCGCCAAAAGACTGGGCGAAGCACCGCAATTTAGGGCATTATCTGCTGTGTACAACCATTGTTTGGAAGTATCTTTTTCTGATTTCAACTTTAAATAAATCATATAGTGTATAGTGTAAAAATTTGCCAAAGCAATTCGAGGGTCTTCCGACGGAAACACTGTCCATTGGTTACATAAACTTTGAAACACCTCTGGGGACAACTCAAAATAGGGTTCACACTTTGCAACGGCTCGCTCAAACAGAATATTATCGCAAAAAAAATCGTTGTTGAACAACACACGACACACGGTTTCTAAGCAATATCTAATGTTTGTCTTGTTAATTTCCACTTGATCAGACATGGAAATCAGTTTCCAAATTAAAATTAAAAGGTAATCGAAATTAACAAAGTCACTTTTGTCAAAATATTCCAACAAAACTGACGTCGTTTTTTTCAATTCGTTTTGCATTGTGTCTAACATTATATTTATGCAACCGTTTACTATTTCGTTAATATAATCTTTATGTTCAGAACTTGAGGTGATGTTGTAAACGATTTTTTTATATTCAATTTCTTTAGTGTCAACATAAGTTTCAAAGTCCCTATACAATCTTAGATTTTTCAAATCAATATTAATAATGTTTTTGTACTTTTCGGTTACAACACATTGACGCAAAGTAATGTCTTCTCTAATTACATCAAACAATTTTTTGTCGGAAAAAACCAAAACGGGCAGCACTTTACAATAACCTACAGTACCGTCCGCAGTGATTATTTTGAACAAATCGTCATTTTCTTCTACGAAATCACTATTGCCGTTTACAAATAAAAAACCTCTACCTTCTATATGTAACGACATTTCCACGTTGAGAAAAAATTCAGCGTTAAAAACAAACAAGTTTACATTGCCGAGTTTATGATTATGTATCAGAGGTATGTAGTCCCCAATGTCTATTCCGAAATTCATAAATATGTACTGACGCCAGCCAAAATAAGAAACGTCAATATTGGGCCAGTAATAATAGTCTTTTGATTTGACACACTTGTTCGCGTACTCGTTGTCACCGACACATCGCACAAATTTGCTAAAGTCCACTTTATTTTTGATAATTTCATAGTGTTTTTTGTTAACAAAAGGTTTCACTGTCACCATGAAATAGTTTCCTTTCACGTACCAATCGTGGGGATCTACAGAAACACTATCTTGACAATAATCCAACTTGCACTGAGTATTCTTATCCGACATTAACTCCAACAGTTTTTTAAAGTTACTGTTAGACTCTACAACCTTTGTTACTCCAGTTTTGGAGTTTTTTAACACCAGGTTGTCAACAGTATCAAAATTTTTCATAATATTTTCATCACTGGTTTCGGCGTGAAAAATGGTATCAAAAATATGGTCCACATTTCTAATCGGTTCGGTGTCCATTGTGTTAATGTTACTGTTAATGGTTGTGATTTTTCTTTTAAACCCTATAAATGTAGCTGCTGATAACGTTATAGCTAGTCATGCAGATAGTTTGCACTTTGGGTCTTACGTTGAAGTTTTCGATTTGAGTTTTAATAATAATGTAGAACGTTTGTTTCTCGTCAATCCTGAAAACATTGTGATTTATAATAATAACGGGTTAATATTTTATTATTTGGATTCGTCCAGCGTATTATGTCCTCACGAGTTTTCTTTGCTACGTCTTTCAAAATCCGACATCAAATCTATAAACGAAAGCGGTATATTTCCGACAGTTTGCACTAATGTTAACGCCCTTTCATTGCTCGAGCATTTTTTAACACTAAAAAATAATGTTCCCGATCATAGAATCGTGTTAGCTGTAAACGAAATTAGCTACACCATTTTAGATATAATAAATATGCTAATTTATTCGGGATACGTTTATTTAGAATAAACGTTTTCGTAGTTAATAATGTTGGACATAATTGTTTCGTGCCAATATTTCCAATCGTTTATAGGTTCTTTGCATCGTTTCAGATTAACGAAATAGTCGTAGGAATGATCGTTATAAATCAAATCGTCCACCAAAGTAATGGTTTTTATATAATTAACGCCGAGTTTACGAAGATACCAAAGCGGAATTCGTGGTGACTTTGGAATTCTTTCTCTGGACGAATAATCATTATCCAAATAAAAAGGTCTTTCTACATATACAATTTTCGATTTGGCATCAACTATGGTGCGCTTTGACGGTTCTGTCGATTTGTATCCGCCACACAGCACAATATCAAAGAAACCGTTCAATTCAGTTTCGTCCATAGAACGCGAGACGTGTTCTCGACTACCGTACGACCATAATATCAATATACAGCCAAATTTTTTTAATCCTTCCAAGCTTTCATAAACGAATTCGTCTCTGATTCTAACGCGGTCTTCGCTCGTGATCAAGGTTTCGTCCATGTCAAAAACTATAACATGCGGTATTTCCCACACATATTTTTGAGGCTCCATTTGGAAAACTTCCAAGTAATTTTGAACGTACCATTCTTTTAAAAAACTATACATGGGCTTGCGTTCATTTATAACATACACATGACCGAGTGATAACGTTTCAAAAGTCATTTTTAAATGTTCTCGTAATTCCTTCATATTATCACGACATTTAATCAGTCGGACTATGTAATTGTCGGTGTCACATTTGTTGCTTTTGTCAAAAGCAAACACTACATACTCGAAAACATTCAAATGTTTGAACCCGACAATGTTAAGGTCGGTATATTCGGCAATGACAAGAATATGCCGCTTCATCAAAGGATTCATGTGCCTCAAAACTGTCCAAACAGCGGATTGTTCCATTGTTACCACTTATTTTTAATGTTCAAAGAGTTTCGAAAAAATCAAAATTACGAAGAACTCATACAATTTCTCAACAAAAACTTTCCCGCTAATGTTAAAAATAAAACATTTGACTTTTTGAACACGGGACACATGTTTCATTCCCTGTACGCTTACGTGCCATCGATTAACAATCAACAAAAAGAACGAAAGCAAATTAGACTCTCTGAAGAATGTATTAAAAAATTATTTGAAAATACAAAGAATGACATCGAAATGTACCAAGAAATATTCGATTTGATGCATCAAACAAACTCTGAGTTCGAGTGTCCTTGCGAGCTGCTGATGAAGCGCCGCGAAGAAATCAAAGCGTACGTGGGCACGTTAGAGCAAAAGGTGTTTGATACTAAACCCGTTAAATTAAAGAAGGAACAAATCGATGCAATTATGAGCAAATACTCGTTGGATTGGAAGAGTATATTAATCAAAAAAAAGGGTAATGAAGTTACCAAAAAAGGTGTTAAAAAGAAACGTAAAATAAAAAAACGTACTATAATGAACGACGAACAAATTTATTTAAAAATTAATGTCGAAAACAAACTTGGATTAATAAACGGTATGTCTTTAAGCAAATGCAGTCACGAATATGTTGTCGAGGAAAGGCAGATGCGCGCCGGTGATGAAATTGTATCTTTTGTCAAGTTATGTCGATTGTGTGGTCGGCAAAAGTTTGTTTAATAACGACGATTTCGGTATCTGTATGTTCCGCTACTGCGACGACGAGATGATCGGCGACGTCCTCCGCTCCTGCGTCGTCCTCCACTCGAGCGACGCCTGCCTACAGAACGGCGGCGAGATGTACGTCTTCCACGAGGACGGCCCGGCCGCCGCCGACTTCCGCCGCTGCTAGTACTACTACGTCTTCTGCCTCCATAACTTCTGCGTCTACCGCCTGTCGATCTTCGTCTATAAACCATTATAATATATAATATATAGTAACCGAATTTTTATTTGCACCTTATAAAATCCATTTATTAATTGTACTTTTTACGTTTAGCTCCTTTGTCCGCTCCCAAATAGACATTCAAACTATCGGACATTTCTAAATGTTTTAACGTGAGCGCATCACTCTCTTTGGTGCACTCCATGATTGTGAAAATGTTGTTATAGTCATTCACATCGAATTTACAATTAGCAACCGCATAGTTTTCCATGCTGCTGTAAAATATACTGTTAGCGGCGTTGTAAAACATTCGAGGAATAGGAAAATCGGTAATCATATCAAACAGATCAGTAATAAATTCTGTATCATCGCAATATGGAATTTTACTGCTGTTTAAATCGTTGATACATTGGTTAGAATCGAGATTTTTAATAGACTCTGAACGCTCAATAATTAAATCGTGTAAAGTGCAGCGTTTGTCTTTGGTCAAGTTGGTGTTTTGAAGCACCATGATGCGAGGGAAGCGGGCCAACGGGTATTTCATCGTTTTTATCATTGTGCTTCTCAACAGCTTCACGGTGTTCATGTCAATTGTCGAACCGGGCATTTCCATTAACAAACGTAGTAAATTTACAATGTTGTACTGTTGATCAGGCGTCACGTAAGGAGAACATTCCACATCGTCGTCCGCCAGGAAGGTTCCTTCTAAATACGCATACAGCGGTCTATACTTTGGTATTCTTGATAAATATATCATGCAGCAAATCAGATCGCTAATTTTAAACTCCACAGACGTGGTACTAGTTAGTGTATAATACTGTAACAATCGCACGCATCGTTTTCTATATTCGTTCAAGTCTTCTATCAAATTATCAGACTTCTCTGTTTGTTGCCGTTGTGAAAACAAATTTAGCAAATTGCGCCGCTGCGGTGCCGATGGCGGTGTAGGCTCGGGCGCAGGCGCGAGTCCCGTAGCCAAAACGGTACTGTTAACATCGGCATTCAAAACAGACTGCCTGGCTACGGTTTCTAAGAAATCCATAAACTCGTTTAGGGAAATATTGAGTGTACTGTTGGAGTTGGATAAAAGCGGAAAAAATTTAGGCCATATTTCCATTTGCATCTGTCGATCGACTTTATTTTTGAGTGTTTCGATTTCCAAAAAAAGCATCACTCCGCTCATGTTGAATGATTTTAATGCTGTTTACTTAATAAGCCTTTTACTCGGTAATATTGTTATCGTAAATATCACTTAGCAACTTTAACGTGTTTATTGTATTGAGGGTATTCAATTGTATGACGCTTTGTTCGTTTTGAATGGCTGTTAAAATTAACTTTGCCGTTGCCGATCTTTTACTCAAATCTTTGAAAGAAACAACTTTGTTTTCTGACGTATCCGCCATAATTCGCTTGGCAGCAGTTTCTAACACGCTCAGACCGTCCAAAATGTTTTGTTCGTCAAGAGCGTCGCGCACTTGCTCGTCCTCCGAAGGATCGAATTTGCGATTGCGTTTTTTATTTTTATGTCCGCTCGTCAAATCGTCATCGGACGCCAAAGTTGCAATTAAATCTTCTGTACTAATCATTTTGTAACAACAAGGCAAAATCCTCGTCGATTTTATGCTTGTTCATTAATTTTCTTATATGTTCTTCGGTAACGATATACTGTTCAATATTTAAATCTTCTTTTATACTTGCCAACTTCATCATAAAAGTTTCAAATTTCTCATCGTTGTATTTGTTCATAATGAAGCGGCAGACGTTTCGAATTTCCATTTCGAACGGTGTCATTAATTTAAAATTATTAAAGCAAACATTGTTCTGGTAACCCAGTTTAGCGACCGACGCTTCCATGTATAATCTCAAGTAAAAACCGGTGAAAACGACCGAAGCAATTTTGTTTATCTTTGAATTTTTTATTTTAGTTTTCAAACACAATTGTAACATGAACTTTTTAAAAGGAGCAAACAGAGGAATATCTAGCGTATGTTGTCCTTTAAGAATGACCAATAACATTTCCAGATTTTTTACATTCATGCCCAAAGTGGCGCGTTTACATTCGTCAATCAACGGTTGTACAGTTTTTCGATCAGTAAACTCTGACGTTTTATCACACAAAATATTTCTGAGGAAATTGACAAAGCAATTTGTGATTAGATCCTCAGACGTGAAAAATTTATTTTCATAGTCGGTTTTTATTAAAACGAATAACAAAAGCGGTAATCCAAACATGGGCCTCAAAAATATGTCCCATCCTTGCTGTATGGTGGTGTCGAATTGTGTTATGGCGGCGGAAACGTAGTTGGATTTACAAGCGAGGCATGCTAGTTGATTTAGTGAACATATGTTACAAGCCGCGGCGATCGCCGAAATTGATGGGCCGACAATCGGTTTATAGTATTTTTGTAGATAATGCATTATTTCTTTGAAATCACGAACCTGGTTCATAAATTCGTGATTCAGAAACATGGTGAACAGTTTGGTCACATCGCTATTTTGTTGTTTCCTGTTCAAGTTTTGTTTAACAAAATCGATACACTTGCTAAACTCTGTGTGAAAAGTAAGTCCTTTGACAACGACATACTTTTTTTGGTCAAAGTACTTGGAAAATAGGAACGCTAACGTGTCGATTTCTTTTTCTAATAAATGAACATTTAAGTTTGCAATTTTAACATCGTTTTCATTTTTGCTGAATTTTAAACTGTAATCAATAACACCTTTCATTATATTATTTTTCTTTATGGACAGCACCGACCTTATTAGCGATATCAACGATGATGCCCGAGAATACCTTATTATCAATAATAAATCTATACCGCATAAAATAGTAAAAATCGGTGCTGACGGAGCGTGTCTTTTTAGAGCCATCGCTTTGTTCAAGTATGGCGATCAAAACATGCATTTAAGAGTTCGTGACGAAATCGTAAAGTATGTTATTGAAAACTGGGACGATTTTTCAAACTATACATGCGACGAAACTACAAACTGTTACAAATCTATATATGAATACGAAAAATTCATGTCACAACCGCAAACATATGGCAGCACGACTGAACTTTACGCCGCCAGTATCATTTATGCTATGCGATTTGAAGTTTATAAAGATCAAAAACTTTACGGTAATGGATTTGGAGACGATTCGTTTCAAATTAAAAGACTTAGGTTTTCCGGCCATAATATGCATGGCCATTTCGATGTATATTTGGCCGACGATAAATGTGATCCTATACGTGAGCCAGAAAAACAAAATGAAAATAAATTTGTTGATTTGATGTATAATTTGACTAAACGTTCGTACATGATTAGATTCAACACAGTTCAGGCCATTCACAAATATTTAGTTGAACAAGCGTTGCCTGAACAACAAATGGAATTGGCCGATATCGGGGATAAAATTATTTTTGCCCGAGATCAAATTAACACTGGTAGTTTTGAACTGTTAATATATGAACTTGCAAAAAGATTAAATGAATTATGGTCTGATTTTAGAAGATATTCAATTATAAATAATTTATCGACCAATACCGGCGCTACTGTTTTCGATAGCGTTAAATTGAGCGATGTTAGCGGCGAAACTAAACGCAAGGAAACACAAAAACGAAAAGTACGTCGACAAAATGATAAACGAGTTATTACAAAAAATAAAAACAAAAATAACACTTTTGAAACCGTATATTCCAATAATGTCCAACCAAGGGACACTGTATACATTCAACCAAGAAATTTGCCAAATTTAGAAACAATGTTAACAGTCTCAATTTCAGATAGAACAAAGATCGATATAACTATACCAGAACCACTAATGCCGTTCTATATACAAAGTATCGTGGCAAACATACCCACCACCACTACCTACCCATATATTACATATCCTACTAAAAATTTGTCGATTGTGCCCTCTCGTGAAAATTTCGAAACTAACATTAATATAATAAACGAAATTAATAAATCTATTTTGGATAACAACGTTCATTTTTATGAAATGTTGGAAGCGTTACTTTATTACGGAGGTAACGAAACCGGCCAAGTCAGGGTACTTTGGTTTATTAGTATCGCCGAGGATTATTTCAAAGCGTGCGCCGATCATTTTGATAATTTGAGAGAACAATTTGCTAACGAACCTGATGTTGATCGTTTCATCGTATTTATAATAAAATACAATTTTTTGTGGCATTACAGACAATTCATAAGTACATTAAACTCGTCCATGTTAACTTCGTATCAAAATCAGAAAATTGTACGGTTTTTGCAATTAACAAACGAATCGGTTCAGAAAAAATTTGACCGAATCAATATTAGATTCGATCTTAACGTCGTGTATGTCAACAAAGTTCCACAAGTGGTTCAATTGATGATAGGATGTTACTCCAAATTTGGTTAATGATTATCGTTTTCATAGCGGCCCTTGTTATGTTAACTTTGACAAAACTGAATAAGAAAATAATTAATGAAATTGTCTACTTCCAATACAATTTTATTCCGGAACCTCTAATTTCGTTGGTAAACGTTATACGATTAAAATAACTAAGTTAGTTTTACGATGGCTTGCCCTTTTAACATTACAGTTTGCATCAGCGAACGTTTCTTTTCTTTTCCCTACGAATATGTGGTACCACAATCTGATGTCGGCGGAGCGCCTGTTGACAATTTGGTAATTTACGTTCCAACCGAAGAAGACATACAGTACATTAATACGAGCCAATTAACAAACTTTAAATCCGTACTTGTGTACAGGCAAGAGTTTAACAATGTTCGTTCCGAAACACGTCTCGCAAAAAAGAATACTAGCGCGACGGTAGTTTACTGGAATCCAATTGTGCCGATCGAAGAAATCGGAGTTGGCGAAACTCGCGTTTTTAGTGTCCTTCTAACCAACGATTTGTTCTTTTGTAAAACAATTATTGTGGATCATAACACACCAATTTGTCCGATAGAATTTCGTTCGCGTATAGAATACAAAAAATTGTATCCGATAGGTGGAGAAGTGCCTTTGTTTTACCTCAAAGATTTACTTAACGATAACATCAATGATTTTTTGATTTGTTTCAACCGAGAGACCTCTATAATGGTTAAAATTTTAAACATTAAAAGAATCCTCTCTATTTTTGAGTATCGAAAAGTACCTGCGCGCTACGCTATAAATTTACCCGAACAGGAGGTGGACAATATCTACAACAAATTAACATGGGAAAGAACGCGAAGGCTGATGAAAGGAGATGTTAGTAATAAATGTGTTTATGTCAATCGGCATAGTTTGATGTATGTTAAAAATGCACAAGAAATGTTGGGAATCAAGAATTATTCGCGTTCAATTGTGGATTTTGTTAAAATATTTCAACCTCTCATTCAGCCCTACCAAATTGTGCCTGATATAATCATCAAACTAAACACGTTAGAACGTGCGAAACATGTCAGGTTGTATTGTAGAGGCGATAGTTTCGCTATAACTTCGTACGGTTCTGTGCCGGGCAACATGCCCGACGACAATGTCATCCATTTTAATTATACAGACACCAACAATAACAAGAATTTATTCGAAGTTAAGTCTAATTTGTTCGGCAATAACGCTGTTAATGATTTTACTGTCACAGCTGCTCGATACAACTACTTTTTTTAATAAGTTACAATGCGTCGTACTCAACAAACAACGATAACCGCTTTTGATCAATTACAACATTTGATCACAAGAAATCAATCATTTTACAAAGATTTTCTTTTGTTCATTTGTGTCCTAGTAGTCTTTATAATAATTTTACTCTTTATAATTTTATTATTTGTTCTAACTAAAAACGCATACGAGCGTAGGGAAACTTTTGCTAGTAATTTGGATTACAGGAATAGGATGTAAATGAAAATCAAACAGTTTATTAATACATTTATTTTGAATCTGTCGAACCGAAACCGCCACAATCCCTTTCGGTAGAAGACAAGGAATCGCTTTCCACCAAAGTCGGAGTGTAGTAACGCTCAAACACAATTTGCGCGACGCTTTGTCCTCGTTTGAACTGCCGCGTTTTTTTACCGTGATTGAAAAGCAGCACTTTGATTTCGCCTCTGTAGTCGTTGTCGATTATGCCGGCGCCCACAACTATTTTGTGATTTGCCGCCAGCCCGCTGCGACTTTCGATCCTTGCATACATATTTGTGGGCATTTCGATGGCCAGTCCCATGTTGACGAGGCAGCCGTCACGAGATTTAATAATAAAATCTGTTGGAGTTCTGAGATCGTAGCCCGCCGAACCTTCCGTCGCCTTTATCGGCATAAAAGCGTTTTTATTTTTTTTAACTTTACAAGAGGTGTTCATGGTTGTGAGTTCCGTTGAACAATAAACATGGTTTTTAGTAGTTTTTGTTTTTACAATAACTCGTGTCGCAATCGAAACTGCCACTGATCTTTTTTAAAACATCCATGTTCAAATTCAATTTTTTGATGACCGTTAACGATTTGACCTTTCTTTCGGTTTTCAAAGCACACGACGTGCACATGTAACAATCGCTAACAAAAATTAAAGCTAAAGGTCCTCTACCTCTGAGACATTTTGCGCCCATGTTGGTGCGGTGTTTTTTGAATCTTTTTTGTACATTCACTGAAATGCCTGTATAAATGCGATTTTGTACATTTCGAATCAAATATAAAGCCCATAGTTTGGTTGTCATTTTGTAAGGGCGAATCGCTAATATGTACGCGTATGTTACTTTTGTAATGCTGGGCGACGAATATGTCAAAGGGGCTATCGCGTTGGCTAAAAGTTTAATTGTGACCGGCACAAAACATGAACTAGTTTGTATGGTCACGAACGATGTTAGCAAATATGCTTTGAAACTACTCTCGTCTTACTACAAAATCGTAAGTGTAGAGTACATTCATTACAAATGTCCGTCAATGTTGACAAAACGCCAAAACCAACTTTACGGTACGTGGATAAATTATGCCTTCACCAAATGGACCTGTTTAAAACTGATTCAATACGATAAAATCATCTATTTAGATGCCGACCATTTAGTGATTAAAAATATCGATCATATGTTTGATTTAAACGCGCCCGCTATATGTTTCACTGACGAAAGCTATGGGTACTACGATAAAATCGTTTACGGTCAAACTATATCGTCGCGCGCTATACAAAAGTTCATGCGCCAAAATAAAGTGCTATGCAAAGGTGGCACGGTTCTTTTCGAACCCAATGTTAAACTATTCGAGTTGATAAAGAAGTTGGTCAATAAATCGAATCCATGTTTGACAAGAAACTATTACCACAATGGTTTCGATGAACAAGTGTTATTACAAGCCCTCATAAAATTAAACATGCCCATAACGCAGCTATCCATTTTGTACGCATGGAACGCAGGCACATACCATCGTTTGAGCAAAAACCACGAACCTTTTGTTATAAATTACTATGGCGATCTTAAACCGTGGAATTTCGCCGATACAGATGTGATCAATTACATGGATGTGTTTATTTGGAGGTACTTTTCGAATCTAAATCTCTAGTATAAAAAGCGATTGGCCAGTTTAAATTTCATTCGCTCAACCGACAGCCATCGACGATGTCTCATTATTATTTGATTGAGAAGCTCGACGATGCCAGATACAACATCTTCACTTCAGAAGACGAACCTCGAAATCATCGAGGCACCATTATCTACGAAAAGGACCTAAAACGTTTCGCGCCCATTTATCTTCGATCTCAACTGAAGCACTTGGATAAATATAAAAATGAAAATGACGAGTACGTTTTGGAAAAAGAGCTTGTCGACAAACTAATGTATATGACCGAAATGTATTATAAAAGTATTGAAAGTAGGCTTTAATAAACGTTTTGAAATATATTTTATATTTTTTTTGCTAACCTTAACCCAACGAAACTTGAATATGAGTAAAATCTTACTCATATTCAAGTTTCGTTATACTACTAAAACGAAACTTGAATATGAGTAAGATTTTACCCCAATTCAGATTTCGATAATATATTTTTTAGTATTCGTTTGTAAATAACGAAACTTGGATTTGAGTAAAATCCTACTCATATTCAAGTTTCAATTATTGCACCATATTTATGGCATCATCCAATCGAACTTTATCATGATAAGATAATCATGTCTCGTGTACAAATTTACAATTTTTTTTAAACCATTCTATTGTTGGGTAGCGAGCACGTACGATGGATATGTGTGTAAAAGAAAACCGACTAAAAACTTTCGAATTATGGCCGGTGAAGTTTTTATCGCCAGAGTTGATGGCTGAAAACGGTTTTTATTATTTGGGACGCAGTGATGAAGTTCGTTGCGCTTTTTGCAAAGTAGAAATCATGCGTTGGGTGGAAAATGACGATCCCGCTTTGGACCATCAGAAGTGGGCACCTCAGTGCCCGTTTGTAAGAAAGCAGGTCGACGGTGACGGAAGTTCCGGCGGCCCGGACGAGTGTGTCGTGAGCTCCTCGCCTAGCATACCCGGCCCTGTACACCCCCGGTACGCCACCGAGCACGCCCGTCTGCAGACCTTTAAGGATTGGCCCATAAGCATGAAACAAAAACCCCACAAACTTGCCGAAGCGGGTTTCTATTACACCGGTTTGGGCGACAAAACTAAATGTTTTTTCTGCAATGGTGGCTTAAAAGATTGGGAGGACGATGACGATCCGTGGGAACAGCACGCTAAATGGTATAGCGATTGTCGTTACGTTATTTTGGTAAAGGGGCAAGATTTTATTCAACGTGTACATTCCGAAGCGGCGGTTGTAAAAAATTATGTTGAAACAACCGAAACTGTTGAAGAAATAATTGATGATTCAAAAGTGTGTAAAATTTGCTACAACGCCCCACTTAACGCCGCTTTCAATCCTTGTGGTCATGTCGTGGCGTGTATCAAATGTTCTGTTTCGGTTAATAAATGTCCTACATGTCGAATGCCTTTTGAAACTATTGTAAAACTATATTATTCATAATAAAGTTTGTTAATTATTGATGTTTGTTTGAATTTATATTGAATTATGTTTGGATAAAAGCTATATACTAGGTAGGATAAGTAATTTATTAAAATTAACGATCTAACTACAATATAATGTCTGTGTCTATTAAAAAAGTTCAAGACTACGGCGTGCCGGTGCTGGTCGATCCCGTTACGTGGACGGCCTGGGTCGGCGCGGACGAAGTCCTAAATGTTTTACGTTTGCCATCGTCGGTGTTACAATCGATTCCGTTGCGCCATAAAAAGTGCTGGTTAGATTTTAGAGGAGGTTTTAATCCAAACAATAATTGTAATGTTAGTTGCCGGTGGGACACTGGTAAACTTTTCATTGACCTTTACGGTTTAGGATTATTATGTGGTAGAGTAAATTCAAGTTTGTCTGATTATTTGATGACCCAATTTGTAGGCGAAATTTACAGAGATTATGCGCCTGATGTCTTACCGCAACCTCAACCTCCCTTACCGTTCCCCGTACCTCCTCAGCCACCGGTACCGCCGCCGGGCAATTTACCTTTAGAATTACTGGAGCGGCTTAATAGGCAAAGCGATTTAATAATTAACGCTTTAAGTCAATTGAGTATCAGTAACTCTAACCAACACTTGGAAATTACAAACCAACTTAACGCTATCCGATTACAGAACGTGACCATTACCGGTCAGCTAACAAGTATACTTGACATTCTTGAAAATCAATTAGGTAATGTAACTAGCGAATTAAACAGGTTGTTGACAGAGTTGGACGGACGTTTCGATAGTTTTGTAAACACGCTGAACAGCGCTCTGTCTGCATTACAAGATAGTGTTCGAAACGAGTTAACTACAATCAATTCCATACTTAGTAATTTGACTTCGACCGTGACCAATCTGAACTCAACGCTGACCAACTTGTTGCAAGCCATCTCCAACTTAAACCTTGGCGATCTTAGCAACGAAATCGAATCCATATCGACTACCGTAGATCAAATATTGAGCATTTTAACTCCCGAGATTCAATCGAAAAAAGCTTAAATAAATTACTTAAGACTTAGAATAGGATATAGGATATAGGATAAGTTTAATTAATTAATAAAAATAGTGTATAAAATTTTTTTTATTAAATATTATCATAGGTTTCAACAACTTTATAAATCTTCTCCACCAAATCATCACAGTATTCGTAGGGTTTCACATCAATAACATTCATGTAATCGTTTCCAATTGTTTCTGATACCATGTCGGCGGGAGTCGTGGTGATTCCGACCGCTAAACATTGGCCGTGTCTGCTCATAACGGCGTCGATTATATCCAAGTTCATGAGACATTTTTCGTATATTTCTCGGCCGTTATAATTTTTATTGTTAGGTATCGAAAGTACGTATGCAATCAGATTCATTGTAGTACTACATCATGAATATTTTAAAATCGGTTTATATACTACCATTATTTTTTTTTCCAAAATTTACGTAACGCACCTATTGTTATTAAATTATAAAACTAGTATCTTTTTTAACACTTGAAACGATTTTTGGTTTACTACAAACTTGCCCACTTTTACAGTCTGCCACTCCAATAATACCGCAACCTAACCGTCCACCCGAATTGCCCGTAGTTTTGCTAAGAGGATGATCGGTAAGGCCAAGATCGTCACGATTTGTGTGCACTACTAAACTGCGGCCCAAAATGCTGTAATTACCATAAAGAGACATGACATTATCGATTATGTCGATTTCGGTGAGCGAATTTGAGGTTTTCGCTTCAATGTTGCCTAAATCTCCCACGTGTCTTTCCTCTGAGTTGGGCGCGCCGTGATTTTGACCCGTTGGGTTAAAATGTTCGCCAGCCGACGTACACCCGTTACTTGTATCGCCATATTCGTGCACATGAAATCCGTGCAATCCTTTGGGCAAGTTCATAATGTATCCTTGAATTTTTAAAAAGTGCTTTGGTGATTCTTGAATGAAATACACCTCGCCAGTAACGTCACCATCGATTTTACAAATGGCTTTCATATCTGCAATCTTATCTAATATGTATAAATTGATGGACGAAAACGCTTTTGGCACATTCGTGTTCAAGCAAGCGACACAATGGGTCACAATATTGACAGTGTCATTAATCTTGGCGATATGTACTTTTGCGTCCAAGAAAAATACATATTAGTGGACGAGAAAAAATATTGTTTAGATTGCGCGGCCGATTTCATCATGGAAGATCACATAGTCTGCATCAATCATGGCCCCAAAAACGATATACAAGATTATTGTTGGAAACTTGAAAATCTATGTTGGAGTTGCGAGAAAAACGTGTATTCGGTTCATCGACTCAAAGATTGCGACAGCTGCACCGAAGTTGTGTTTCAACTTTACGAAAATGTTGTCAATGAAGGCGCCATTTCTGTAACTCTATAGTTTTAAGAATAAACATTATGAACGAATTTAGTGTTTTTATTTTCGCTGTACTAGTGTCTTTGTTCATAGTTTTCCTTTATTGTGTTAGCACAGTTTCTAAATTAATACAAACAAAAGAAGCCGAATCGTTTTCGCCGGCTTTAGACATAATTTTTGATCGTAACGGCATAGTCGATTGCAATCTAACCAAACTGCCCTGTGTAACTTCGAGACAATGTTTAGACAATTGTAGCTCTCACAATTTGGTGGGGTCTATTGTGTGCGATGAGGGATTTTGCACTAGTCGCGATTTAAACGTTTCGGGTCGCCCCGATAATTTCGAGTGCGACAGCTCGTTAGGATTGATTAAAGTTTTTACCGCCAGCGATTTTACCGTGACGAATTTGTGTGTAAGCACATACAGAGACATTATCGACGATTTTGGTAATAAACGGCCGTATGTATGTGATAATGGCAATCTAACTATCGATCTAACAAATAGGCAATTTAGCGCGGCCGATTGTGTCTGCGACACCGGATACACAAGAATGCTATTTAATCAAACGGCCTTAACTCGTTCTGTACCAGTATGTATTCCCGACTCAAACCTAAACATTTACAGCAAAATTTACGATCAAGTATGAAACAAAGTTTCAAGATGAATTACACCGTTGAAGAATACAAACATATTATTTGGGCGTACGTAATGAACGTTTATGTACAAAACACAAAACTCAGATCGATATGTTTGCGAAAACCCGACGTAAAATATGTAGAAATGGTTGTTAGATTGCTACCCAAAATGGACATGAAAAAATTGTTACCACGGCTGAAAAACATATACGCGCTGCACCGAGTCGACGAATTGTTCAAGGAAACGGACAAAGGAGTTTTAGAATTAATAAATAGTATTAATGAATGTTACAATGAATAAAATGAAGTATTGTTATTTAAGTCTTTTTTTTTACAATGAACGCAGAACATTTGCTCCTTTCCCAGTATGTAAACATACAAAATAGCATAATAGAACTGCAAAATAACTACCTGGCCAATAGCAAAAACCCCGCGGTTATTGCCGATGTAATTAAAAAGCTTACGAATATTAGAGAGGATAAGTTCCGTACAATAGAATCTAGATTGAATACAATCAATGCTAGCGAGCGAACTGCCACATTTTCACTCATAATTAAAAATGATAAATCGTATTTGGAAAATTTTTTAAATGTGGTCGATGCAATTTATTATAATACCAATCACGAAATATTTGTTGACGAAAGTTTAGTCGTTAGTCGTGGAGCGTTTTTAGATGACTTGATAAATTATGGCAACATACTCGGCAAGGAAGATTTGATATATTTTAAATATGAAGTCGAGTTGATAGAATTATTGAATAGACCTTTGAAAGAAATAGACTCTGAAATGCTCGAAATCGACCGCATAGATTGCGCATCCATTATTGTCAAGGCATTTTTTCTAAACAATATTCCAAATGTGAGTTTTGAGTTTTTAAAACAAAATATAGACAACGATATAATAAAAAAGAAACTTTCGGCGATACTTTCCTATATTGCGGGAGTATGTAAAAGAATTCGCGAATGTAAAAATAACGAAAAGGCTATTTTCACTACATTTAAAATATTTTACACAGAGCAGAATGAAATCAATGACAATTTGACCGTTTATGGTACGTTTGAAAGAAACAAAATTGATGTAAATATGATTAGTCCTTTGAAATTTAGAGGGGCAACATCACCTGAAAATTTATACGATTTCGTGTTAATGTATACAGAAGGTTTTGGTTCTGTCGAACCTTTCAATGACACTATTAATTTTGAATCCCTTATGTATTTGACGTATCCAGAATTGTTTGCTATGCCGTATTTGTTTAGAAACAGCACTGATAGCGAACATCGTTTTTACAATTTTCAAAATCTTGCAAAATCCAACACGATAGAAGTGGCGAGCAATGGTGGTATAATAATAGAGGAAAACGATCTGGTAAATGTCGACTCTGTCCTGTATGTGAACATCGCATTAATATTTTACAAGTTTAGGCCTGAATCTCCGCCCGGCACCAAAAGTTTTCTAGACCGACATTTGAATAAAATAAAAACATGTTTATATGAATACGCCAAAGTGGACGATACTATTACGGTCTATTTAAATCCTAACACTGCAAATTACCACAACGAAGAATATCAATTTTTAATCGAAATGTTGGCCGTGTTACAATTAGGTTTTTTCATTTCTTACAACGCTTTAACAGAGGAAATACGAAGTAACATTAACGACACATTAAACCTAATACCAGACCATTCTCCGCTAACACATATATACGATAAACTTGTCAATTATAACTTTAACATTAGTGGGGATTCTAACATGGAAAGTGAAACAGATTCTTTTTTATAACAGTTTAATAATATTTATTCGTAACAAAATTTGCCGGGCCTTTTTAACAATTACAAAAACTGGCCGTGCGCACAAAACATTGTTCACCCAAACACAATCGTCTATTTGCTCGAACTCGTACCGCTCAAAATCGTAGCGATTGTTGACATAAAAATAGTATTCACCATTTCGACGGCGGTAATCGATAGTTTCTTCTCGAACGTGCAGCTCTTGAAACATATTGTTGACAACGATCTTTACGTGGTCTTTCATAATAAGTGCGTAGTCCAAAATGGATCTGAACATAGATATCGACGACTTTACCAGACTGTTGATCGCTGACAAATGTAACGCATTAATTGCTGCGGAGCGGATGTTGCCCGAAAACATCCTGGCGATAGTCAAAAGCGCCCGCGATAATTATTTTGACGACCCGTCTCCAAAAAATTACGAAAACATCAAGAAACTTTTTAGTCAAACCAAATACGTAGACGACGCGGTTGATTACAAGGATTTTAACCGTAGAATCATGTTGATCGCTTTCAAATTCGCTTTGAACAAATCGAAAGAATATTTTAAAAACTACAAGTCGGTGTTGGAGGTAGCGCTCAAACGACTCGACGGTATAAATCCCGACTTGAAAAGTTCACCGCGCGCCATGTTACAACATTACAACGAGTGTTTAGAAAACTTGGACAATCCGCGTAACGACGAACATCACCTGGTCACTTTTGCCAAAGAAATTGCTACAAAAATTTTTATTGAGACCATCGATTTGTACAGTTACAATAATAAAAGTCCATTCGAAAACGGCAACGCAACATTGACAGGTTCCGATTCTGAGTTGCTGGCCGTAGCCGCGGCGAGCCGAAAACGGAAAAAAAATAGCATTTTGACCTACAAAATTGTCGATCCCATTTTCGTTTTGTAGAGTTTAGTTTAAAAATATTTTTTTGCGCTCCAAAATTAACGAACGACACATAACACATTGATCGACTTTGGATGCGCAATTTTCGCAACATACTAAATGACGACACGGTTCAAACAGAATACTTTTTTTGCCGACCATGCAAACTACGCACACGAAATCATATTTGGGTGTTTCGGTTGGAACAAAATCGGGCCGCGTTTCTTCGTCAAGTTTTGAGAGAACATATTTACACTTGGGCGAATATTTAATGTGTTCTATCAAATAATCACTTTCAATTTCGGTCCAGTTAAAAATTCCATATTGCAATCTTTGCATTTAATTTTGTTTTGGTTACAGTAGACGAACCCAGCATTTTCGATTTTATCCATTACGTGTAATCATTGTAAATAATTTAGTATAAAAGAGGAGACAGGTCCTTATAATAAATAAAACACATTTTTGTTATAACTGTTTTTTATTAAAACATATCATTTTCATACTTGAACTTGATTCCGCATGCGTGAGCAAACACCGTGTCTTTGTTTGTCGTACCATTATGATTGTCTCCGCAAAAAAAACTGTTTTTACAATCGATCGAGGGATTCATTTGCAAAAGTTTTTCAAAGAATCCCGTCGATGGAAGCTTGTATTGAATAGTATGAGACATCATAACAAACATGGGCACGGCAATTTGCTTACACACATCCTCAATTTCACGATCCTTACTAAAATTAGACACGAGAACAATAGTAAAGTCTTGGTTAATTAAATTCGTAAATTTGTAGACTATGTTGTCGTACTTAAACTGCCAATTATTATTAACTTTAAGCATTATAGTTTCGTTTAAATCAAAAATGGCTACTTTATTTTTCAGTATAGGCAAGCGGTGGTGATAAATACTCAAAGAATTAGTAATTTCCCACCGAAAGATTATTTTCAAAATGTAGTTGATTATTTCTTCCTGTTGTTCATCTAGAGAAACCTTAGTGTCCACGATTATTTCGTCGTAATTCATCACTCTGCGCCAGCAATCAAACTTTTCGTTCTGCTTGGTAACATAATCCGCTTCGTGTATGTCCAGACCATTGTTGCGTTTCTTCATCATTTTAACAATCTCGTTTTCACTTCCTTGTTTTACGCGTAAAATTAAAGACTTCCAACCGTCACAAAGCCCCATATTCTTGCAAATTTCAAAGGCTTGTTCCGATTCTTGTTCGGGCATGTCTTGGAACACTGCCGAATACACCAACGCCTCCATGGGTTGACGATCGAAAATGTGAAAACGAGAATGGTCACGCTTGTATGTTTCAGCGCTCTTGCTGCGATACGCCGCGAAAAGCATACCGCCCACATTGCTGGGGTAATTATACTTGTCGTGTAATTCTTTGTAATCCTCTAAGTGGACACGAGCGTGAGGGATTCTATCTTCCAACCTTTTAAGGATGGTAGTTTTTGTGGTGCAAGCTACACCTCCCAAAGCGAATAAGTACGACATGATGTGACGGTAATAATGTTTGAAAAGAAATCCGTTATTCTTTATATAGTGTATTAATTATCTATAGTAGATTAATTATATATAGTAGATTAATTATCTATAGTGTCGTCTGAAAGTCAAAGTCATCAAACGAAAATAAGATATGAGTAAATTTTTACCCCAATTCAAAGTTCATATACTATTAAAAACGAAACTCGAATATGAGTAAGATTTTACTCAAATTCGAGTTTCGACAAACGAAATTTATATATGAGTAAAATCCTACTCATATTCCAGTTTCGTTGTTGATATCCACCAGAGCGAACGTGCGTTATTTTATATTATAATGGATTATAGTGCAAACTTGATTATAGACGGCATATATCTAGGGGAATATGCTTTAGAACTAAACTATATGCGCAAATTTATCCATGACAACGACATCACCAGCGTCCTGTCCGTCTTTGATAGCCCGCCCATTGAAAACATTGCACCAAATTATCTCTACGTTTATTGCGAAGACGATGAAAACGACGAAAACATGGAATCGCGTTTGGACGACATGTACGAATTTTTAGAGAAAGCCGTTCAGAGGCGCGAAAAAATACTGGTTCACTGCCACGCTGGAATATCAAGATCGGCCACCGTGGTCATTTACTACATTATGAAGACCTATCGGAAAAGTTTTAAAGACGCTTTCAACATGGTCAATGAGAAACGGTCCATATGGCCCAATGATCATTTTAAACGCATCTTGAAGAAAAAAATTAACAAATAAACAATTATTATTTGTAACAAATGTTTTTTATTTAATGAAATGATATTAGCGTATCAATTTTTGTATTCATTTCGTCAATTTTTTTACTATTATTCGTAACTAGTTGAATCGTTTCCATTGTGGTGTTGTGGATTTGGTTTAGGAAAAACATGGTCTCGTTGCGCATGTGTTTTATTTCTCGAGTCATGGTATAGAACTTTGTCTCGATCATTTCGTATGTGTCAGAGAGCAATTCTTTGATGGCGTTTATTTCCTCCATGAGCGAACCCGCATGCCACAAGTATGCCAACAACAAAACCAACGCCACACACGAATAGTTCATTTTGATTTTTGAATGGGTCCTCTTATTTTTTACGAACAATCATGTCTTTTAGCGATTTAAAGTTATCAAGCATAGTATCATTGCTGTACGCTAGTTTGTTTGTTAAATCGATTTGTAACGATTTTATATTTTCCAAAGCCAGATTAAAATTACTTAACAGAGTGTTGTGCTCGTTTCTTAGCAAATCACGTAGAGAGTCGGTCCAACTGCTCGAAGAATCATACACGGTTGGTTGATATATCGAATCTGGCGAGTCCGATTTGGGCGCGCCGTTGGTTAGGCAATCTTTAATGTGACAAATTTGACTCTTGATATCCGACAGAGGGTCCACCACTTGACTCTGCACACCCATCATCAAATCGCAAACGATTCTTTTGATTAGGCTCAACTGTTGAGTGTTAATTGATGAAGCTAAATTTAACGTGGCCAAATATTTACACAGCGTGAAAACATGAACGTAGTTTTTGTTATTTCTAGTGAGACGCTGCGAAGGAATTACATTTGCCCAAAGCACGGCTTTGTTAAATCCCCTAATAGTGACAATGTGTGCGAGTAAATCTGCCGCAGCGCCTAATTCTATGTAACCGTCCCTGTCATCTTCGCCGTTTTCAATTATTACCACTTCGATACTTTCATCGTTGTAATGAAACCGAGTCGCCTCAGAATTATGAACGGGTGTATTTTTATACATGTCGGTAATGTCTTATATTTGTACCGGACCATAAGCTAAGTTAATTTGATTTTTTGAATTAATTATTTCATCATGAATTACAACCATCGCGACGTATATCGTCGCCGCTACGATAGACTTAGCGAAAAATACAATGCCGTGCTACATCAGCACGAAAACTTAAAATCCGACTTGCGCCATTTAAAAATGCAGATTCATGAGGTTTGTCGCAATTCTGTTGGCGCGGATAACGTTATATGTGAGCGTATACTAAACAATACACCCTTGTTAGATAATACTATCCACCCTCGAAACGACTATACAACAGCATTAGTCAAGGACAAGACGCCTCAACGTAAGCACATGTACAATGACGGAAAGCCGTTGGTTAGTGTGGAACCCTTCGATTAAAGCATCACAAATTGACCACGATGCCATGTATCTAATTTCCATGGAAGACTTTGATGTAGAAGTTTCGCCGTACACCGTGTTTGAGCCTTGCGGTTCAATGAAAGTGTTCATCACGGGTTGTAAACTTTATGATATGATTAAAGTTTCTTTAGAGAACGAGCACAAAGTCATGTTGAAACGGTCATCTTCCAACGGCGAAGACAGACTTAACAACAAGTTTATGAAAAAAAGTCATCGAAACGTTTGTTTCAATCGGGTGACTGATAGAGCCAGCATTATAGGATTATTGAAAATATCATTGAAAATGCCCGAATGTATGGAGAATATTTTTGTGTCTTTGATAGAGTCGCCGAGAGGAGGCAAGCACTACACACGTTTTGTTTTCAATTGCTATGTTTGTAACCTGTTAACTTGCACAAAGTGTAACAAGCGTTGTTTGGCTTCCGCTTTGTGTACTTTATATCATAATGACGATAAATGTGTTAACGAAGTGGAAAGCGCGCTTTTTAAAAAAGAAACCATCTACAAGCCACCCAACTGTGACAATATGAAAAGGAAAAAGTTATGTTCGCCTTCTAAGCAGTGTTATGTTAAGAACCCTTTATGTATGTTTTAATAAAAATATAAGCACATCTTTTTTTGTTTTATTTAAGAGTATAAATAAAAATGTCTTTTGACGAACGAATTATTAGCCTGTGTTCGAAGGAAAAAGATCTGCGATCACAATATGAATCCAAAGTGAATTCGTTTTTTAAAAATAAAGGTATGAAAAAATCTGTAGACATATTGCAAAACGAATTACACCAGTTGGATGCCCTCATATTTGGTTACGAAGAACAGATTCATTTCCTGAGCACGAACAATAATGTCGCTCGCCAAGAAATGGTGGACAACGTTAACGATTTGGATCATTTGGGTATAGACAAAAACTTCATTGAAAGAATGATCGTGGACAAATTAGATGATTCTTTATTTGAAATATACAATACAGAAATTCTCAACGAAAATATAATTAAAACCTTTAGAAAACATAGTAATAAATTTGTTAAAGTTTTGTGTCAATTTGACGACAAACGAAAAGCTTACAGTAAAAAAGAGTTTAATAGAGAAAAAAACAAGAAAAATATTGATGAAAACAACAATTTATTAGTAGAATTAATTTTATTAAAATCAAACTTGGTGTTTCATTTATGTACAATGGAAAAAATTCTCGTGAACAGCGCTAACAAAAACATAATCAAAAAAATTGCATAATATTCATGATTTTTGATTTCATGTTTTCAATCTCGTTAATGTCTCCGTATTCTTGTATAGTTGTTATGTTTCCATGTGTTCTTTGCAAAATATATCATAGTTTTTAAAGTTGACAAATCCATTGTCATCCAACGAAAAACAATCTTCGCACACGGCCAGGGACACGTTGGCACCGTGCACAACAAACAAGGGACAAAAGTGATTCTTCAAAATCGTGTCTGCTAAAATCGCATTGTGACCCGGCAGCTGCGTCTTGATTTCCAGTATATATGCGGCGATCTGCATGATTATTGTGACTGAAGGAAAACTTCGCTTTCACTTTCGTTATATATTTTTTTTGAAATCAAATAAATCATTAAGAATGTATCTACTGTTATTATTGTTAACCCTGCTATTTTTGTCTCTCCTCTATAAACCGCTGTACGATGCCTACACTAAAATTAAAGAAACCCAAACACACTACAATGCTACGGTCGATGATCGCATCGATTACATGGAGACCGTTTTAAAACATCGTCGTTTCGTGCCTCTGCATGTTTTACCAAACGTAATTTTCAACACTAATTTGGGTACGCTTAACGAAGGCGATGTCAAATGTTTATCGGTCCCAATGTACGTTGGCATTTACGATACTCCCAATTTTGATTGTACAGCTTTATGTGACAATCCGTCGGCAGTTTATTTTTACGTAAACGAATATGACAAATTCGTTATCAACGGAGATTTATTACCAAGAGGCGGTTATTGTACCACTAGTAGCGTGCCTCGCAATTGTAACAGGGAAACTAGCGTAATTTTGCACAGTTTAAATCAATGGTCTTGTATAGCCGAAGATCCTCGCTACTTTGCCGGTCCCCAAAATATGACCCAAGTCGCCGGTCGCCAGCATGCGGACAAAATATTACCGGGACAAGTCGATAAAAATGTGCTAACAGATCGGCTTTTAGGAACCGAAGTTGACGTTTCCAAAAACACTTTTAGGTCGCATTGGGACGAGCTATTAGACGACGGCACGAGGCGTTTCGAAATGCGCTGCAACGCCAAAGACAATCATAACAATCAAATGTTTCTCAACCCGTTCAATCTCATCGAGTGTCTGCCCAACGTGTGCACAAATGTCAATAACGTTCACCCCAGTGTCAGACCCATATTCGAAACTGGCGAATGCGATTGTGGAGATTCTAACATCACCCGTCTAACACACATAATTCCCGGTGACCGCACGTCCATGTGCGCATCCGTAGTCGATGGTTTCAATCGAGATTCGATGTCGCATCAATTTAGAGTTGAATGCGTGAACATGGACATGCTGTTATCGGAATTCAGCAGTACTAAACCCCTTTGTCCCCCTGATACTTTTACCCAAAACACAGACAACGCATTTTTGTTCGAATTGCCAGGTTCTTTTCCTTTTTCGGGCAACGGTATCGACGAGCCCACTTATAGAGTGTACATGGAAACTAGGAACAGGATAAATTATAACACAACAAGAAGTATACCAAATTAATTTATTTTTTTAATAGTAAAGTTTAAATGAAACAATCGATTTAATTCTTGCAAGTAAATTTTCCAACACTTTGAAAAATATCCTTGTTCTTTAACATACGGGAAGTTTATCTGGTGGGTGATGTCCGTTATGCTGTTTCGAAATCGGCCGGCGAAATATAATTCAATAACGAGGCGTGCCCCAAGCCGCGGGTTAGTTTTGTAAAACAGCGTTTTTACGTTCCTGATGTTGATCATGTTTTTGTCTCGCTTTATGTTTGCATATTTTTGTAATTGCTTTTCTGTGGTCGGCATGTTGTACGACCGCAACAGGTCCTTCAAAATCTCCAATCGGTTTTGGATTGAAGGCACGCGATTAAATACATCAAACAACAAGCATTCCAGTTGATCGGCGATAGTGAGATTGATATTTACCACACAAACATATGTGTCGAACTTTTCGACCAAATTGTACAAATTTATTTTTTCATAACTATTCTTTATAACTAAATCCAGAAAGTAAACATCGAACGGAAAGTAAACGTACGTGTTAACCGAATTGTCAGATTTGTAGTTCCAAATTGGAGATGCGCTTTTCATGTGTACATTAAACGAAAACCGTACCAGCAAAAACTCGTCGTTCACCCACGAAGAAGTGGTTTTGAACGGAGCATTGCGATTGATTTCAAAGTAAACATCGTTCACAGAATCGATTTTAATCGCTTCGTTTACGTACGATTTGAAGAACACGAGTCCATTATTAAATTCTTGATCGAACTTGCTACCGTCAATTTGGTTCAGCATAAATTTCATGTCAATCATTTTTAATGTTTGTTCGACTTTTATGATGTCGTCTCGAAATTCATTTGAAATGGCATTTTCGAATAAGGCTATTGGTAACGTTTCTAATGCGTTTTTTCCCTCATTGATATTGTCCAAGTAAATTTCAATGTTTAGATTGTTCGTCAGCTTGTAGGGTTTGATGGTTCGGTAATAGTCCGTGTAGCTATCGATAGCAAACGTGCCTTTGATTATGGAGAATTGATCTTCGAAAACGTTTCTAAAAGTTTTGTATAGTTTACAAAAAAAGTTTTTGTCGAACCGGTCTAAAACATCGCTGCAAAGTTGATCATTTATATTTTTTTTTGAAATATAAGGTATGGTTTTTAAATAAAACAATTGTTGACGCAGGGATTCCATAATGAACAAGGCGGCGTTAACTTCGCGACGTCTTAATAATGCACAAAATATTTTGGCCACTATTGTGGATATCAATTCTCTTCTTAACGTAACGGACACCAGAAACAAAAGATTGTTTTACAATTTGTGCTCGCGCTATATAAATCAAACACTATTTCAATTTACGAAAGGAAATATGGCGCTAGCCACCGTTCAGACGGTTTTGGATAGCGTCATATCTGCAGAACGTATGTTTTTCTCAAAAAGCAATGTGCTAAATATCATTGTAAACTTTCTGACCGCGCACAGCGACGGTGATAACCTACAGTGTATGATTAATTTAAAACTCCTAGACTATTTTTTACACAAGTATTATTAAATAAAAATACACATGAAAAAGTTTGATTGTTATTTATTAAAATCTTACACATGAAATTATACAAGGTAAAATACACATGAAAAGTTTGATTATACAATTGATATTTATAAAAATCTTAATATATACATGATTGTTATTTATTAAAAATCTTCCATAATCTCAAATTTGTCATCTCCGGCCCCGAACCGCTTGTACTCGCCAACTCGCCTTTCGAAAAAATTCGTTTTGCCTTCCAACGAAATGTTGTTCATGAAGTCAAATGGATTGGTCGCGTGAAAGTACTTTTCTTGATTTAGCGCCACCAAAAGACGATCCGCAACAAACTCAATATATTGACACATTGATTTTGAGTTCATGCCCAGCATATCGCAAGGAATGGCAGTAGTGAAAAATTCCTTTTCAATTTCCACAGCTTCTTTAAACATACTCAATATTTCCGACGAAGTCAGTTTGTCATTCTCAGCAATGTAATTGTTATAGTATAAACAAGCAAAGTCGGTATGCAAACCTTCGTCTCTGCTGATTAATTCATTGCTAAACGTCAGACCCGGCATGACTCCTTTGGTTTTTAAAAAAAATATAGCCGCGAAACTGCCAGAGAAAAATACGCCCTCCACTATAGCAAACGCGACCATACGTCGAGCCAGTCTGCTTCCTTGCGAAACTTTTGTGGGAGTTTTCTTGAAATAATTATACACTTGCTGTACGGGATATACGATGAATCTATCCCACAGAGTTTGTTTCTCTTTGTTATCGTCTTCAATCCATTTCATGGCCCAGCGCGCTTTGTTTCTCACACAATCGACATCATCGAAAGCATTGAGTAACATGTCACGTTCGGTTTCGTTTGAAATGTACTCAAAAATCAGCAAATTGTACATTTCTGTGTGTACATTCTCAATGAGTATTTGCTGGTCGTAAAAGTAGCGGGCTTCCAGCTCTGACACAGCGTCTCGCACATATTCTATGAGGTTGATGTTGACGATGCTGTCGGCGGCAGCAAAGAAGGCCAGAACATGTTTCAAAAAGTGCCTTTCGTTGTCATTGAGCTTTGCCATGTAGTCGTCGTAATCTTTTGACAGATCGACTTCCTCGACTTTCCACATACAATCGAGTGCCTTTTTGTAAGCCTCCCATAGTTTGAAATGTTGAATGGGCAAAAGTACCTTGCGTGTGTTGGACATGTTGAAGAATAATAATAAATTGAGGCTGCGACGCCTTTTATACCAATCAGGAGTAGTACCTCTCACCTACGTCATCGCCTACTCTCGCCTTTGTGCCGGATAGCGTCCACAGTAGTATTCGTTGATATTTTGATATTACAATATTTATAAAACGGGTCACATACGGATTTTTTTTCAGTTAAAATGGAGACTCTGTCGCCCGAGCAATTAATTGTGTACAATAAATTTATGTACAGTAATTATGTAACAAATTTGACATGTCCAACTTCAAGACTAGACACTGAAACCATTTTAAAAGTGGAAAAACTGACGAGGGGACAATCCGATAATGCGCTATGGGTTTTGCTGCGAATGGACAGACATACGGCTTCGGGCTCGCCTTTGTCCTACAGCACATCGCCGACGTTGGCCATGACGTTTGGCCACAATCAAGAGCGTTTGGTCAAAACGAAACACGCTCTGTTGGATTTGCTCGAAAACACCATTGAAACCGCGCTAGGATGCAGCGTGGTGGAGCGCGTTCTCGATTGTGGCATGTTTTTTTCATCAATGGGTTTGTTTTCGGCGTCGCCCGACGCTTACTTCGCCACCAACAAGGGCCAGTACATTCCGATTGAAATAAAGTGTCCTTACACGTATAAAGATGTGACTATTGACGAAATCAGAAACGAAATGAAAACGCGCAAAAGTCGCTATCGTGTTAAGCACACTGCGTTTTCGTTAAACAAAACCGGACCGCCGATTTTCAGTGTAGAAAAAACGGATCCTCATTACAGACAAATGCAGAGACAAATGTATGTTATGCGTGCCCCTATTTGCATATACGTAGTAAGTTTCAAGGACCATTTCGTGGCAAGCACAGTTGAACGTGACGATGAATTCTATTTGTCAGAGTACAAAAAAGAAAAGAACATTTTTGATATGTTTGTCCGGTCAAATGGGCTTGCGAAACGCATGAAAAACCAACGAAATCGGATTGCTACTTTTCAAAATACTAATAAATTTAGAAAAGAAGATGTACTGAAGTTGACACGAAGAGGTTTGTACTTGAAAAACGGCGAAATTATTTGCGCAATTTGCGCGACAAAGTCCGACAGCGACATTGATATATCAACGGTCCTAGATTTACACGAGCAGTGCATGGATCATAAAGACAACGAGAACATTATTGAATGTAAACATCAAAAATTTTTCAATCATTCCACCAGGATGAAGTCACTGATTGCGGCGAACGTGGACTCTTCACATGCCAAATGGGGTTTATTTCACGAAGACGGTTTGTTTAAAACCTTTTGTTGTGACATGATCGTTACAGATTTTGTGCCAAACCACGCTACTGATTGTGATTTTGCTCAGATTAGATTATTTAGATAAAGTTAATCTTTTTATTGATAAGTTTACTATATAAATCGACTAATGTCCCATCGTTTCATCATTCTTATTGGTCTCCTTTAAAGGAATTAATAACATTTGGAATCTTTGAATTAATAACATTTGGTAAGTTTCTCTCTCTCTATAATTATATAATTAATTTAGAAGATGTAATTGTTAGTATGTTTTATTCATTTTCTTTTTATTGTTTTCAGGTAACATGTCTCTTCCTCTCAAAAATCCCTGCAGCTTATATTCTCTTTCTATTAAAGAAATAAAGAAGCGAATATTAGATGAAAATAGTTTTAAGGATTTGTCACTTCCTAAAGTTATTATTTCCGATTTGGAAACTTTGTATGCTGAGATGCCTCCAGGATGGTATTATTGTCCGAGAAGATTAATATGTGTTGTCGATAAAGATCTTTTTGATTTTAATATTCACAAAGAAATTTGTGAGTATTACAAACAAAAAAATCTTGTTGATTTCTATGAATGTATAAATACGTATGTTTACTGGCGGTGTTCTGATGAAATTCCTCCAGAAAACGATCTTCATTTGCAAATTGATTTGCTTCAAAATTTCATAATGAGATTCTTTCGTTTAGGTGATGGAATGTTTATAAAACCTTGGATGGGAGGCGCACTTGAATTGTCAAATAATCGTAATACTAAACTCATTAAATATGTAGAAGTAGATGATGATGATGATTATAAAGAAACGCCTCCTTCTTGGTTGAAGCTTGTTAAATTTAACGATGTAGATCAAAAAATGCTGTTAAAATAAACACAAATGAAACATGTAATAATTATTAAATAAAATTTATTATATTCAAATGAGTGTATTTATTTAATTAAGTATTTTTTAATAGAAAGGTATGAAATTTTTAATCGCGCTTTTAGCCGTTGGCTTGGCAAAGGCGGCACCGTCGGGCGGTTTACCGTTGTTGGCAGAGACAGAAACTCTCATTCAAATATTCGTCAACCACCAATATTTAACTGGGTCAAAGAATGGCACAGTGTCAGGTGGTCAGGATAAAAGTTTGAAAGGCACATATTGGCGAAGAAGTAACGAAAACGGAAAACAGATTTTGCTTAGAAACGCCGCATACTGTTATTATTTGTGTATAAACGAGTGCGGTTATTATTATACAGCGAAAGAGCCCAATTCAGAATGTTTATTGGGAGAATCTTTCACAGACTTTGCTTATACACAGATGTTCAAAGATCACGGTAAAAAGAAGGCTTATGTGGCTTTGAGCCAGTCGGGAAAGATTAGAAGACTAATGAGTAAAAAACTATCAAACAGCAAACTGTTAAACGCGTCAAAAATGACCATTATTAGAGATACTGTCGAATACACGTTTGAGTGTAACAAAATTCTCAAAACAAAATTGAAATTTGTGCCGGTAAAAACTTGTGTGAATCCACCCAAAAGGATGAACCACAAGAACGAAGCCGCCGACGATGATTATGATGATGAATCTGTGGAGACTGTTCAAAATTACTACCACTTTGGTGAAGACGAGATCCATTTTAATCTGTTGACAACAGATCCCATAACCACAACAACGACCGAATCGTCAAAAAATTCATCTTTAGATCGTGTAATCGACAAGCTAATAAATATTGATGTGGTGGCGGATCTACCGTCCGAACATTTTGTTATAAACAATCTAGTTGTAAATCAAATGTGTAAAATTTAACTACTTTTTCAATAAAACAAATTTTTTTAAAGTCTGTTTATTTGTTTCAATTTTAGCATGTAACTCTATAATTGAATCCAAACATTGATCCATCAATTGTTGGTTCTTTACTTCACCCCTCTCTATGTAGTCTAACGCTTTTTCGTAACATATCAGCGATCTTTTATAATATCCAATCTTTTCAAACAAACGTGCTAACGTGAGACTTTCATTTAGGTTTTCGATATCGTTTTCCATGTTAATTTACAGAGTAATTTGAATAAGTATTCAACACTTCGGCTAGTCCCGTTGGATTGTTGGTGTTGACTCTATTTCCTTGAACCAAAGCCACAACGCTATAGTCGCGTATATTGGGTAAAATTGCAAACAAAACGTTGATTCGTAACGCCACGGGATATATGTTATTTCGATATCGGGTCGCTGGACTCCATATACGCAACAAAGTTCCGTTCCTACTTAAAAAGCACCACGAATTAGTAAAAGTTTCCGCGGTCCAAACTTGCGCATTGCCATGGCGTCTTATACAATCATTATGGTTTGCCGTTATACATCTGCCCTCCTCGCCGGGATATAAACATGGCGATGAAGTTCTCGCCGACAATTGAATGTACCTATCAAATAAACTTTGATGGCTAATGTCAGTAACGTTTTGGTCAAGCAAATGTTCGTTTCGGGGCGTGTAGGCCGTGGAAAACTTTAGTATCATAAAACCTGTATGGCTAATATCGGGATGGTTGGTCAAGTACGGGTAGAGAATTGAGGCGTAACGTTCGTGGCTAAGTTGATTCACTCTAACCATTGCTACAACATCATCGTCTGACCGCGTCGTGTCCGATTGTGCCCAGAAAAACTTGTAGTCTAATTTTCTTAAAAACAATAACGATTGGTTAAACGGACGAATACATGCGTTGGAAACATTGGCGCTAGATTGACCAATCATTGTTTCATTAGTGGGACTGTAAACAGCAAACAAAGCGTCAACCACGGGACAACTACAATATTTGTATTCAATGTTGGAGTTTTCATTTTTGTAATACATTAGTTTTCCTAAAGTGCGCTGACCGCTAATGGGATCTATCGAGCAGGGATCCGCCACGCAAATATCATTTAGTCTGAGTTCTTGACGATAGCGTTCGTGAAGCGCTGGATGATCCAATCTAACGAAACCCGCTTCGCAAGGTGCGCGATGAAAAAATCGTTGATCGTAAATCACGTCTCGCACCGTCAACGGTCGGCAAAACGGCGTTTCTGTTTCTGAATTGAAATCAGACACGTAACCTTCGTTACACTGACAACGTAGCGGGCGTTCGTGTAAATCTACTATTGAGCCGTTGGGTTGACAGCCGACACTGACATTGCAATCTTCGTACATATTTAGCTGTGTAACCAAACCTGGTGTAATGCATGAACATAACAATGAAAATCCCGTCGAACTTTCGGCCAACAACCAAATTCCTGTGTTGGGATTGCACGAACGTGCTCTGTTTCGATCGAGAGCCATGCAATACGATTCGCCCGCCTCGATGACTTTTTCGATTTCGTTGCCGTCCGTGTCGTGCATCGTAATTACTGTACGATCGTCAAATTGCTGGCAATTTGCCAAACCCTCTCGGCAAATATCGCAATCCATATGTGTGTTGCACGGTGTAAGAGTTTTATGACATTCGTGCGAATTGCCTTCGATAATAATTTCAGAGGGAGGTTCAATTAAAGGAGTTTCAGAATTGTCAAAACGAAGTAACACGGTTTGAGCGGGTTCATAATCAGTTTTTAATAATATTATCAAAGTGGCTAGGATGATTACAATAATAATGAAACATACGATTATTACAATCATTGTAAAACTTACATTTTATTTTTATAAAAGTTAGATTCTTCCTTTTCGTTGTCGTCGTCAAATTTGTTTACAGGCTTACCGTTTTTATTTAATAATTTCATCATAAAACTAGGTTGGTTATCCTCAACGTAATGAAGTTGATTGTTAACCCGAATTACCGATGGCTTGTCCGATTTGCAACATTCAAACAATTTGCTGATCGTCCCCGCACAACAGCAGTTGTATATTTTGAAAATTAGCAAGAAAGCAAACGCTAAAATTATACATACAATAATCGTTTCGGCTGTTCTACATTTGAGACCAAACCAGCTGGCGAACCAGCACATCCAACTTTCCGCCGTGGCTTGATCCGTTTCTTCAACCATGGTGTTATTGTTCATACGATGTCTCAAATCTATCAGGCGTTCGGTCATGCCTTTCAAATTGGTATGGTCGAGATCGCTGTTAGAGTTTATCGCCAACACATTTAGTTTGTCGATATCGTTTATAGCCGCCGTTAAATTAAAGGTCGAACTAATGGGCACTGTTATCATCGTCTTTAGAGCTACTGTGTTTTTAATTTTTCTCAAACTCAAAATCACCTTTTTGGTATTCATTACACAATTCTTTGTACCATCTCCTCGAATAATACCCGTGCCCGCCTTGAGGTGATACTCAAGCACGGTGCATCCAAACAAAAGATCGGTGTCCTTTTCGAGAACATACAACCAGTTGTTGTAGTCTGAAATGGGGTAAAAAATTTCACTATCGAATTTGCCCACACGAACATCGCAATCCTTTTCAAAATCAATATTTTCAACATCATTATTGAGTAGTATTTTTATGTCGCACAATTTGGCTTGGTTAGATTCGTATATGATTTGCGGTTTGGAGCAAAACATTCTGTCGTGTAAAGTTTGACAATACGGACTATCATCTAAACGCACATAGTTGCGGCGATCTTTTGACAATCCAATGTATTTGCTGTTGGGCAAAACTATGGCACATTTGTGTTCGCCATTTTTGGCCCGACACATTGGTATAGGAATGACTTGAAACAGATTATATTCCACGTTGTTAATGAGGGGAATTTCGACAATAAAAATAATTTTTCTCATGTTAGTAATGAAAACGTGGCTTTTAACAAAGTTGTCTATCAAAATGTTCATACCGTTCATGTCAGATGAAACGGGCCACGCTAAGTTTGCGGGCAAATGTATATTTATAATTTCGTTAAGCAATCGTTTGGGCGTTAGAACTAAAGAATTTAAATGGTTTTGTTTTGCATCGTCCACCGACTTTTCCAAGTTTTCATACAAAAATTCAATTTGATTTAGCTGCTCTTGAATCAAATCGAACTTGGCCACGATATAACTGCAATATTCTGATCGTTTCTCATCGATGCATTTTTTATGTTCTTCGAACGAAGCCATTTTTATTAATTCGTCTGTTAGCGATTTTACTTGTTCGTTAAGGGCGTTATTGTTTTTGGCAAGTTCATTGAGTCGCTCAGCGTCATTACTGTCCATAACTCCGAAAAGATACTTGTCAACATTGCCCACAAAATTGAACAGTCCTCTTTTTTTACGAGTGTGTAGACTCAAATCTTTGTGATCTTCAAAACTGCCAGCTTTTTGAATTTTCGAATCCAGAGTATTATGATTGTTAACCAAATCTTTGATGCGTTTCATAATGATATTTTTTATGCCCATACTAACTAGATTGGATTGAACGCAACTACTAGACGCGTTTGTGGCGGTTTCGATTTCGCTCAACAACTTCAACGACTCTTCGTGTAGAGCGTTGAGTTCTTGAAAAACTTTGCTGTGATCCATTTCGATTACAAAATTCCATATGTTTTCTATGAATTGCATTCTGTTAGTTGGTTGGTAATAAAGTCCCGAAGTGTTAGGTAGTCGCTCTACGGTTATCAAGTTTTCAACACGAATTTCGCTCGTAACTACTAATCCTGCCATTGAGGCCGTTAATAAAACACACAAAAGTGTCGTGGTCATTTTGAAAAATATAATAGCTCGTTGATACCCTTATTAAACTTTTATTTTTGATAATTATTCACAACTCGTCAATATGCGAAACGTGTCGAATCTATTGTTCTCGTTTTTAAATATTATCGTTTTTAAAAAAATTTGTGCTCATAATAATGAAATTAGTAAAATCGATTCGTTAGTCGAATCATGCATCGATGTCAAGTTTAGTTCTGGTGAGTGTGAAAATAAATCCCTAATGGAAAAGTTCGATAAACAAACGTTTGACTTTGGCACCGCAAACGGCGTGAAACACTATACTTCGTGGCTATGTTTAATGAATAACATGGAATATTTTTCGCCGTACGATAAACCTCGATTGTTCAAAATTATGGCGCGCCACAATAACGCCATTGCAAAAATCGACGGTAAAAACATAACTAATGAAATGCGCCGAGTAAGTTCCGAAATGTTTCGATGGACATCCGACACGCTGACCAGATACCACGATCTGCAAATTATCTATGATACTTTCGATTCGTTTTATAATATGTTTAACACGTTCGTTTTATGGACGGGGCCGGGCAGTTTGTACCACTTTAACACGATTCTCTACGCGTACAAAAACGTGAGACATGCGAAATGTGCCAGGAAAATTGACGAAGCTGCCAAAGCGGCGGCAAAAATTGCCCTCTATTATCCCCTGTCCACTATGAACAAAACTGAAGTTCTCCAACAATTTTTCATCAATTACGTTTCAAAACTAAAAATGTCTGATAGAAAAATGTTTGGGGGTTTGTACAGCTACGTAAGTCAGACGAACGTTTTGCCGTTTTTGTTGACCATACAAGTGGGCTCGTACACTTTTAATTTGCATCATTACGAAACTGACAAGTCCAGAATTGATGCTATAAAAAATGAAACAAGATTTGTGCACGAGAATTTCAAGCGTTTCTACAAAAATTTAAATGTAAATTACGTGTACTACCAAATTGTGATTAACGGTTTCATTCATCCGACCAAACGAGCCTACACCCATTTTGGTTCGATGTGGGACATTAGCACGGACAACGGTGGATACACGCACATGACACAAAAATTGCAAATAGAATTTCATGTGTATTACAAAAACAAAGATGATTCTTTGCCGTGGAATTACGGCCACGAAATGCACCACAGCATGTTATACGCCGTAGATGCGGTGAACATAATGCCCGCTTGGTATGTCGAAGGTAGCGCAAACAGGATCGGTAACCGGCCGTGTTACGAATACGATCACGAATTGTTAAAAATTTACACCAACAAAACTATAAAAGAAATAATCGACGCTACCTATTCGTCGCCCTTCCTTTATGGAATGGGCAGTGTGCTGGTCCAATATTTGTACGAAAATCGACCTGCCGATTTCAGACACATGATAGAATCAAAGAATTACTCCGTTAGCGAAACTTACGAAACGGATTTTGATGTGTTCAAATTAAATTTAATCGGTAGATGTGAAGCTGTGAAGAGAAATCAAACTGAACACAAGTTTGATAGCCAGCTGGCGTACAAAAATTTAATAGACTCGAGCACGTTTGCCTCTTGTAAAAATTACATTCGATTCGATTTTGACGATATTATTTTCGTGTTAACACCGGACCGATTGATAAAACGAAACAAAAACGGGCAGCCCATGCTCGAATATACTCAATATTTGATCGGTCAAACTCATTTTGATGAAATCAACGAGTACACCTTTGCTTGGTTCATGGCGGGATTGGTTAAAAAGGCGGTTCAGTACTTATCAGACAATGTAAATTTTTATTACAAATATCAGTCTAGCTATACGTATGATTCGACGGTCAAATGTCAGTATATCGACGAGTCTGCCAAAGATGCCATTATAAATATGGCGTTTAAATATAAAAACATCGCCGATATGGTCACGTTTCCCGTCGATCAAGCAAAGGAATACATACGCCAAAAAGACAAGGCGATTGAAATGTGTGAAATGTACATGCCGCCCGTACTGCTTAATACGGCCGGGCCTGCCAAAGTATTCATCGATAGTTTGGCAAGTCCCAATTTAGTGATCCCGAGAAAAGAAACATATTATATGCGCATCGATTTAAAAGGGAACACAGTAATTCATTATGCTGCCATGTACAAAATAAATGCCTACGAAAAAATCAAACTGGCAAACAGGACAGCCGTAGATATGGCGCCTCTCAATGCCGACGGAAAATCGGCCGAGCAATTGTACGAATATAGTTTAAAGTTTTACAAAAAGTACAACCAAAGTAATCCTTTATATTGCTTTAAATATATGGAAGATACAGAAAGTATCACAGAAATTATTGAAGAGGAGGTGGTGGAAAAGGAATATGAAGGAAACAATACAGTTTTATCAAACAACCCTTTATTAAGTAAAAGCACATCAGTTATAACAGAGAAAAGCATTATTATAACAGAGATTACTACAACAGAGAAAAGCCTTATTATAACAGAGAAAAACATTATTTCAACAGACAATAACTTTAGCATTACATTTAGTTTCAAAAATTGTATAATTTTCATACTTGTATTATTAATATCATTTATAGTTATAATACTGTTAAACGCATTGGTAACTTTAATAATAACAAAAAAAATCACTAAGCAAAAACAAAAAGTGCAACAAAAAGTTGAATATAACAAAGATAAATTTTATACTAACGACGAATGTACAATTAATTTATTTGATTAAATACTGTTAAGGAACCATCGTTTTTTATTTATATTCAAAAACAATATCTATAGTTAGTAGTAAAGGACCCGTTTTTGAATTCACAGGGGCCCATTGAGTCGTATTGGCAAGATTACGGACGTTTAGTTCGCACTCGTTTCTTTCCACATCCTCTTCGTTGATTTTGGACAACAAATAGTTGTCGTAAACTTGTCCATCGACACTGTCCTCTTGTTTGCGATGATTGAACCATAATTTATTGTACCACTTCACATCAGATACCATAATAGCCTCATTTATGTATACTGTTAAAATTGTCTTGTCTCCCATTGCTCGCGCCATTCCCTTGACCGAAATCAAATTGTGATTTTCCTTTAGCGATTTTATCTTGCTCAATATAAATGGTGTGGTTTTAGCTTCATCTTCGCGACTCGAAAACCCATCAAAGTAGATACATTCTTTCGGCGATTGAGCGTTCACTATACTTATGTGTTCTTTTAAATATTTAAAAGTGGCCTGGATGTAGCTGTTACTAGAGTCAATTTCCACTTTTACCGTTTTGGATTTATTGTCCTCTATTAAATATTTATATGTTATTTTGTTAAGAAGTCTGTCCGCGCTAACCGATGCATGCTCATTTTTTATGTCGTCCAAATACAGAGTAACAGGATATTTGCATAGTTTAAACATTTTTAAATATGCGTATTATTTCCTGTGCAAAAGCCTCCGCCTCCTGGTAATGATGAAATTTTATTCTTGTCGGGGTAATATCTACGCGCGTACCAAACTTAGCTTCTGCCAAATTTGCACAGATTACGCGTGCTTCGCTCGGATCATCACTTACTCTGTACGCTAAAATCCTGCATTCCTTAGTGATCTCACTGATTTTTGTGTAAAATCTAATATAATTTAACTCTTTCACAATATAAACATAATTTTTTACCTTTGACTGAAGTTGTTTTATTTTTGTGTCTTTTCTTCGTATAGTATTCTTTAATTTTTCTGTCGATATTTCAAACTCGTGCTTGTCGCGTTTGTTTTTTTCACACATTTTTTGGTATGCTTGTTTTAAAAGCTCAAGTGCACGAGCAATTTTTTTCAAGTCTTTAACAGTAACAAAACGAAACATTTCACCACTTTCAATATTAGCAGCGGTCAAACTACGGACTAGACTTTTGTTTCGTAGAGTAGACATGTTTCGCGACGGTCGAACACCGAATGAAATTTGAAAGGCGAACGCGAACGTCGTCATATTTATCATCTGACGACCACGCAAAGACACGCGATAGCTATTCCTGGAAACGAGGAAAAAAAAATACAAACATGGTAAAAACAACATTTATTATGCGTAAATCATTTTTAACAAATTTTTAAACTGATTCATAATTGTGTAGGTGACAAACTCAATAATCATATTAGTCATGTAATACTCATTGTAATCAACATTGGCCGCCTTTGTTTTGAAAATATTCTTGCTGTTTTTTAAATTGATTACGTAATCGGTGTACCAAACGGCCTTCCGGATAGGTGTGATCGGTTGATGACGAATATTATGTCGCAATTCTAGTATGTTTTTGCGATACAACGGATCATTGATGACAGTTTTGATCGCTTCCGTTAACTGGTTGGAGTCCACGGTGGCGGTGTCGAGTGCCAGCCCAATGCCCAATTCTACATACTTGTCGGTGTTAAACGACTGATCGCCCATCATCGGCAAACCTACCATCGGTACACCGGCGTCGATAGCTTCGTCCGTGGATTGAACCCCTCCTTGTGTCACAAACGCTACAACATTTTTATGTTTTAGCACTTCCGGTTGGTTGAACCAAGTTTGCACCAAAACGTTGTAAGGTAATTGAGAGTTAAGTTCTTCGTCGTCCGTTTCAAACTTGAATAAAATTTTGTAGGGTAGCGCTTTAAAAGTTTGTAATAGCATATATTTAAAATCAGCATCGGTGTCTTTGGTGTTTATGCTCGAGCCGAAACTAACGTAAACCGCTCCTTGAGTGGCGCCGTCCAAGTAACGCTTCATGAAATCGTCCAGCGGCACGGGCTCGTTTCTATGCAAATGAATTCCACCCAAGTATTGAACACTAGGCGGAACCGGCCTGTTATTGTCAAAAACAGAGTGTGTATTAACAAATAACATCTGGACACGATTGCGCAGTTCCTTGATAGACGGCGATTTTCGACCGAATTGCGAGCGTATTAATTTATTCTGCTCGTCCACCAATTTTGAAAACTCATTTTGTAGCGTCAACTCCATGTAAATTTCGTTGATAGTTTCCCATACCGTTAAATCCCTGAACCGATCGCGCCACATGTTCGGATAGTATTTCGGGTGGCGGCTAACTGCACCCATAGTTTCAAAATTTTCTGCCAGTCCGTAGCCCGATGAAATTTGAATTACGGGCAGGTCACCGAACAGATGAGAAAATATTAGCGAGTAGTCCATAAAAGCCTCGCAAATTAGCAAATCGAAATGCTCATTCTTTCGATTATTGATTAGCCTCTTGACGGCCGGCAAGTCAAATTGATCTCTAATCATGCGCACTAAACCCATGTAGTTGTGCGCCGTGACGGTAGTACTGTCCGATATTATTCCCCTTTTTCGGAATACGCTAGATTGTTTAACCAGGTTTTTAAAGTAATTTTCGGCAAGGGTGGCATCGACTTCTGTCACATTTCGGGTAGCGTAATTGACTTTGGTAGTGGGTTTTATCACGACAATTTCGTGGCCTCTTTCCGCCAAAGCTTGTATGTAAACTTTGAACACACTCTGGTGGCTGTAGGCGGGTGTTGGTAAAACTGCGAGAATTCTCGCTCCATTTACTGCAGAAATGGCACACAAAAATAGAATATGGAAGTGCATGACGAACAATATTCGGAATAATTTGTGTCAGTAGGCCAACGGTTTATATACTTTTATTGTGTTTCAACGAAAGTGATTCTGGAATGTTGGTCATTTTATTAAAATTATAATTTAGCTCGTTAATCTGTATAGTTTCCAGTTGCTCCTTTTCGTTGCTCTTTTTGCGTTTACAACACATTGAATATTTGTAAAATTTAATTTTTATCAAAAGAACCGAAATTAGCACTAATAATATCAAAACGATTTTTGTATAATTAACATCGTTTACTTCGTTTTTCTGAATGTCGGTGAGTTCATCGACAATAATATCGAGAATTTTATCTAGCTCGTCTTGCTCACTGCCAACGGTTACGTTGATGAAATCGTCCGAACTGTTTTCGCTGTACGTAGTACTGATAAAAATAGATATGATAAAGAAAATCTGAGTAATCGTCATATTTAATTTTTTTCTTAGTCACTACTTGATATTTCCAGAGTCAACATCATGTCTCGGCGTTTCGCGATATACACCAGCGAAAAGGCGAAGATGGTTTGGGACTCGGTAGCATTCAATAATAGTCGCCAATTTGCCTTTTTCGATGGCACACACTGGTATCATCCCCAAAATCATTTTGCAGACTTTGATGAGTTTTTCAACTACTTGAATGAAAACAATATCAAAGATGTGCATGTAAAAGCGCTCGAGGACAACGGAGGACGCGAGTGGGTTATCGATGTGGATTTCATCGAAACTGGAAAAATATTGGAGTTTAAAATTGAAACTGCCAAAAAGATTTTCATCAATTTTTACAAGGAAAACATTGCACGGATAATGCACTCCGGGAATCGAGGACTACATGTCTGGTTGCGCATAGATAGATTTTTAATGTCGTCAAAGAAAAGTTTGCGCACCAGCTACTACTCGATATTTGTGCAACCCGAAACTGTGATTTTAGACGAAATCGTGCCCGGTAGTTTCATTCATGCTGTGAAAACCGCAGTTGAAGAAGAAACCGTTGCTCAAAAGATCGAAGAATATTTTAAGGTGAAAACTTTGGAGCAAAGAATATTAGCCGTTTGGCCCCAAGTGGACTCACACGTGTTTTGTAACTTAAACCAAATTCGTGCTCCGTTTAGTTTTAATTCTAAGGGTCGTGATTTCTCCAAACAATTGTACTAGTAATGTTAATAAAGATGTTAAGCAAAATATTTTCGTTTGTTTTCAATAATAAAAATGACGAGGAGAACATCGAACATGAAGAGGAAGACACCGATCGGCCGCGCCGTCTGCCCGCCGTAGGTTTTTCGTATCTGTTTCAGAAAAAAAATTTCATTTTCGATGAAAACATATCGTTTTTATTACGATACTTGTATGACAAAGACGAAGTTTGGATTTTGTATTTCGATCTGTCCGACGGGGTGGGTCTGCCAGAAGACGCCCGTGACTCTATTAGTTATCAGAATATCAAAACTGTCAATGAACTTTTGCCCAAACAGTCTTCGCAAATTAGCAGAGTGGAATGTATTAACAAGCATGGCATGTTGGAGGCGCTAGAATCGATTAGCTTTAAATCCAAGGCACAATTAGTGACAAAGCTGTTGGATATGTTTGCGGAATTGGAATCGAAATCGACGCCGCTATCTAAAATGGACCATAAATTTGACGTCCTATTAAAGGCTATACAAGAAGTCAAAAATGACAATGCCAATTTGTTTAAAATACAATTGGATCGATGTCAAGATTTCGAGCGCAAAATAACAGAACTGGACAAAAAGATTAGCTTGTATGAAAATATCGATCATCTTTACGAGCGGCTGCGTGAACGGCACAAAGCGACGGCATCGGACAGACACATGTTTCTAGATGACAATAACGAAATGACCATTAAGCTACCAAAAGATGTATCGAAACATCCTCGAATAGCAGTGTTTATGAAACCCTCACAAGCTGGAACAAAAGTTGCATTCGTCTCTGGCCAAAAGAAGTATTTGCAAAAAGGGAAACGAAAATTTCAAGATATGGAGTTGGTGTACGACGGGGTTCATCCGAATCCCCAGATGGCTGTAAATTGTATAGCCGAGGAACTAGACTTTAAAAATTTTGATTATATTAAAAAGGCTCGTTTGTATTATTTAAACTGTAACTTGGAAACGGCAAAGTCGTTTATAAAAGAAAATTTGTAATAAATAAATTTGAATATACACTTTTGTTATCAGTTACGCCTCCGTTTTTTATCTATTCCTCCCTATAAAAGAACAGATTTTCAAAATAAAATCACAGTACAGCAACATGTTTGTGGTTAAACGTAACGGTCGTAAACAACCCGTCCTCATGGACAAAATCACTAAAAGGCTTGAAAAGTTATGTTTTGGACTAAACATCAAAGCCGTTTTAGTGGCGATGGAAGTGGTCAATTTTATTTACGAAAATGTAACCACCGAAGAATTGGACATGCAAGCGGCAAAGGTTGCTGCAAACAAATCATATTTGCACAATGATTACGCCTTATTGGCAGGCAGAATCATGATTAGCAACCTGTCGAAAAAAATCAAGTCTGATTTTTCTGAAGTTATCGAAAGATTGTACAAATACAAATTGGTTACTAAATTTTTGTACGATGTAGTATGTGCCAACAAAAAAGTTCTCAACGAATGTATACTTTACGAGAACGATTACGATTACAAGTACTTTGGTTTTAAAACCTTAGAAAACGGGTACTTGTTTAAAATCGATGAGCAGACCGCTGAATTACCGCAGCAAATGCTGATGAGAGTTTCGCTCGGAATTCACGGCGACAACATTGAAGATGCCATTGAAACATACAAACTGCTGTCTGAAAAAAAATTCATTCACGCCAGTCCCACACTATTTGCGGCCGGAACAAACTTGCCCCAATTATCATCGTGTTTTTTGGTGAGCATGAAAGAAGACAGCATAGCCGGCATATACAACACGCTAACCACTTGCGCGTTAATTTCGAAACATGGCGGCGGAATAGGACTGAACGTACACGAAATTCGCGCTCGTAACAGTCCCATCAAAGCGACCAACGGAACTTCGAGCGGACTAGAGTCTATGCTTCGCGTATTCAATAATATGGTACGACACGTTGATCAGGGCGGCAAAAGAAAAGGCGCCATGGCAATTTACTTGGAACCGTGGCACGCCGACATTTATGATTTTTTAAATCTAAAAAGAAACATGGGCGCCGAAGACAAAAAGGCCCGTGATCTTTTGTACGCTCTATGGGTACCTGATTTATTTATGAAACGTGTAGAAAATGGCGAAATGTGGTCACTCATGTGTCCCGACGCTTGCAAGGGATTGGATCGAATTTATGGAAAAGAATTTGAAGAGTTATATTCGCGTTATGAATCAGAAAAGAAATACGTGAAGCAAGTGAACGCGCGCGATCTTTTCAGATTCATAATTGAAACCCAAGTCGAAACCGGTACACCGTACATGCTGTACAAAGATGCTTGTAACGGTAAAAGTAATCAAAAAAACTTGGGCACAATAAAATGTAGTAATCTTTGTGCCGAAATAGTACAATTCAACAGTGGTGACGAAACAGGCGTTTGCAATTTGGCATCTATTTCGGTCAACAAGTTTGTTTGCGATAATTTCGAGTACGATTTCAAGTCGTTAAAAAAGGTGACCAAAGTGATTGTGAAAAACCTCAACAAAATAATCGATGTCAATTTTTATCCAGTAAATTGTGCCCGCGATTCGAATTTCAAACATCGACCTATCGGCGTGGGTATTCAGGGGCTGGCCGATACTTTTGTAATGCTCAGACTGCCGTATGAAAGTGACAAGGCCAAGTTGCTAAACAAACAAATAGCAGAAACCATTTATTATGCCGCGCTGGAAGCTAGCTGCGAGCTCGCAAAAACTGACGGGCCATATTCGTCATACAAAGGCAGTCCGGCCAGCGAAGGTTTGTTACAATACGATTTGTGGAATGTGACTCCTACAAAACTTTGGGACTGGAAAGTTTTGAAAGAGAACATTAAATTGTACGGACTCCGCAATTCTTTGCTGGTAGCGTACATGCCTACCGCTACGACGGCTCAAATACTCGGCAACAACGAATCTTTCGAACCTTTCACTAACAACTTGTATGTGCGCCGAGTGCTGGCGGGCGATTTTCAAGTTGTAAACCAATATTTGGTGGATGATTTGTTGCGTCTCAATTTGTATAATGATAATATGCTCAACAAAATTATCGCCAACAACGGCAGTATTCAAGCCATCGAAGGTATACCTGCCGATATCAAAGAGTTGTATAAAACCGTGTGGGAAATGAAGCAAAAAAATTTGATTGACATGGCCGCCGATCGCGCAGCGTTCATAGACCAGAGTCAATCATTTAATGTTTTCGTTGCCAATCCCACTTATTCTCTGATGACATCGATTCATACTTACGCTTGGAAAAAAGGATTGAAAACGGGAATGTATTATTTGCGCACCAAGCCTGCCGCGGATCCTATCAAGTTTACTGTGGACGCCACATGTTCTAAAGTGAACGGTCAATGTATGGGATGTGATGCTTAATAAAATTTGTAATAAAAATTTTCATTGTTTTATTTTTTTTATTGTAAAAATGAAGAAATATTTTTCGTGTCTTTCTTCATTTTTACAAGCAAATAAGTATTTTTCTCCTTTCGTAAAACATTGTGAAAAATCAAATATA

The invention claimed is:
 1. A biopesticidal composition for use incontrolling insect populations, the composition comprising: ahomogenized mixture of: larvae infected with a nucleopolyhedrovirus(NPV) having a genome sequence with at least 98% sequence identity toSEQ ID NO: 2; an artificial larval diet on which the larvae were reared;and occlusion bodies of the NPV from the infected larvae; water; and anadditive which improves the stability or maintains the activity of thevirus in the composition; wherein prior to being homogenized, the larvaehave been cultivated, fed on the artificial diet, inoculated with aninoculum of the NPV, maintained on the artificial diet to allow thevirus to replicate within the larvae, and harvested.
 2. A biopesticidalcomposition as claimed in claim 1, in which the NPV has a polyhedringene with more than 95% sequence identity to SEQ ID NO:
 1. 3. Abiopesticidal composition as claimed in claim 1, in which the NPV is anisolate or genotype of an NPV whose genome sequence is represented bySEQ ID NO:
 2. 4. A biopesticidal composition as claimed in claim 1, inwhich the NPV has a virulence host range comprising the Grapholitinitribe of the lepidopteran family, Tortricidae.
 5. A biopesticidalcomposition as claimed in claim 1, further comprising one or moreadditional biopesticide.
 6. A biopesticidal composition as claimed inclaim 5, wherein the additional biopesticide is selected from the groupconsisting of a Cryptophlebia leucotreta granulovirus, a Lydia pomonellagranulovirus, a Cryptophlebia batracopa granulovirus, and a Grapholitamolesta granulovirus.
 7. A biopesticidal composition as claimed in claim1, further comprising a feeding stimulant, a mineral oil, and/or a UVprotectant.
 8. A method of controlling insect populations, the methodcomprising applying to the insects or their locus an insecticidallyeffective amount of a biopesticidal composition comprising: ahomogenized mixture of: larvae infected with a nucleopolyhedrovirus(NPV) having a genome sequence with at least 98% sequence identity toSEQ ID NO: 2; an artificial larval diet on which the larvae were reared;and occlusion bodies of the NPV from the infected larvae; water; and anadditive which improves the stability or maintains the activity of thevirus in the composition; wherein prior to being homogenized, the larvaehave been cultivated, fed on the artificial diet, inoculated with aninoculum of the NPV, maintained on the artificial diet to allow thevirus to replicate within the larvae, and harvested.
 9. A method asclaimed in claim 8, which comprises simultaneously or sequentiallyapplying, in addition to the biopesticidal composition, at least oneadditional biopesticide to the insects or their locus.
 10. A method oftreating or preventing insect infestations on plants or fruit, themethod comprising applying to the plants or fruit an insecticidallyeffective amount of a biopesticidal composition comprising: ahomogenized mixture of: larvae infected with a nucleopolyhedrovirus(NPV) having a genome sequence with at least 98% sequence identity toSEQ ID NO: 2; an artificial larval diet on which the larvae were reared;and occlusion bodies of the NPV from the infected larvae; water; and anadditive which improves the stability or maintains the activity of thevirus in the composition; wherein prior to being homogenized, the larvaehave been cultivated, fed on the artificial diet, inoculated with aninoculum of the NPV, maintained on the artificial diet to allow thevirus to replicate within the larvae, and harvested.
 11. A biopesticidalcomposition as claimed in claim 1, wherein the additive which improvesthe stability or maintains the activity of the virus in the compositionis glycerol.
 12. A biopesticidal composition as claimed in claim 1,wherein the NPV has a genome sequence with at least 99% sequenceidentity to SEQ ID NO:
 1. 13. A biopesticidal composition as claimed inclaim 1, which has a concentration of NPV of at least 1×10³ viralocclusion bodies/mL.
 14. A method according to claim 8, wherein theadditive which improves the stability or maintains the activity of thevirus in the composition is glycerol.
 15. A method as claimed in claim8, wherein the composition has a concentration of NPV of at least 1×10³viral occlusion bodies/mL.
 16. A method according to claim 10, whereinthe additive which improves the stability or maintains the activity ofthe virus in the composition is glycerol.
 17. A method according toclaim 10, wherein the NPV has a genome sequence with at least 99%sequence identity to SEQ ID NO:
 1. 18. A method as claimed in claim 10,wherein the composition has a concentration of NPV of at least 1×10³viral occlusion bodies/mL.
 19. A method as claimed in claim 10, whereinthe insects are selected from the tribe Grapholitini.
 20. A method asclaimed in claim 10, wherein the insects are selected from the larvae ofthe group consisting of Cryptophlebia peltastica, Thaumatotibialeucotreta, Lydia pomonella, Grapholita molesta, and Thaumatotibiabatracopa.